1.
Biull Eksp Biol Med
; 83(5): 551-3, 1977 May.
Article
in Russian
| MEDLINE
| ID: mdl-884257
ABSTRACT
The influence of heparin on the protein fractions and DNA of the isolated nuclei of hepatocytes was studied by biochemical and cytospectrophotometrical methods on Wistar rats. It was found that 0.05% sodium heparinate solution blocked 95% of the histones, not influencing the non-histone proteins of the cell nucleus. Histone blocking was connected with the DNA release into the incubation medium in the amounts proportional to the incubation period.
Subject(s)
DNA/metabolism , Heparin/pharmacology , Liver/drug effects , Animals , Histones/metabolism , Liver/metabolism , Male , Nucleoproteins/metabolism , Rats
2.
Arkh Patol
; 39(6): 71-3, 1977.
Article
in Russian
| MEDLINE
| ID: mdl-71890
ABSTRACT
After hydrolysis with 5N HCl at room temperature Feulgen's reaction either with Schiff's reagent or with some basic dyes instead of it can be carried out on the air-dried smears without any chemical fixation. Both reactions give quantitative results.
Subject(s)
Staining and Labeling/methods , Animals , Chromium , Evaluation Studies as Topic , Gentian Violet , Hydrolysis , Liver/cytology , Oxazines , Rats , Tolonium Chloride
3.
Vrach Delo
; (10): 29-32, 1976 Oct.
Article
in Russian
| MEDLINE
| ID: mdl-1022027