Der f 34, a Novel Major House Dust Mite Allergen Belonging to a Highly Conserved Rid/YjgF/YER057c/UK114 Family of Imine Deaminases.

The high prevalence of house dust mite (HDM) allergy is a growing health problem worldwide, and the characterization of clinically important HDM allergens is a prerequisite for the development of diagnostic and therapeutic strategies. Here, we report a novel HDM allergen that belongs structurally to the highly conserved Rid/YjgF/YER057c/UK114 family (Rid family) with imine deaminase activity. Isolated HDM cDNA, named der f 34, encodes 128 amino acids homologous to Rid-like proteins. This new protein belongs to the Rid family and has seven conserved residues involved in enamine/imine deaminase activity. Indeed, we demonstrated that purified Der f 34 had imine deaminase activity that preferentially acted on leucine and methionine. Native Der f 34 showed a high IgE binding frequency as revealed by two-dimensional immunoblotting (62.5%) or ELISA (68%), which was comparable with those of a major HDM allergen Der f 2 (77.5 and 79%, respectively). We also found that Der f 34 showed cross-reactivity with another prominent indoor allergen source, Aspergillus fumigatus This is the first report showing that the Rid family imine deaminase represents an additional important pan-allergen that is conserved across organisms.

Remineralization of primary tooth enamel from individuals with Down syndrome.

PURPOSE: The purpose of this study was to clarify the characteristics of primary tooth enamel of Down syndrome patients (DSPs). We examined 9 primary teeth of Down syndrome children and 11 primary teeth of normally developed children to investigate the remineralization processes of enamel by transverse microradiography and X ray micro analyzer (XMA). METHODS: Mineral loss, lesion depth, maximum mineral value, minimum mineral value, depth of maximum mineral value, and depth of minimum mineral value were used to analyze transverse microradiography (TMR). In addition, we calculated the percentage of enamel remineralization. RESULTS: All the parameters in the 2 groups showed marked recovery. The results indicated that the Down syndrome group was significantly remineralized the same way as the control group. According to the comparison of mineral content distribution by XMA, the content distribution of magnesium was different between the 2 groups. CONCLUSION: While recovery through remineralization of primary teeth was similar between Down syndrome children and normally developed children, the mechanism of remineralization process may be different between the 2 groups; consequently, magnesium may be considered as one of the factors affecting recovery.

Relationships between mandibular cortical bone measures and biochemical markers of bone turnover in elderly Japanese men and women.

OBJECTIVE: The aim was to clarify the association between dental panoramic radiography measures of mandibular inferior cortical shape and biochemical markers of bone turnover in elderly men and women. STUDY DESIGN: Subjects were 80-year-old men (n = 85) and women (n = 153). Mandibular cortical shape and width were evaluated on dental panoramic radiographs. Bone formation and resorption, respectively, were estimated by serum levels of carboxy-terminal propeptide of type I collagen (PICP) and serum type I collagen carboxy-terminal telopeptide (ICTP). RESULTS: Eroded cortices of the mandible were significantly associated with increased serum PICP levels (P = .005) in women. Lower mandibular cortical width quartiles were also significantly associated with increased serum PICP levels in men (P = .020) and women (P = .006). CONCLUSIONS: Mandibular inferior cortical measures detected on dental panoramic radiographs may be associated with bone formation rates and be useful in predicting osteoporosis in elderly Japanese men and women.

Temporal association of elevated C-reactive protein and periodontal disease in men.

BACKGROUND: Most studies on the relationship between serum C-reactive protein (CRP) and periodontal disease have been cross-sectional. In this study, we investigated the temporal association between CRP and periodontal disease by following a large number of subjects for 1 year. METHODS: We studied 11,162 men in Nagoya, Japan, who had an initial dental examination as part of a complete physical examination and then underwent the same examination 1 year later. For the 4,997 men without periodontal disease at baseline, logistic regression analysis was performed to examine the relationship between baseline CRP and periodontal disease 1 year later, adjusting for age, body mass index, glycosylated hemoglobin A1c level, and smoking status. Similarly, logistic regression analysis was performed to examine the relationship between periodontal disease at baseline and CRP 1 year later for the 10,376 men with normal baseline CRP, adjusting for the same confounding factors. RESULTS: Among men without high CRP at baseline, periodontal disease at baseline correlated to CRP 1 year later. The odds ratio was 1.336 (95% confidence interval [CI]: 1.115 to 1.674). However, in the men without periodontal disease, no significant correlations were seen with baseline CRP or periodontal disease 1 year later. The odds ratio was 1.163 (95% CI: 0.894 to 1.513). CONCLUSION: Periodontal disease increased the risk for high serum CRP levels in men after 1 year of follow-up.

