Identification and Cytotoxic Evaluation of Pregnane Saponins from the Twigs and Leaves of Dregea volubilis.

Four new polyhydroxy pregnane glycosides, named volubilosides G-K (3, 5-7), along with three known secondary metabolites, dregeoside Da1 (1), dregeoside Ka1 (2), and volubiloside E (4) were isolated from the twigs and leaves of Dregea volubilis (DV). The chemical structures of these compounds (1-7) were elucidated using spectroscopic techniques (1D and 2D NMR and HR-ESI-MS analyses) and compared with those in the published literature. Compounds (1-7) were evaluated for cytotoxicity against eight cancer cell lines (MB49, K562, MKN-7, HT29, A549, MCF-7, MDA-MB-231, and HepG2), revealing varying levels of cytotoxic effects with IC50 values ranging from 4.29 to 21.05 µM. The results indicated that compounds 1-7 may serve as potential lead compounds for the discovery and development of novel anti-cancer drugs.

The Preparation and Characterization of MnFe2O4-Decorated Expanded Graphite for Removal of Heavy Oils from Water.

Recently, many methods have been developed to efficiently eliminate oil spills due to its long-term harmful effects on marine life and human health. Expanded graphite (EG) has been considered as an excellent platform to remove contaminated oil from aqueous solution through a facile adsorption route. As an innovative approach, the decoration of magnetic components, namely, MnFe2O4, into graphite layers was taken into account for facilitating phase separation under magnetic field which resulted into an easy collection of the used adsorbents in a large scale. The expanded graphite/manganese ferrite composites were prepared from Vietnamese graphite flakes via a two-stage process. Characterization was performed using Scanning Electron Microscope (SEM), Fourier-Transform Infrared Spectroscopy (FTIR), X-Ray Powder Diffraction (XRD), Vibrating Sample Magnetometer (VSM), Energy-Dispersive X-ray (EDS), and nitrogen adsorption/desorption analysis. The adsorption behavior of EG-MnFe2O4 for widespread used heavy oils, including diesel oil and crude oil, was investigated under the effects of adsorption conditions, i.e., contact time, loaded oil dosage, and salinity of mixing oil and water. The obtained results showed successful incorporation of MnFe2O4 into graphite sheets and no considerable change on the worm-like structure of EG. The results also showed that incorporated manganese ferrites enhanced the magnetism EG up to 16 emu/g, which made the recovery of used adsorbent conveniently. The EG-MnFe2O4 adsorbents exhibited the strong adsorption ability toward diesel oil (32.20 ± 0.46 g DO/g EG) and crude oil (33.07 ± 0.33 g CO/g EG). In brief, EG-MnFe2O4 material provides a potential and promising platform with high performance for oil spill removal.

Dengue hemophagocytic syndrome: A systematic review and meta-analysis on epidemiology, clinical signs, outcomes, and risk factors.

We systematically searched and meta-analyzed the epidemiological characteristics, frequency of clinical signs, and outcomes of dengue-associated hemophagocytic lymphohistiocytosis. Ten electronic databases were searched systematically plus manual search of reference lists to identify relevant articles published until May 2017. The highest number of reported cases were from South-East Asia region (62 cases), followed by Western Pacific region (20 cases), and America (31 cases). The term "dengue hemorrhagic fever" predominated in studies that used the World Health Organization 1997 definition (59.7%), whereas "severe dengue" predominated in studies using the World Health Organization 2009 definition (76.8%). Among 122 cases, fever, splenomegaly, hepatomegaly, anemia, thrombocytopenia, and serum ferritin ≥500 µg/L were likely to report by articles representing by large sample size. The pooled proportion of these findings were as follows: fever 97.2%, hepatomegaly 70.2%, splenomegaly 78.4%, thrombocytopenia 90.1%, anemia 76.0%, and serum ferritin ≥500 µg/L 97.1%. This study highlighted a high case fatality rate (14.6%) and co-infection among dengue hemophagocytic lymphohistiocytosis patients. We suggest that long fever duration, persistent thrombocytopenia, elevated serum ferritin, and lactate dehydrogenase levels could be good diagnostic indicators for dengue-associated hemophagocytic syndrome. Bone marrow aspiration could be used as one criterion for diagnosis but is not obligatory. Further research is needed to examine the possible risk difference for development of hemophagocytic syndrome and to explore potential relationships between specific dengue classifications and dengue-associated hemophagocytic syndrome.