A review of harmonization strategies for quantitative PET.

PET can reveal in vivo biological processes at the molecular level. PET-derived quantitative values have been used as a surrogate marker for clinical decision-making in numerous clinical studies and trials. However, quantitative values in PET are variable depending on technical, biological, and physical factors. The variability may have a significant impact on a study outcome. Appropriate scanner calibration and quality control, standardization of imaging protocols, and any necessary harmonization strategies are essential to make use of PET as a biomarker with low bias and variability. This review summarizes benefits, limitations, and remaining challenges for harmonization of quantitative PET, including whole-body PET in oncology, brain PET in neurology, PET/MR, and non-18F PET imaging. This review is expected to facilitate harmonization of quantitative PET and to promote the contribution of PET-derived biomarkers to research and development in medicine.

Continuous bed motion in a silicon photomultiplier-based scanner provides equivalent spatial resolution and image quality in whole body PET images at similar acquisition times using the step-and-shoot method.

This study investigated the spatial resolution and image quality of the continuous bed motion (CBM) method in a sensitive silicon photomultiplier (SiPM)-based positron emission tomography (PET)/computed tomography (CT) system compared with the traditional step-and-shoot (SS) method. Methods: Siemens Biograph Vision was used in this study. Data acquisition using the SS method was performed for 3 min per bed. In the CBM method, the bed speed ranged from 0.5 to 3.3 mm/s. The acquisition time equivalent to the SS method was 1.1 mm/s for 2-bed ranges and 0.8 mm/s for seven-bed ranges. The spatial resolution was investigated using 18F point sources and evaluated using the full width at half maximum. Image quality was investigated using a National Electrical Manufacturers Association International Electrotechnical Commission body phantom with six spheres 10-, 13-, 17-, 22-, 28-, and 37-mm inner diameters. The radioactivity concentration ratio of the 18F solution in all spheres and the background was approximately 4:1. The detectability of each sphere was visually evaluated on a five-step score. Image quality was physically evaluated using the noise equivalent count rate (NECphantom), contrast percentage of the 10-mm hot sphere (QH,10mm), background variability percentage (N10mm), and contrast-noise ratio (QH,10mm/N10mm). Results: The spatial resolution was not affected by the difference of acquisition methods and bed speeds. The detectability of the 10-mm sphere with a bed speed of 2.2 mm/s or faster was significantly inferior to that of the SS 2-bed method. In evaluating image quality, no significant difference in the contrast percentage was observed among the acquisition methods and speeds in the CBM method. However, the increasing bed speed in the CBM method increased the N10mm and decreased the NECphantom. When comparing the SS 2-bed method with the CBM method at 0.8 mm/s, no significant differences in all parameters were observed. Conclusion: In a SiPM-based PET/CT scanner, the CBM method provides equivalent spatial resolution and image quality in whole body PET images with same acquisition time using the SS method.

Five-year post-disaster mental changes: Mothers and children living in low-dose contaminated Fukushima regions.

It has been almost 10 years since the accident at Tokyo Electric Power Co., Inc.'s Fukushima Daiichi Nuclear Power Plant in March 2011. This study elucidates changes in the mental states of mothers and children residing in low-dose radiation contaminated regions within Fukushima Prefecture over a five-year period after the Fukushima Daiichi accident. From 2011 to 2015, questionnaire surveys assessing psychological symptoms, including posttraumatic stress disorder-related responses, depressive responses, and stress responses, and radiation protection behaviors were conducted with 18,741 mothers of children aged four, 18, and 42 months. Mothers' and children's psychological symptoms and mothers' radiation protection behaviors were highest in 2011, immediately following the nuclear accident, but decreased over time. However, even in 2015, psychological symptoms and radiation protection behaviors were higher for children and mothers within Fukushima Prefecture than for those in a control group living in regions outside the area, which were minimally affected by the accident. The results suggest that the psychological effects in mothers and children living in low-dose radiation contaminated areas continued for at least five years after the accident. Furthermore, psychological effects in children born after the incident were likely to have been triggered by the parental behavior of mothers who were negatively affected by anxiety and stress. This finding raises concerns regarding the accident's long-lasting psychological effects in mothers and children living in low-contamination regions.

