ABSTRACT
A Gram-negative, strictly aerobic, motile, rod-shaped bacterium with monopolar flagellum, designated as F51T, was isolated from the skin ulcer of farmed Murray cod sampled from Zhejiang Province, China. Strain F51T grew at 4-37 °C (optimal temperature, 28 °C), pH 5.0-8.5 (optimal pH, 7.5) and NaCl concentration of 0-6.0% (w/v) (optimal concentration, 2.0%). Phylogenetic analysis based on average nucleotide identity (76.2-78.4%) and in silico DNA-DNA hybridization (22.3-23.2%) values revealed that strain F51T forms a distinct lineage in the clade of genus Pseudomonas with less than 98.9% 16S rRNA gene sequence similarity to type strains of the genus and represents a novel species related most closely to Pseudomonas floridensis LMG 30013T. Three housekeeping genes (rpoB, rpoD and gyrB) of strain F51T were analysed to further confirm that the isolate is distinctly delineated from related Pseudomonas species. Chemotaxonomic analysis indicated that the sole respiratory quinone of strain F51T is Q-9; its predominant cellular fatty acids are C16:0, summed feature 3 (iso-C15:0 2-OH and/or C16:1ω7c), summed feature 8 (C18:1ω7c and/or C18:1ω6c) and C10:0 3-OH; and its major polar lipids consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified glycolipids, three unidentified phospholipids and an unidentified aminophosphoglycolipid. This composition is typical of the chemotaxonomic attributes of Pseudomonas. Based on its phenotypic, chemotaxonomic and phylogenetic features, strain F51T is considered to represent a novel species for which the name Pseudomonas ovata sp. nov. is proposed. The type strain is F51T (= KCTC 62133T = MCCC 1K03458T).
Subject(s)
Fish Diseases/microbiology , Perciformes/microbiology , Phylogeny , Pseudomonas/classification , Pseudomonas/physiology , Animals , Aquaculture , Base Composition , China , DNA, Bacterial/genetics , Genes, Bacterial/genetics , Genome, Bacterial/genetics , Lipids/chemistry , Nucleic Acid Hybridization , Pseudomonas/chemistry , Pseudomonas/genetics , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Species SpecificityABSTRACT
A yellow-pigmented, Gram-staining-negative, aerobic, non-motile and rod-shaped bacterium, designated strain F30T, was isolated from fresh water of a diseased farmed Murray cod with a profound ulceration pond in Zhejiang province, PR China. Growth was observed at NaCl concentrations of 0.5-3.5â% (w/v) (optimum, 1.5-2.0â%), temperatures of 10-35 °C (optimum, 28 °C) and pH 5.0-9.0 (optimum, 6.5). Phylogenetic analysis based on 16S rRNA gene sequences indicated that F30T represented a member of the genus Chryseobacterium, showing the highest similarity to Chryseobacterium jejuense DSM 19299T (99.0â%) and Chryseobacterium nakagawai NCTC 13529T (99.0â%), and less than 98.7â% similarity to other species of the genus Chryseobacterium with validly published names. The average nucleotide identity and in silico DNA-DNA hybridization values between F30T and the reference strains were 78.4-90.5â% and 2.6-42.5â%, respectively. The results of chemotaxonomic analysis indicated that the fatty acids, as well as the polar lipid profiles of F30T were similar to those of species of the genus Chryseobacterium, and the sole respiratory quinone was MK-6. On the basis of its phylogenetic, chemotaxonomic and phenotypic data, strain F30T represents a novel species, for which the name Chryseobacteriumaurantiacum sp. nov. is proposed. The type strain is F30T (=KCTC 62135T=MCCC 1K03457T).
Subject(s)
Chryseobacterium/classification , Phylogeny , Ponds/microbiology , Water Microbiology , Animals , Aquaculture , Bacterial Typing Techniques , Base Composition , China , Chryseobacterium/genetics , Chryseobacterium/isolation & purification , DNA, Bacterial/genetics , Fatty Acids/chemistry , Nucleic Acid Hybridization , Perciformes , Pigmentation , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Vitamin K 2/analogs & derivatives , Vitamin K 2/chemistryABSTRACT
A taxonomic study was carried out on strain LW15T, which was isolated from the external lesions of diseased farmed Murray cod (Maccullochella peelii peelii) from an intensive culture pond. Cells of strain LW15T were Gram-negative, facultative-anaerobic, non-motile, and both coccobacillus- and bacillus-shaped. Growth was observed at NaCl concentrations of 0-2â% (w/v) (optimum, 0â%), 4-32 °C (optimum, 25-28 °C) and pH 5.0-9.0 (optimum, 7.0). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain LW15T was affiliated to the genus Acinetobacter, showing the highest similarity to Acinetobacter guillouiae CIP 63.46T (97.7â%) and other Acinetobacter species with validly published names (93.5-97.6â%). Whole-genome sequencing and phylogeny reconstruction based on a core set of 1061 Acinetobacter genes indicated that strain LW15T was most closely related to the clade formed by A. guillouiae CIP 63.46T and Acinetobacter bereziniae CIP 70.12T and distantly related to any of the described species of genus Acinetobacter. Furthermore, strain LW15T could be distinguished from all known Acinetobacter species by its ability to assimilate ß-alanine and l-arginine, but not d-glucose. The principal fatty acids were C18â:â1ω9c, C16â:â0 and C16â:â1ω7c/C16â:â1ω6c. The major respiratory quinone was Q-9. Polar lipids of strain LW15T comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, four phospholipids, aminolipid and two unknown lipids. Based on its phenotypic and genotypic data, strain LW15T represents a novel species of the genus Acinetobacter, for which the name Acinetobacterpiscicola sp. nov. is proposed. The type strain is LW15T (=MCCC 1K03337T=CICC 24241T=KCTC 62134T=JCM 32101T).