[An intervention comparison of dental hygienist visits and leaflet mailing for improvement of oral health scores in village residents].

PURPOSE: This study was undertaken to clarify to what extentintervention by dental hygienist visits and leaflet mailing, once or three times a year, increased the dental health scores of Tobishima Village residents in a 3 year period. METHODS: The oral health status and oral health score (the Oral Salutogenic Score) of 786 residents were examined in May, 2004. Of the total, 446 people had oral health scores equal to or less than 15. They were divided into three groups at random, receiving: dental hygienist visits (visit group); leaflet mailing three times a year (three times mail group); and leaflet mailing only once a year (single mail group). Oral health status and scores were examined a second time by dentists in May, 2007 and average increases of scores over 3 years were calculated for each of the three groups. The Mann-Whitney test and cost-effectiveness analysis were employed for comparisons. RESULTS: The average increase in oral health score was 46.2 +/- 8.1% for the visit group, 31.2 +/- 4.4% for the three times mail group and 25.0+/-5.2% single mail group over 3 years. The visit group value was significantly higher than the single mail group (P<0.05). Of the resi-dents who had an oral health score equal to or less than 15 points, increase of their oral health score those who received a dental hygienist visit had a higher than the groups who were sent leaflets. With the latter, the greater frequency of leaflet distribution was more effective for increasing the oral health score. CONCLUSION: It was concluded that visits by dental hygienists are more effective than mailing of leaflets. In addition, mailing 2 or 3 times a year is more effective than once a year.

Effect of strontium in combination with fluoride on enamel remineralization in vitro.

Previous studies showed that strontium (Sr) as well as fluoride (F) can enhance enamel remineralization. The aim of this study was to evaluate the effects of Sr in combination with F on enamel remineralization in vitro. Sixty enamel specimens obtained from caries free human premolars were demineralised to produce caries-like lesions. Half of each lesion was covered with nail varnish as an untreated control. The specimens were then randomly divided into F and Sr+F treatment groups. The F group was exposed to remineralizing solutions (1.5mM CaCl(2), 0.9 mM KH(2)PO(4)) containing 1 ppm, 0.1 ppm or 0.05 ppm F. The Sr+F treatment group was exposed to the same solutions including 10 ppm Sr. After 2 weeks, lesion depth, mineral loss and percentage enamel remineralization were determined using transversal microradiography. There was a significant decrease in mineral loss in all groups (p<0.001). Lesion depth was significantly reduced for all groups (p<0.05) with the exception of group F. Remineralization was significantly affected by F concentration (p=0.000). The participation of Sr resulted in a significant enhancement of remineralization (p<0.001) with a synergistic effect of the Sr+F combination (p<0.01). It was concluded that while the remineralizing process was affected by the concentration of F, there was also an interaction between F and Sr when they were used in conjunction.

Gradients in periodontal status in Japanese employed males.

AIM: The objective was to assess whether there was a social gradient in periodontal status by job classification in Japanese male workers. MATERIAL AND METHODS: Study participants were 15,803 Japanese male workers aged 20-69 years. There were seven groups classified by jobs. Oral examinations were conducted using community periodontal index (CPI). RESULTS: The percentages of sextants with a CPI score of 3 or 4 of professionals were significantly lower than for other job classes. The gradient also existed for the percentage of sextants with CPI score 4. After adjusting for age, a history of diabetes mellitus and smoking status, those lower on the gradient, namely, drivers, service occupations, salespersons and managers were 2.0, 1.5, 1.4 and 1.4, times, respectively more likely to have one or more sextants with CPI score 3 or 4 compared with professionals. Odds ratios of having more than one or more sextants with CPI score 4 after adjusting for age, clinical history of diabetes mellitus and smoking status for drivers, service occupations, salespersons and managers were 2.1, 1.5, 1.5 and 1.2, respectively, compared with professionals. CONCLUSIONS: There was a gradient in periodontal status by job classification. Professional and office workers had better periodontal status than salespersons, service occupations and drivers.

