[Morphological manifestations of secretory activity of the neutrophils in inflammation]. / Morfologicheskie proiavleniia sekretornoi aktivnosti neitrofil'nykh leikotsitov pri vospalenii

Neutropholic leukocytes of aseptic peritoneal exudate of rabbits differed from neutrophilic leukocytes of the blood in greater variability in the content of cationic proteins. Some lysosomes ceased to be stained on cationic proteins, which produced a false impression of degranulation of leukocytes. Ultramicroscopically, there was observed a "clearing" of the lysosomes content and its outflow into cytoplasm. Lysosomes acquired the shape of spherical "holed" bodies and were transformed into clear vacuoles with remnants of the electron-dense material. The rupture of lysosome membranes led to an "explosive" type of liberation of the lysosome content into the cytoplasm. Partly degranulated leukocytes remained vital and activity moved about in a homologous serum. Following contacts with histone F1 leukocytes lost cationic proteins. The liberation of cationic proteins from leukocytic took place without destruction of leukocytes and lysosomes themselves.

[Morphological manifestations of the intracellular toxic effect of microorganisms of the ornithosis-lymphogranuloma-trachoma group]. / Morfologicheskie proiavleniia vnutrikletochnogo toksicheskogo deistviia u vozbuditelei iz gruppy ornitoza-limfogranulemy-trakhomy

Interactions of the causative agents of meningopneumonia and ovine abortion with pulmonary macrophages in experimental infection in white mice were studied by histological, immunofluorescent and electron microscopic methods. The causative agent of meningopneumonia did not multiply in colonies and caused toxic lesions in the cells and formed cytoplasmic oxiphilic inclusion bodies. Being the product of cell degeneration, these oxiphilic inclusion bodies were conducive to long-term survival of the cell and slow release from them of elementary bodies of meningopneumonia agent. The agent of ovine abortion multiplied in colonies without toxic affection of the cells or formation of oxiphilic inclusion bodies. It is suggested that oxiphilic inclusion bodies represent morphological manifestation of the intracellular toxic effect of rickettsial and ornithosis-like agents possessing dissociated (non-colonial) mode of multiplication.