ABSTRACT
High-resolution mass spectrometry and quantum mechanics/molecular mechanics studies were employed for characterizing the formation of two gold finger (GF) domains from the reaction of zinc fingers (ZF) with gold complexes. The influence of both the gold oxidation state and the ZF coordination sphere in GF formation provided useful insights into the possible design of new gold complexes targeting specific ZF motifs.
Subject(s)
Computer Simulation , Coordination Complexes/chemistry , Gold/chemistry , Mass Spectrometry , Zinc Fingers , Magnetic Resonance SpectroscopyABSTRACT
New low-energy electron injection systems based on indirectly heated dispenser cathodes facilitate electron capture dissociation (ECD) in Fourier transform ion cyclotron resonance (FTICR) mass spectrometry. In this joint report, details are presented of the design and performance of these systems on two commercial FTICR instruments, 9.4 T Bruker BioAPEX in Uppsala and 4.7 T IonSpec Ultima in Odense. New results include obtaining meaningful one-scan MS/MS data for isolated precursor ions with millisecond irradiation times. The ECD rate improvement is not only due to the larger total electron current, but the larger emitting area as well.
Subject(s)
Electrons , Mass Spectrometry/instrumentation , Mass Spectrometry/methods , Oligopeptides/chemistry , Peptides/chemistry , Spectrometry, Mass, Electrospray Ionization/methods , Amino Acid Sequence , Cyclotrons , Equipment Design , Fourier Analysis , Sensitivity and Specificity , Spectrometry, Mass, Electrospray Ionization/instrumentationABSTRACT
In order to be able to study complex biological samples, a micro-capillary liquid chromatography system was coupled to a 9.4 T Fourier transform ion cyclotron resonance mass spectrometer. The setup was tested on a tryptic digest of bovine serum albumin, which resulted in high sequence coverage (> 92%) of the protein.