Is there a gradient by job classification in dental status in Japanese men?

The objective of this study was to assess whether there is a gradient in dental health status by job classification in male Japanese workers. The study subjects were 16,261 male Japanese workers aged 20-69 yr. Jobs were classified into seven job groups. Oral examination was conducted using World Health Organization criteria for decayed, missing or filled teeth (DMFT). Professionals, managers, and office workers had a better oral status than those in service occupations and drivers. Professionals had significantly more natural and sound teeth than those in other job classes. The DMFT of professionals was significantly lower than in workers of other job classes. Professionals had a significantly higher restorative index than did workers of other job classes. The restorative index of office workers was significantly higher than that in managers, skilled workers, salespersons, those in service occupations, and drivers. Drivers were 1.8 times, and those in service occupations 1.3 times, more likely to have one or more carious teeth compared with professionals. Skilled workers, salespersons, those in service occupations, and drivers were 1.3, 1.3, 1.4, and 2.1 times, respectively, more likely to have a missing tooth compared with professionals. Skilled workers, those in service occupations, and drivers were 1.7, 1.9, and 3.1 times, respectively, more likely not to have 20 or more teeth compared with professionals aged 50-69 yr.

Salutogenic factors that may enhance lifelong oral health in an elderly Japanese population.

OBJECTIVES: The objective of this study was to assess which factors affected the maintenance of more than 20 teeth in Japanese patients aged 80 years and older, using a life-course perspective. SUBJECTS AND METHODS: The oral examination was carried out by dentists. The questionnaire asked about family background, background during various stages of growing up, tooth brushing, snacking, job history, health examination, war experience, number of children, oral health condition and dietary preferences. Some of the questions asked about conditions when they were a primary school student, 20 years old, 40 years old and 60 years old. Multivariable logistic-regression analysis was used to adjust for sex at significant life stages. RESULTS: The group with mothers who did not prefer sweet food had an approximately four times greater chance of having >or=20 teeth compared with the group with mothers who preferred sweet food. Those participants who did not prefer sweet food themselves were approximately three to five times more likely to be in the >or=20 tooth group compared with those who preferred sweet food at all stages of their life course. Non-smokers and those who quit smoking were three times more likely to be in the >or=20 tooth group than those who smoked throughout the years from 20 to 60 years of age. CONCLUSIONS: Factors associated with the maintenance of a sufficient number of functioning teeth were: not having a mother with a preference for sweet food, not having a preference for sweet food themselves and not smoking over a long period.

Relationship between survival rates and numbers of natural teeth in an elderly Japanese population.

OBJECTIVES: The objective of this study was to assess whether elderly people with 20 or more natural teeth were more likely to live longer than a cohort with less than 20 teeth. MATERIALS AND METHODS: Groups of elderly people over 80 years of age (24 males and 35 females) with 20 or more teeth (>or=20 group) were compared with elderly people (24 males and 35 females) with less than 20 teeth (<20 group). Follow-up studies were conducted at regular intervals for 10 years from July 1992 to July 2002. The cumulative survival rate of the >or=20 group (average +/- SE tooth number of teeth - males, 23.9 +/- 0.6; females, 23.8 +/- 0.4) was compared with the <20 group (average number of teeth - males, 3.8 +/- 1.1; females, 2.6 +/- 0.8). The multivariate Cox proportional hazard models with the number of teeth in a group (>or=20 group or <20 group). Smoking status and alcohol intake as covariates were used to adjust the cumulative survival rate. RESULTS: The male participants in the >or=20 group had a significantly higher cumulative survival rates (p < 0.05) than the <20 group at 18 and 21 months from baseline. There were no significant differences in survival rates between the female groups. Adjusted cumulative survival rate was significantly different at 72, 75 and 78 months between the >or=20 group and <20 group for males but not for females. CONCLUSION: Having 20 or more natural teeth was associated with increased survival rate in elderly males, but not among the elderly females.

The effect of fluoridation and its discontinuation on fluoride profiles in the alveolar bone of rat.