Evaluating and comparing the image quality and quantification accuracy of SiPM-PET/CT and PMT-PET/CT.

OBJECTIVE: The aim of this study was to evaluate the image quality and the quantification accuracy of Biograph Vision PET/CT scanner as a SiPM-PET in comparison to the conventional PMT-PET, Biograph mCT PET/CT scanner. METHODS: This study consisted of a phantom study and a retrospective clinical analysis where patients underwent 18F-FDG PET/CT in both PET systems. The body phantom of the NEMA IEC with 10-37 mm diameter spheres were filled with an 18F-FDG solution. The root mean square error (RMSE) of SUV, the detectability of 10-mm sphere, NECphantom, the background variability (N10mm) and the contrast-noise-ratio (QH,10 mm/N10mm) were calculated based on the phantom analysis. We also examined the quality of the acquired clinical images using the NECpatient, NECdensity, SNRliver, SUVliver and SUVlesion. RESULTS: In the phantom study on Vision scanner, RMSE was relatively lower when the iteration number was 2, 3 or 4. To satisfy a visual score of 1.5 and the reference range of QH,10 mm/N10mm, a 60-s or longer acquisition was required. Our clinical findings show that NECpatient averaged 17.4 ± 1.72 Mcounts/m in mCT and 29.1 ± 2.83 Mcounts/m in Vision. Furthermore, NECdensity averaged 0.29 ± 0.05 kcounts/cm3 in mCT and 0.53 ± 0.09 kcounts/cm3 in Vision, respectively, whereas SNRliver averaged 14.6 ± 3.77% in mCT and 21.3 ± 1.69% in Vision (P = 0.0156), respectively. Finally, SUVliver averaged 2.82 ± 0.28 and 2.55 ± 0.30, SUVlesion ranged 1.6-17.6 and 1.9-22.9 in mCT and Vision, respectively. CONCLUSION: SiPM-PET/CT provides superior image quality and quantification accuracy compared to PMT-PET/CT.

[Evaluation of the Number of Sampling Angles in Three Dimensional Ordered Subset Expectation Maximization: Comparison of Step and Shoot Acquisition and Continuous Acquisition].

Three dimensional ordered subset expectation maximization (3D-OSEM) improves spatial resolution and contrast. Continuous acquisition, and step and shoot acquisition are used in single photon emission computed tomography (SPECT). The purpose of this study was to evaluate the effect of 3D-OSEM when acquisition method was different. We evaluated spatial resolution using a line source phantom and uniformity using a pool phantom. The phantoms were acquired by step and shoot acquisition and continuous acquisition at changing step angles. These projection data were reconstructed using filtered back projection (FBP) and 3D-OSEM. We evaluated reconstruction images using the full width half maximum (FWHM) of line source and root mean square uncertainty (%RMSU) of pool phantom. 3D-OSEM improved spatial resolution and uniformity compared with FBP. Change of FWHM in radial direction and %RMSU by using 3D-OSEM was approximately equal to continuous acquisition in step and shoot acquisition. However, even if using 3D-OSEM, distance between center of rotation and the location of line source is long, a large sampling step angle produced an increase FWHM in tangential direction using continuous acquisition. Step angles need to be set based on the sampling theorem using continuous acquisition.

The Influence of Minimal Misalignment on the Repeatability of PET Images Examined by the Repositioning of Point Sources.

