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1.
Retrovirology ; 10: 4, 2013 Jan 10.
Article in English | MEDLINE | ID: mdl-23305456

ABSTRACT

BACKGROUND: Human immunodeficiency virus type 1 (HIV-1) undergoes a protease-mediated maturation process that is required for its infectivity. Little is known about how the physical properties of viral particles change during maturation and how these changes affect the viral lifecycle. Using Atomic Force Microscopy (AFM), we previously discovered that HIV undergoes a "stiffness switch", a dramatic reduction in particle stiffness during maturation that is mediated by the viral Envelope (Env) protein. RESULTS: In this study, we show that transmembrane-anchored Env cytoplasmic tail (CT) domain is sufficient to regulate the particle stiffness of immature HIV-1. Using this construct expressed in trans with viral Env lacking the CT domain, we show that increasing particle stiffness reduces viral entry activity in immature virions. A similar effect was also observed for immature HIV-1 pseudovirions containing Env from vesicular stomatitis virus. CONCLUSIONS: This linkage between particle stiffness and viral entry activity illustrates a novel level of regulation for viral replication, providing the first evidence for a biological role of virion physical properties and suggesting a new inhibitory strategy.


Subject(s)
HIV-1/pathogenicity , Virion/pathogenicity , Virus Internalization , Humans , Microscopy, Atomic Force , Models, Biological , Viral Matrix Proteins/physiology , Virion/chemistry , Virus Replication/physiology , env Gene Products, Human Immunodeficiency Virus/chemistry , gag Gene Products, Human Immunodeficiency Virus/chemistry , gag Gene Products, Human Immunodeficiency Virus/physiology
2.
J Virol Methods ; 169(1): 244-7, 2010 Oct.
Article in English | MEDLINE | ID: mdl-20691213

ABSTRACT

Elucidating the structure of the immature HIV-1 Gag core is an important aspect of understanding the biology of this virus. In doing so, preservation of the fragile Gag lattice is essential. In this study, the effects of purification methods on the structural and mechanical integrity of immature HIV-1 are examined. The results show that the morphological and mechanical properties of the virion are preserved to a significantly higher degree by Iodixanol (OptiPrep) purification compared to the standard sucrose method. In conclusion, these results indicate that OptiPrep instead of sucrose purification should be employed when conducting structural studies on the HIV-1 virion.


Subject(s)
HIV-1/isolation & purification , Ultracentrifugation/methods , Virology/methods , Animals , Cryoelectron Microscopy , HIV-1/chemistry , HIV-1/ultrastructure , Sucrose , Triiodobenzoic Acids , Virion/chemistry , Virion/isolation & purification , Virion/ultrastructure
3.
Biophys J ; 92(5): 1777-83, 2007 Mar 01.
Article in English | MEDLINE | ID: mdl-17158573

ABSTRACT

After budding from the cell, human immunodeficiency virus (HIV) and other retrovirus particles undergo a maturation process that is required for their infectivity. During maturation, HIV particles undergo a significant internal morphological reorganization, changing from a roughly spherically symmetric immature particle with a thick protein shell to a mature particle with a thin protein shell and conical core. However, the physical principles underlying viral particle production, maturation, and entry into cells remain poorly understood. Here, using nanoindentation experiments conducted by an atomic force microscope (AFM), we report the mechanical measurements of HIV particles. We find that immature particles are more than 14-fold stiffer than mature particles and that this large difference is primarily mediated by the HIV envelope cytoplasmic tail domain. Finite element simulation shows that for immature virions the average Young's modulus drops more than eightfold when the cytoplasmic tail domain is deleted (930 vs. 115 MPa). We also find a striking correlation between the softening of viruses during maturation and their ability to enter cells, providing the first evidence, to our knowledge, for a prominent role for virus mechanical properties in the infection process. These results show that HIV regulates its mechanical properties at different stages of its life cycle (i.e., stiff during viral budding versus soft during entry) and that this regulation may be important for efficient infectivity. Our report of this maturation-induced "stiffness switch" in HIV establishes the groundwork for mechanistic studies of how retroviral particles can regulate their mechanical properties to affect biological function.


Subject(s)
HIV/physiology , Biomechanical Phenomena , HIV/ultrastructure , Humans , Microscopy, Atomic Force/methods
4.
Virology ; 360(2): 477-91, 2007 Apr 10.
Article in English | MEDLINE | ID: mdl-17157347

ABSTRACT

Herpes simplex virus type 1 (HSV-1) entry into permissive cells involves attachment to cell-surface glycosaminoglycans (GAGs) and fusion of the virus envelope with the cell membrane triggered by the binding of glycoprotein D (gD) to cognate receptors. In this study, we characterized the observation that soluble forms of the gD ectodomain (sgD) can mediate entry of gD-deficient HSV-1. We examined the efficiency and receptor specificity of this activity and used sequential incubation protocols to determine the order and stability of the initial interactions required for entry. Surprisingly, virus binding to GAGs did not increase the efficiency of sgD-mediated entry and gD-deficient virus was capable of attaching to GAG-deficient cells in the absence of sgD. These observations suggested a novel binding interaction that may play a role in normal HSV infection.


Subject(s)
Herpesvirus 1, Human/physiology , Receptors, Virus/physiology , Viral Envelope Proteins/physiology , Virus Attachment , Virus Internalization , Animals , CHO Cells , Cell Line , Chlorocebus aethiops , Cricetinae , Cricetulus , Gene Deletion , Glycosaminoglycans/deficiency , Herpesvirus 1, Human/genetics , Humans , Receptors, Virus/genetics , Vero Cells , Viral Envelope Proteins/genetics
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