We investigated the effect of fluoridation and its discontinuation on fluoride content in the alveolar portion of the mandible in rats. Drinking water with three different fluoride contents (0, 50, 100 ppmF) was given to rats for three different periods (4, 13 and 25 weeks). Fluoride concentrations were measured in the crest, the middle, and the apical parts of the alveolar bone and in the body of the mandible. Furthermore, after fluoridated drinking water was given to rats for 4 or 13 weeks, distilled water was given to them for 21 or 12 weeks respectively; and the effect of the discontinuation on fluoride profiles was investigated. Layer samples were analyzed by abrasive microsampling. Fluoride and phosphorus concentrations were determined by ion-specific electrode and colorimetric procedures, respectively. There was an increase in fluoride concentrations in the mandible in proportion to the fluoride content in the drinking water and the duration of fluoridation. After fluoridation was discontinued, fluoride concentrations in the surface layers of the mandible presented a decrease. Among the four different parts of the mandible, the upper part of the alveolar bone and the alveolar crest part presented the highest rates of reduction. The relative reduction rate of fluoride concentration was closely related to the duration of discontinuation. The alveolar crest was affected most by the discontinuation of fluoridation, presenting the greatest reduction.

Intestinal obstruction in the terminal ileum caused by an anomalous congenital vascular band between the mesoappendix and the mesentery: report of a case.

We report a case of intestinal obstruction caused by a congenital abnormal vascular band in a 17-year-old boy. The patient was admitted with acute colicky abdominal pain, and an emergency laparotomy revealed that the ileum was strangulated by a fibrous band with vessels about 2 mm in diameter and 7 cm in length, extending from the antemesenterium of the terminal ileum to the mesoappendix. The affected intestine was resected with the band and the appendix. Histologically, the fibrous band was composed of loose connective tissue containing arteries, veins, and nerve fibers, suggesting that it was congenital and originated from a remnant of the ventral mesentery in the embryonic period. There have been few reports of intestinal obstruction being caused by a congenital vascular band, especially in patients beyond the pediatric age group.

[Effect of professional oral prophylaxis on the general health perceptions and lifestyles in workers of a worksite].

The present study was undertaken to look at the effect of professional oral prophylaxis on the general health perceptions and lifestyle in workers at a worksite in Aichi-ken. Dental check-ups and three kinds of questionnaires (SAS: The Social Adjustment Scale, MOS: The Medical Outcomes Study and the nutrition) were used in this investigation for three years from 1998. By means of the stratified sampling method, the participants were 265 workers separated into a case and a control group, who work for a pharmaceutical company (201 males, 64 females, average age 35.6 +/- 0.7 yr old). All participants gave their consent to the study. As in the case group, dental hygienists scaled their teeth and gave each of them appropriate advice about their oral health twice in a year. MOS, SAS, nutrition and oral health in the case and control groups were compared with the baseline, third and the final year. The results were as follows; 1) There was significant difference in energy, protein, fat, fish and meats sufficiency rates (p < 0.05) and a higher tendency towards consuming sugar, potassium (K), calcium (Ca), iron (Fe) and Fiber (p < 0.10) in the control group, but there were no significant differences in the other nutrients in the case and control groups. 2) There were significant differences in the number of decayed teeth in the case group, and in the number of filled teeth and decayed teeth in the control group. The gingival condition (CPI = 0) in the case group tended to be higher healthier than the control group, 3) In the MOS questionnaire, the case group was better or not worse in health perceptions scales, pain scales and mental health scales than the control group. 4) In the SAS questionnaire, the case group was better or not worse in the work and the social scales than the control group. It was concluded that oral health, social health and consciousness of health tended to improve by dental intervention.

Oral pre-cancer and the associated risk factors among industrial workers in Japan's overseas enterprises in the UK.