We aimed to evaluate the influence of minimal misalignment of a hot spot on the repeatability of PET images using repositioning of point sources. Methods: Point sources with an inner diameter of 1 mm were made with 1 µL of 18F solution. Seven point sources were placed on the x-axis in the field of view. For fixed-position imaging, PET data were acquired for 10 min 5 times serially. For variable-position imaging, PET data were acquired for 10 min each with the point sources placed at 0, ±0.5, and ±1.0 mm in the x-axis direction. The data were reconstructed using ordered-subsets expectation maximization (OSEM) and OSEM plus point-spread function (PSF). The image matrix was 128 × 128, 200 × 200, 256 × 256, 400 × 400, and 512 × 512 pixels. The normalized maximum count (rMax), the coefficient of variance (CVmax), and the full width at half maximum were analyzed. Results: The hot spots on OSEM images far from the center became faint and broad, whereas those on OSEM+PSF images became small and dense. Although rMax was overestimated at the 5-cm position on OSEM images, rMax at other positions was overestimated on OSEM+PSF images with a matrix of at least 256 × 256. rMax showed a similar pattern in fixed- and variable-position images. CVmax in fixed-position OSEM images was less than 2%, irrespective of matrix size. In contrast, CVmax in variable-position images was higher than in fixed-position images. CVmax was higher for OSEM+PSF images than for OSEM images. The full width at half maximum increased at positions far from the center on OSEM images but was stable at all positions on OSEM+PSF images. Conclusion: The repeatability of the small hot spot was affected by the minimal misalignment, especially on OSEM+PSF images. Precise positioning is necessary if PET is to be used as a biomarker. Professionals should recognize that PSF correction worsens the repeatability of the small hot spot although improving the spatial resolution of PET images.

Multicentre analysis of PET SUV using vendor-neutral software: the Japanese Harmonization Technology (J-Hart) study.

BACKGROUND: Recent developments in hardware and software for PET technologies have resulted in wide variations in basic performance. Multicentre studies require a standard imaging protocol and SUV harmonization to reduce inter- and intra-scanner variability in the SUV. The Japanese standardised uptake value (SUV) Harmonization Technology (J-Hart) study aimed to determine the applicability of vendor-neutral software on the SUV derived from positron emission tomography (PET) images. The effects of SUV harmonization were evaluated based on the reproducibility of several scanners and the repeatability of an individual scanner. Images were acquired from 12 PET scanners at nine institutions. PET images were acquired over a period of 30 min from a National Electrical Manufacturers Association (NEMA) International Electrotechnical Commission (IEC) body phantom containing six spheres of different diameters and an 18F solution with a background activity of 2.65 kBq/mL and a sphere-to-background ratio of 4. The images were reconstructed to determine parameters for harmonization and to evaluate reproducibility. PET images with 2-min acquisition × 15 contiguous frames were reconstructed to evaluate repeatability. Various Gaussian filters (GFs) with full-width at half maximum (FWHM) values ranging from 1 to 15 mm in 1-mm increments were also applied using vendor-neutral software. The SUVmax of spheres was compared with the reference range proposed by the Japanese Society of Nuclear Medicine (JSNM) and the digital reference object (DRO) of the NEMA phantom. The coefficient of variation (CV) of the SUVmax determined using 12 PET scanners (CVrepro) was measured to evaluate reproducibility. The CV of the SUVmax determined from 15 frames (CVrepeat) per PET scanner was measured to determine repeatability. RESULTS: Three PET scanners did not require an additional GF for harmonization, whereas the other nine required additional FWHM values of GF ranging from 5 to 9 mm. The pre- and post-harmonization CVrepro of six spheres were (means ± SD) 9.45% ± 4.69% (range, 3.83-15.3%) and 6.05% ± 3.61% (range, 2.30-10.7%), respectively. Harmonization significantly improved reproducibility of PET SUVmax (P = 0.0055). The pre- and post-harmonization CVrepeat of nine scanners were (means ± SD) 6.59% ± 1.29% (range, 5.00-8.98%) and 4.88% ± 1.64% (range, 2.65-6.72%), respectively. Harmonization also significantly improved the repeatability of PET SUVmax (P < 0.0001). CONCLUSIONS: Harmonizing SUV using vendor-neutral software produced SUVmax for 12 scanners that fell within the JSNM reference range of a NEMA body phantom and improved SUVmax reproducibility and repeatability.

