ABSTRACT
Biological activity of pure extracts of cultural filtrates of Aspergillus niveus 2411, Myrothecium cinctum 910, Ulocladium consortiale 960, Penicillium sp. 10-51 concerning wide spectrum of test-organisms was investigated. It was shown that the extracts had high levels of antibacterial activity against Gram-positive microorganisms, especially against Bacillus genus. But their activity against Gram-negative bacteria was a bit lower. On the other hand, metabolites of M. cinctum 910 and Penicillium sp. 10-51 did show the activity concerning phytopathogenic bacteria. Extracts of fungi showed fungistatic activity against yeasts, but they were not so active concerning fungal test-cultures. Extracts of A. niveus 2411, Penicillium sp. 10-51 suppressed the growth of Phoma betae. The highest level of fungistatic activity was shown by metabolites of M. cinctum 910. They showed activity against Aspergillus genus strains and phytopathogenic isolates of Fusarium lactis, Rhizoctonia solani and Botrytis cinerea.
Subject(s)
Anti-Bacterial Agents/pharmacology , Antifungal Agents/pharmacology , Aspergillus/chemistry , Hypocreales/chemistry , Penicillium/chemistry , Spiroplasma/chemistry , Anti-Bacterial Agents/isolation & purification , Antifungal Agents/isolation & purification , Aspergillus/metabolism , Bacillus/drug effects , Bacillus/growth & development , Botrytis/drug effects , Botrytis/growth & development , Complex Mixtures/chemistry , Fusarium/drug effects , Fusarium/growth & development , Hypocreales/metabolism , Microbial Sensitivity Tests , Penicillium/metabolism , Rhizoctonia/drug effects , Rhizoctonia/growth & development , Spiroplasma/metabolismABSTRACT
Antibiotic activity screening of 15 species of Aspergillus parvulus were made relative to different test-organisms (gram positive, gram negative, phytopathogenic bacteria and yeasts) and phytotoxic activity screening relative to different algae. The researched strains 3387, 340, 3142, 1813 of A. parvulus had a wide spectrum of antibiotic activity and high phytotoxic activity. The rest of species had different degrees of antibiotic and phytotoxic activity. For further investigation the evaporated cultural filtrate of A. parvulus 3142 was separated by the method of column chromatography into fractions with different properties. Minimum suppressing concentration (which equals 2 mg/ml for most active fraction) was determined for the most active one, and a system of solvents was chosen as a results of Schevchic assembly chromatography for the extraction of active substance (n-buthanol saturated water).
