ABSTRACT
CD39 (ecto-nucleoside triphosphate diphosphohydrolase-1; E-NTPDase1) is a plasma membrane ecto-enzyme that regulates purinergic receptor signaling by controlling the levels of extracellular nucleotides. In blood vessels this enzyme exhibits a thromboregulatory role through the control of platelet aggregation. CD39 is localized in caveolae, which are plasma membrane invaginations with distinct lipid composition, similar to dynamic lipid microdomains, called rafts. Cholesterol is enriched together with sphingolipids in both rafts and caveolae, as well as in other specialized domains of the membrane, and plays a key role in their function. Here, we examine the potential role of cholesterol-enriched domains in CD39 function. Using polarized Madin-Darby canine kidney (MDCK) cells and caveolin-1 gene-disrupted mice, we show that caveolae are not essential either for the enzymatic activity of CD39 or for its targeting to plasma membrane. On the other hand, flotation experiments using detergent-free or detergent-based approaches indicate that CD39 associates, at least in part, with distinct lipid assemblies. In the apical membrane of MDCK cells, which lacks caveolae, CD39 is localized in microvilli, which are also cholesterol and raft-dependent membrane domains. Interfering with cholesterol levels using drugs that either deplete or sequester membrane cholesterol results in a strong inhibition of the enzymatic and anti-platelet activity of CD39. The effects of cholesterol depletion are completely reversed by replenishment of membranes with pure cholesterol, but not by cholestenone. These data suggest a functional link between the localization of CD39 in cholesterol-rich domains of the membrane and its role in thromboregulation.
Subject(s)
Antigens, CD/chemistry , Apyrase/chemistry , Cholesterol/chemistry , Lipids/chemistry , Actins/chemistry , Animals , Antigens, CD/biosynthesis , Antigens, CD/metabolism , Apyrase/biosynthesis , Apyrase/metabolism , Caveolin 1 , Caveolins/genetics , Cell Line , Cell Membrane/metabolism , Cholesterol/metabolism , Cricetinae , Detergents/pharmacology , Dogs , Dose-Response Relationship, Drug , Endothelium, Vascular/metabolism , Filipin/pharmacology , Fluorescent Antibody Technique, Indirect , Humans , Immunohistochemistry , Lung/metabolism , Membrane Microdomains/metabolism , Mice , Mice, Knockout , Mice, Transgenic , Microscopy, Confocal , Nystatin/pharmacology , Placenta/metabolism , Plasmids/metabolism , Platelet Aggregation , Protein Binding , Protein Structure, Tertiary , Spleen/metabolism , Sucrose/pharmacology , Time Factors , Transfection , Umbilical Veins/cytologySubject(s)
Kidney Diseases/urine , Kidney Tubules/physiopathology , Rhabdomyolysis/complications , Trypsin Inhibitor, Kunitz Soybean , Biomarkers/urine , Drug Therapy, Combination , Gemfibrozil/therapeutic use , Humans , Hyperlipidemias/complications , Hyperlipidemias/drug therapy , Kidney Diseases/etiology , Kidney Diseases/physiopathology , Magnetic Resonance Spectroscopy , Male , Membrane Glycoproteins/urine , Pyridines/therapeutic use , beta 2-Microglobulin/urineABSTRACT
Prothymosin alpha (ProTalpha) is a histone H1-binding protein localized in sites of active transcription in the nucleus. We report here that ProTalpha physically interacts with the CREB-binding protein (CBP), which is a versatile transcription co-activator. Confocal laser scanning microscopy reveals that ProTalpha partially colocalizes with CBP in discrete subnuclear domains. Using transient transfections, we show that ProTalpha synergizes with CBP and stimulates AP1- and NF-kappaB-dependent transcription. Furthermore, overexpression of ProTalpha enhances the transactivation potential of CBP. These findings reveal a new function for ProTalpha in transcription activation, probably through CBP-mediated recruitment to different promoters.