Time-of-Flight Information Improved the Detectability of Subcentimeter Spheres Using a Clinical PET/CT Scanner.

Recent advancements in clinical PET/CT scanners have improved the detectability of small lesions. However, the ideal reconstruction parameters for detecting small lesions have not yet been sufficiently clarified. The purpose of this study was to investigate the detectability of subcentimeter spheres using a clinical PET/CT scanner. Methods: We used a clinical PET/CT scanner to obtain the data of a National Electrical Manufacturers Association body phantom consisting of 6 small spheres (inner diameters, 4.0, 5.0, 6.2, 7.9, 10, and 37 mm) containing 18F solution. The background activity was 2.65 kBq/mL, and the sphere-to-background ratio was 8. The PET data obtained for 2 and 120 min were reconstructed using ordered-subsets expectation maximization (OSEM), OSEM + point-spread function (PSF), and OSEM + time-of-flight (TOF) with voxel sizes of 2.04 × 2.04 × 2.00 mm (2-mm voxels) and 4.07 × 4.07 × 3.99 mm (4-mm voxels). A gaussian filter was not used. The image quality was evaluated by visual assessment, as well as by physical assessment of the detectability index and recovery coefficients. Results: According to the visual assessment, the detectability of the spheres improved using TOF and a longer acquisition. Using the OSEM+TOF model, the smallest visually detected spheres were 5 mm in diameter with a 120-min acquisition and 6 mm in diameter with a 2-min acquisition. According to physical assessment, the detectability of spheres 10 mm or smaller using the OSEM+TOF image was superior to that using the OSEM image. In addition, the detectability of each hot sphere and recovery coefficient with 2-mm voxels was superior to that with 4-mm voxels. Although OSEM+PSF images showed less background noise, detectability and the recovery coefficient were not improved for spheres 8 mm or smaller. Conclusion: The TOF model with 2-mm voxels improved the detectability of subcentimeter hot spheres on a clinical PET/CT scanner.

Characteristics of Smoothing Filters to Achieve the Guideline Recommended Positron Emission Tomography Image without Harmonization.

OBJECTIVES: The aim of this study is to examine the effect of different smoothing filters on the image quality and SUVmax to achieve the guideline recommended positron emission tomography (PET) image without harmonization. METHODS: We used a Biograph mCT PET scanner. A National Electrical Manufacturers Association (NEMA) the International Electrotechnical Commission (IEC) body phantom was filled with 18F solution with a background activity of 2.65 kBq/mL and a sphere-to-background ratio of 4. PET images obtained with the Biograph mCT PET scanner were reconstructed using the ordered subsets-expectation maximization (OSEM) algorithm with time-of-flight (TOF) models (iteration, 2; subset, 21); smoothing filters including the Gaussian, Butterworth, Hamming, Hann, Parzen, and Shepp-Logan filters with various full width at half maximum (FWHM) values (1-15 mm) were applied. The image quality was physically assessed according to the percent contrast (QH,10), background variability (N10), standardized uptake value (SUV), and recovery coefficient (RC). The results were compared with the guideline recommended range proposed by the Japanese Society of Nuclear Medicine and the Japanese Society of Nuclear Medicine Technology. The PET digital phantom was developed from the digital reference object (DRO) of the NEMA IEC body phantom smoothed using a Gaussian filter with a 10-mm FWHM and defined as the reference image. The difference in the SUV between the PET image and the reference image was evaluated according to the root mean squared error (RMSE). RESULTS: The FWHMs of the Gaussian, Butterworth, Hamming, Hann, Parzen, and Shepp-Logan filters that satisfied the image quality of the FDG-PET/CT standardization guideline criteria were 8-12 mm, 9-11 mm, 9-13 mm, 10-13 mm, 9-11 mm, and 12-15 mm, respectively. The FWHMs of the Gaussian, Butterworth, Hamming, Hann, Parzen, and Shepp-Logan filters that provided the smallest RMSE between the PET images and the 3D digital phantom were 7 mm, 8 mm, 8 mm, 8 mm, 7 mm, and 11 mm, respectively. CONCLUSION: The suitable FWHM for image quality or SUVmax depends on the type of smoothing filter that is applied.