BACKGROUND: Screening at industries has been advocated as a method of early detection for cancer. This study describes the prevalence of oral pre-cancerous lesions and other mucosal diseases following oral mucosal screening, and associated risk factors among Japanese industrial workers in the UK. METHODS: Oral mucosal screening was by invitation at 51 industrial locations in the UK. A self-administered questionnaire was used to record socio-behavioural factors and frequency of daily intake of fruits and vegetables. RESULTS: Four hundred and eighty-four subjects attended for oral mucosal screening (mean age 39.9 +/- 8.3 years) and their mean period of residence in UK was 5.3 +/- 4.5 years. 63.4% examined were male. 31.3% of males and 26.6% of females smoked daily. The gender differences were striking compared with Japan's national rates. A higher proportion of managerial staff was regular heavy (20+ per day) smokers. The intake of more than five portions per day of vegetables and/or fruits during the weekend was significantly higher in females than in males (P = 0.022). One hundred and six subjects (22%) were detected with oral mucosal lesions, including 16 leukoplakia lesions (3.3%) and three with oral lichen planus (1%). The rate of positive detections was higher in managers (7.5%). Odds ratios were estimated by socio-behavioural variables. Among subjects positive for oral leukoplakia, managers accounted for 68.8% (OR 5.26; 95% CI, 1.24-22.29). 87.5% of subjects detected with oral leukoplakia smoked daily and had done so for the past 10 years (OR 28.40; 95% CI, 5.63-143.28). Though regular alcohol drinking was a common feature among male leukoplakia cases, heavy alcohol misuse was not encountered. None reported an intake of five or more portions of fruits or vegetables. CONCLUSIONS: The Japanese nationals working in managerial positions in the UK and daily regular smokers in the industries visited were found to be at a high risk of oral pre-cancer. Regular dental/oral check up and tobacco education programmes are encouraged for oral cancer/pre-cancer control in industrial settings.

Blood test values and Community Periodontal Index scores in medical checkup recipients.

BACKGROUND: We examined the blood test values of people who received general medical checkups and their Community Periodontal Index (CPI) score. METHODS: A total of 7,452 persons (5,742 males and 1,710 females), who had general medical and dental checkups, were the subjects of the study. Many were people who worked for companies in and around Nagoya and their family members, ranging in age from 16 to 80 years. The blood test in our study consisted of 37 items used in general blood tests. Partial-mouth recordings were used to measure CPI scores. The highest CPI score for each subject was used for analysis. Odds ratios and confidence interval values were obtained using the Mantel-Haenszel method to analyze the results. RESULTS: CPI scores of 3 and 4 were related to the test values of high-density-lipoprotein cholesterol, serum iron, white blood cell count, fasting blood sugar, glycosylated hemoglobin A1, glycosylated hemoglobin A1c, and C-reactive protein. CONCLUSION: Blood test values tended to show correlations with CPI scores, more clearly seen in males than in females.

Der f 16: a novel gelsolin-related molecule identified as an allergen from the house dust mite, Dermatophagoides farinae.

Allergen from the house dust mite (Dermatophagoides sp.) is a major trigger factor of allergic disorders, and its characterization is crucial for the development of specific diagnosis or immunotherapy. Here we report the identification of a novel dust mite (Dermatophagoides farinae) antigen whose primary structure belongs to the gelsolin family, a group of actin cytoskeleton-regulatory proteins. Isolated mite cDNA, termed Der f 16, encodes 480 amino acids comprising a four-repeated gelsolin-like segmental structure, which is not seen in conventional gelsolin family members. Enzyme immunoassay indicated that recombinant Der f 16 protein, prepared using an Escherichia coli expression system, bound IgE from mite-allergic patients at 47% (8/17) frequency. This is the first evidence that the gelsolin family represents a new class of allergen recognizable by atopic patient IgE.

Toward elucidating the full spectrum of mite allergens--state of the art.

Our research has focused on the molecular design of immunotherapeutic vaccines and the advancement of mite-allergy diagnosis. Here, we describe the research history of the major group 1 and group 2 allergens, immunoelectrophoretic analyses covering the complete spectrum of mite allergens, our results on allergens with distinctive characteristics (a conjunctival congestion-eliciting antigen [LM2], an immunotherapeutic antigen [HM2] with high efficacy and without definite adverse reactions, and a potent T-cell stimulatory antigen [HM1] with secretion of IFN-gamma), the full spectrum and immunochemical properties of the major and other important mite allergens (including our newly described allergens: a pan-allergen [tropomyosin, group 10], a potent T-cell stimulatory allergen [M-177, apolipophorin, group 14] and its peptide fragments Mag1 and Mag3, a moderate IgE-binding allergen [gelsolin/villin, group 16], an EF-hand Ca2+-binding allergen [group 17], and a less IgE-binding allergen [heat shock protein 70]), and prospects for the development of immunotherapeutic and diagnostic agents.