ABSTRACT
The effect of fetal neural tissue (FNT) grafting on regeneration of hippocampus structure has been investigated in postischemic rats. Transient global cerebral ischemia was induced by 20-min four-vessel occlusion in 13.2 +/- 2.4-month-old rats. FNT suspension was prepared from hippocampal CA1 area and primordial dentate gyrus of E18-E19 rat fetuses. 30 days after TGI, FNT was stereotactically transplanted into CA1 area of ischemic animals. Linear density of CA1 pyramidal neurons, stratum radiatum width, CA4 and dentate gyrus morphology were studied in hippocampal slices by light microscopy 2, 4 and 7 months after TGI and 1, 3 and 6 months after FNT grafting. It has been shown, that late FNT grafting provides significant and prolonged potentiation of a hippocampal cytoarchitecture recovery after TGI in rats.
Subject(s)
Brain Tissue Transplantation/methods , CA1 Region, Hippocampal/ultrastructure , Fetal Tissue Transplantation/methods , Ischemic Attack, Transient/surgery , Animals , CA1 Region, Hippocampal/embryology , CA1 Region, Hippocampal/transplantation , Disease Models, Animal , Ischemic Attack, Transient/pathology , Rats , Time Factors , Treatment OutcomeABSTRACT
The migration, integration and differentiation of fetal neural progenitor cells (NPCs) in the ischemic brain have been studied. In our study the ischemic insult in FVB line mice was produced by occlusion of both carotid arteries during 20 min. A day after occlusion NPCs from GFP-transgenic 12.5 dpc embryos were suboccipitally transplanted to the ischemic brain. The migration and differentiation of GFP-positive NPCs in the recipient tissue were observed in different time points after their transplantation by immunohistochemical approaches using confocal scanning microscopy. It has been shown that GFP-positive NPCs survived, migrated and differentiated to the mature neurons and glial cells in CA1 area of hippocampus of ischemic animals.
Subject(s)
Brain Ischemia/therapy , Cell Differentiation/physiology , Cell Movement/physiology , Fetal Stem Cells/transplantation , Neurons/transplantation , Stem Cell Transplantation , Animals , Disease Models, Animal , Fetal Stem Cells/cytology , Green Fluorescent Proteins , Immunohistochemistry , Mice , Mice, Inbred Strains , Microscopy, Confocal , Neurons/cytologyABSTRACT
The neuroprotective action by water-soluble form of quercetin was examined in gerbils after transient forebrain ischemia. The animals were exposed to 7 min of bilateral common carotid artery occlusion. Hippocampal CA 1 area was examined 7 days after ischemia-reperfusion. The average density of CA1 pyramidal neurons and GFAP-positive glial cells were counted in sham operated group, in ischemic group and in the groups treated with water-soluble form of quercetin. It was shown that quercetin revealed protective effect by decreasing of delayed neuronal death and reducing reactive astrogliosis after ischemia-reperfusion.