Edge Artifacts in Point Spread Function-based PET Reconstruction in Relation to Object Size and Reconstruction Parameters.

OBJECTIVES: We evaluated edge artifacts in relation to phantom diameter and reconstruction parameters in point spread function (PSF)-based positron emission tomography (PET) image reconstruction. METHODS: PET data were acquired from an original cone-shaped phantom filled with 18F solution (21.9 kBq/mL) for 10 min using a Biograph mCT scanner. The images were reconstructed using the baseline ordered subsets expectation maximization (OSEM) algorithm and the OSEM with PSF correction model. The reconstruction parameters included a pixel size of 1.0, 2.0, or 3.0 mm, 1-12 iterations, 24 subsets, and a full width at half maximum (FWHM) of the post-filter Gaussian filter of 1.0, 2.0, or 3.0 mm. We compared both the maximum recovery coefficient (RCmax) and the mean recovery coefficient (RCmean) in the phantom at different diameters. RESULTS: The OSEM images had no edge artifacts, but the OSEM with PSF images had a dense edge delineating the hot phantom at diameters 10 mm or more and a dense spot at the center at diameters of 8 mm or less. The dense edge was clearly observed on images with a small pixel size, a Gaussian filter with a small FWHM, and a high number of iterations. At a phantom diameter of 6-7 mm, the RCmax for the OSEM and OSEM with PSF images was 60% and 140%, respectively (pixel size: 1.0 mm; FWHM of the Gaussian filter: 2.0 mm; iterations: 2). The RCmean of the OSEM with PSF images did not exceed 100%. CONCLUSION: PSF-based image reconstruction resulted in edge artifacts, the degree of which depends on the pixel size, number of iterations, FWHM of the Gaussian filter, and object size.

Estimation of ambient dose equivalent distribution in the 18F-FDG administration room using Monte Carlo simulation.

In this study, we estimated the ambient dose equivalent rate (hereafter "dose rate") in the fluoro-2-deoxy-D-glucose (FDG) administration room in our hospital using Monte Carlo simulations, and examined the appropriate medical-personnel locations and a shielding method to reduce the dose rate during FDG injection using a lead glass shield. The line source was assumed to be the FDG feed tube and the patient a cube source. The dose rate distribution was calculated with a composite source that combines the line and cube sources. The dose rate distribution was also calculated when a lead glass shield was placed in the rear section of the lead-acrylic shield. The dose rate behind the automatic administration device decreased by 87 % with respect to that behind the lead-acrylic shield. Upon positioning a 2.8-cm-thick lead glass shield, the dose rate behind the lead-acrylic shield decreased by 67 %.

The influence of respiratory motion on the cumulative SUV-volume histogram and fractal analyses of intratumoral heterogeneity in PET/CT imaging.

OBJECTIVE: The purpose of this study was to investigate the influence of respiratory motion on the evaluation of the intratumoral heterogeneity of FDG uptake using cumulative SUV-volume histogram (CSH) and fractal analyses. METHODS: We used an NEMA IEC body phantom with a homogeneous hot sphere phantom (HO) and two heterogeneous hot sphere phantoms (HE1 and HE2). The background radioactivity of (18)F in the NEMA phantom was 5.3 kBq/mL. The ratio of radioactivity was 4:2:1 for the HO and the outer rims of the HE1 and HE2 phantoms, the inner cores of the HE1 and HE2 phantoms, and background, respectively. Respiratory motion was simulated using a motion table with an amplitude of 2 cm. PET/CT data were acquired using Biograph mCT in motionless and moving conditions. The PET images were analyzed by both CSH and fractal analyses. The area under the CSH (AUC-CSH) and the fractal dimension (FD) was used as quantitative metrics. RESULTS: In motionless conditions, the AUC-CSHs of the HO (0.80), HE1 (0.75) and HE2 (0.65) phantoms were different. They did not differ in moving conditions (HO, 0.63; HE1, 0.65; HE2, 0.60). The FD of the HO phantom (0.77) was smaller than the FDs of the HE1 (1.71) and HE2 (1.98) phantoms in motionless conditions; however, the FDs of the HO (1.99) and HE1 (2.19) phantoms were not different from each other and were smaller than that of the HE2 (3.73) phantom in moving conditions. CONCLUSION: Respiratory motion affected the results of the CSH and fractal analyses for the evaluation of the heterogeneity of the PET/CT images. The influence of respiratory motion was considered to vary depending on the object size.

Relationship between the image quality and noise-equivalent count in time-of-flight positron emission tomography.

OBJECTIVE: The aim of this study was to investigate the relationship between the NEC and TOF-PET image quality. METHODS: The National Electrical Manufactures Association and International Electrical Commission (NEMA IEC) body phantom with a 10-mm diameter sphere was filled with an 18F-FDG solution with a 4:1 radioactivity ratio. The PET data were acquired in the three-dimensional list mode for 20 min. We created frame data ranging from 1 to 5 min acquisition time, which were then reconstructed using the baseline ordered-subsets expectation maximization (OSEM), the OSEM + point spread function (PSF) algorithm, OSEM + time-of-flight (TOF) algorithm and OSEM + PSF + TOF algorithm. The PET images were analyzed according to the noise-equivalent count (NEC), the coefficients of variance of the background (CVBG), the maximum count (CVmax) and the contrast (CVCONT). The results were compared with the recommended value according to the guidelines for the oncology FDG-PET/CT protocol. RESULTS: The NEC was higher than the recommended value at 3 min or longer acquisition time. The CVBG lower than 15% were obtained at 3 min acquisition time without TOF and at 2 min acquisition time with TOF. The CVBG of 10% or lower were obtained at 5 min or longer acquisition time without TOF and at 4 min or longer acquisition time with TOF. Both the CVmax and CVCONT lower than 10% were obtained at 3 min or longer acquisition time without TOF and at 1 min acquisition or longer with TOF. No particular relationships were observed between the frame number and degree of the variation in the image quality. The CVCONT significantly correlated with the NEC for the data reconstructed without TOF information, while there were no significant correlations between these useful metrics for the data reconstructed with TOF. CONCLUSION: This study demonstrated that the NEC is not a useful metric for the evaluation of the image quality on TOF-PET images.

The edge artifact in the point-spread function-based PET reconstruction at different sphere-to-background ratios of radioactivity.

OBJECTIVE: The aim of this study was to quantitatively evaluate the edge artifacts in PET images reconstructed using the point-spread function (PSF) algorithm at different sphere-to-background ratios of radioactivity (SBRs). METHODS: We used a NEMA IEC body phantom consisting of six spheres with 37, 28, 22, 17, 13 and 10 mm in inner diameter. The background was filled with (18)F solution with a radioactivity concentration of 2.65 kBq/mL. We prepared three sets of phantoms with SBRs of 16, 8, 4 and 2. The PET data were acquired for 20 min using a Biograph mCT scanner. The images were reconstructed with the baseline ordered subsets expectation maximization (OSEM) algorithm, and with the OSEM + PSF correction model (PSF). For the image reconstruction, the number of iterations ranged from one to 10. The phantom PET image analyses were performed by a visual assessment of the PET images and profiles, a contrast recovery coefficient (CRC), which is the ratio of SBR in the images to the true SBR, and the percent change in the maximum count between the OSEM and PSF images (Δ % counts). RESULTS: In the PSF images, the spheres with a diameter of 17 mm or larger were surrounded by a dense edge in comparison with the OSEM images. In the spheres with a diameter of 22 mm or smaller, an overshoot appeared in the center of the spheres as a sharp peak in the PSF images in low SBR. These edge artifacts were clearly observed in relation to the increase of the SBR. The overestimation of the CRC was observed in 13 mm spheres in the PSF images. In the spheres with a diameter of 17 mm or smaller, the Δ % counts increased with an increasing SBR. The Δ % counts increased to 91 % in the 10-mm sphere at the SBR of 16. CONCLUSIONS: The edge artifacts in the PET images reconstructed using the PSF algorithm increased with an increasing SBR. In the small spheres, the edge artifact was observed as a sharp peak at the center of spheres and could result in overestimation.

Differential roles of dopamine D1 and D2 receptor-containing neurons of the nucleus accumbens shell in behavioral sensitization.

The nucleus accumbens (Nac) mediates the reinforcing and motor stimulating properties of psychostimulants. It receives dopaminergic afferents from the ventral midbrain and is divided into two distinct subregions: shell and core. Each of these contains two subtypes of medium spiny neurons, which express either dopamine D1 (D1R) or D2 (D2R) receptors. However, functional dissociation between the two subtypes in psychostimulant response remains to be elucidated. We performed selective ablation of each subtype in the Nac shell in mice, using immunotoxin-mediated cell targeting, and examined the behavioral sensitization evoked by repeated administration of methamphetamine. The D1R cell-ablated mice exhibited delayed induction of sensitized locomotion compared to control mice, whereas the D2R cell-ablated mice showed a mildly enhanced rate of induction of sensitization. In vivo microdialysis revealed a marked blockade of the increase in extracellular dopamine in the Nac of the D1R cell-ablated animals in response to methamphetamine, indicating that the observed delay in behavioral sensitization in these mice involves an impairment in accumbal dopamine release. Our results reveal differential roles of D1R- and D2R-containing accumbal shell neurons in the development of behavioral sensitization to psychostimulants. Behavioral sensitization, enhanced motility by repetitive psychostimulant administration, is a model of drug addiction. Here, we show that the nucleus accumbens (Nac) shell neurons containing dopamine D1 receptor (D1R) or D2 receptor (D2R) play distinct roles in behavioral sensitization triggered by methamphetamine, and that D1R-containing neurons enhance the induction of behavioral sensitization at the early phase, whereas D2R-containing neurons act to suppress the rate of development of the behavior.

Improvement in PET/CT image quality in overweight patients with PSF and TOF.

OBJECTIVE: The aim of this study was to evaluate the effect of the point spread function (PSF) and time of flight (TOF) on PET/CT images of overweight patients in relation to the iteration number and the acquisition time. METHODS: This study consisted of a phantom study and a clinical study. The NEMA IEC body phantom and a 40 cm diameter large phantom (LG phantom) simulating an overweight patient were used in this study. Both phantoms were filled with (18)F solution with a sphere to background ratio of 4:1. The PET data were reconstructed with the baseline ordered-subsets expectation maximization (OSEM) algorithm, with the OSEM + PSF model, with the OSEM + TOF model and with the OSEM + PSF + TOF model. The clinical study was a retrospective analysis of 66 patients who underwent (18)F-FDG PET/CT. The image quality was evaluated using the background variability (coefficient of variance, CVphantom and CVliver) and the contrast (CONTHOT and SNR). RESULTS: In phantom study, the CVphantom of the LG phantom was higher than that of the NEMA phantom. The PSF decreased the CVphantom of the LG phantom to the NEMA phantom level. The TOF information accelerated the CVphantom plateau earlier. The best relationship between the CVphantom and the CONTHOT was observed for the OSEM + PSF + TOF. In clinical study, the combination of PSF and TOF decreased the CVliver for overweight patients to that for normal weight patients while it increased the SNR similarly between two patient groups. CONCLUSION: The combination of the PSF and TOF correction improved the image quality of the LG phantom and overweight patients.

Lesions of the nucleus accumbens core modulate development of matching behavior.

BACKGROUND: The development of choice is a crucial determinant in the performance of appetitive responses. Given two options with different reinforcement rates, animals match their relative rate of responding to the relative rates of reinforcement (i.e., matching behavior). A previous study has shown that the nucleus accumbens core (AcbC) is involved in the performance of matching behavior in trained animals. However, the role of the AcbC in the acquisition of matching behavior has not been addressed. RESULTS: We conducted a series of experimental sessions to examine the role of the AcbC on the development of matching behavior. Instrumental responding was measured in rats with excitotoxic lesions of the AcbC. Rats were given two options that differed in the relative rate of reinforcement under concurrent variable-interval schedules. The locations of the more frequently reinforced option and the alternative option were randomly switched between sessions. Lesions of the AcbC accelerated the development of matching behavior compared to the sham-operated group. The AcbC-lesioned rats exhibited closer conformity to the matching law than shams when the options were in the same positions as in the previous session (the same condition), but not when the option locations had been switched (the different condition). The AcbC rats showed smaller probabilities of switching behavior between alternatives than shams. Post-reinforcement pausing was not affected by the AcbC lesion. Neither numbers of rewards obtained nor number of lever presses were different between the AcbC-lesioned rats and shams over session blocks. CONCLUSIONS: Our results suggest that the AcbC plays a regulatory role in the development of matching behavior through switching probabilities rather than perception of reward magnitude. The differential effect of AcbC lesions on the matching behavior between the same and different conditions suggests influence of the spontaneous recovery, that is, reversion to a previously reinforced choice at the beginning of the next session, on the development of matching behavior in the AcbC-lesioned rats.

Accuracy of amplitude-based respiratory gating for PET/CT in irregular respirations.

OBJECTIVE: We evaluated the accuracy of amplitude gating PET (AG-PET) compared with phase gating PET (PG-PET) in relation to respiratory motion patterns based on a phantom analysis. METHOD: We used a NEMA IEC body phantom filled with an (18)F solution with a 4:1 sphere-to-background radioactivity ratio (12.6 and 2.97 kBq/mL). PET/CT scans were acquired in a motionless and moving state on a Biograph mCT. The respiratory movements were simulated by four different waveform patterns consisting of ideal breathing, breathing with a pause period, breathing with a variable amplitude and breathing with a changing baseline. AG-PET selects the narrow bandwidth containing 20 % of the respiratory cycle. PG-PET was reconstructed with five gates. The image quality was physically assessed using the percent contrast (Q H,10mm), background variability (N 10mm) recovery coefficient (RC), and sphere volumes. RESULT: In regular motion patterns with ideal breathing and breathing with a pause period, the Q H,10mm, RC and sphere volumes were not different between AG-PET and PG-PET. In the variable amplitude pattern, the Q H,10mm of AG-PET was higher than that of PG-PET (35.8 vs 28.2 %), the RC of AG-PET was higher than that of PG-PET and sphere volume of AG-PET was smaller than that of PG-PET (6.4 vs 8.6 mL). In the changing baseline pattern, the Q H,10mm of AG-PET was higher than that of PG-PET (42.4 vs 16.7 %), the RC of AG-PET was higher than that of PG-PET and sphere volume of AG-PET was smaller than that of PG-PET (6.2 vs 9.8 mL). The N 10mm did not differ between AG-PET and PG-PET, irrespective of the motion pattern. CONCLUSION: Amplitude gating PET is considered to be more accurate than phase gating PET for examining unstable respiratory motion patterns, such as those involving a variable amplitude or changing baseline.