Synergistic antihyperglycemic effects between plant-derived oleanolic acid and insulin in streptozotocin-induced diabetic rats.

Studies from our laboratories indicate that Syzygium cordatum leaf extract contains triterpene mixtures [oleanolic acid (OA) and ursolic acid (UA)] with hypoglycemic properties. The aims of this study were to investigate the hypoglycemic effects of Syzygium aromaticum-derived OA and whether OA influenced the blood glucose lowering effects of insulin in streptozotocin (STZ)-induced diabetic rats. We envisaged that OA may provide a strategy with different mechanism of action for effective diabetic therapy because no single-marketed antidiabetic drug is capable of achieving long-lasting blood glucose control. The effects of various doses of OA and/or standard antidiabetic drugs on blood glucose were monitored in nondiabetic and STZ-induced diabetic rats given a glucose load after an 18-h fast. Rats treated with deionized water and standard antidiabetic drugs acted as untreated and treated positive controls, respectively. Blood glucose concentrations were measured at 15-min intervals for the first hour and hourly thereafter for 3 h. Blood glucose concentrations were also monitored in animals treated with OA and/or standard antidiabetic drugs for 5 weeks. OA like insulin decreased blood glucose concentrations in nondiabetic and STZ-induced diabetic rats. Combined OA and insulin treatment had even greater antihyperglycemic response, suggestive of a synergistic effect of the two. After 5 weeks, STZ-induced diabetic rats exhibited hyperglycemia and depleted hepatic and muscle glycogen concentrations. OA treatment lowered the blood glucose with concomitant restoration of glycogen concentrations to near normalcy. Our results suggest that OA may have a role in improving insulin sensitivity. These findings merit further research in this field.

Renal effects of plant-derived oleanolic acid in streptozotocin-induced diabetic rats.

Previous studies from our laboratories indicate that the anti-diabetic effects of Syzygium cordatum (Hochst.) [Myrtaceae] leaf extract in streptozotocin-induced diabetic rats may be attributed in part to mixtures of triterpenes, oleanolic acid (3ss-hydroxy-olea-12-en-28-oic acid, OA) and ursolic acid (3ss -hydroxyl-urs-12-en-28-oic acid, UA). For the bioactive compounds to have potential in diabetes management, they should alleviate or prevent complications of diabetes mellitus, kidney function, and cardiovascular disorders. This study was, therefore, designed to assess whether S. cordatum leaf derived OA influenced renal function evaluated by the ability to increase urinary Na(+) outputs parameters and creatinine clearance (Ccr) of streptozotocin (STZ)-induced diabetic rats. Extraction and fractionation of S. cordatum powdered leaf ethyl acetate-solubles (EAS) yielded mixtures of OA/UA and methyl maslinate/methyl corosolate. Recrystallization of OA/UA mixture using ethanol afforded OA, the structure of which was confirmed by NMR spectroscopy ((1)H & (13)C). Acute effects of OA on kidney function and mean arterial blood pressure (MAP) were investigated in anesthetized rats challenged with hypotonic saline after a 3.5-h equilibration for 4h of 1 h control, 1.5 h treatment, and 1.5 h recovery periods. OA was added to the infusate during the treatment period. Chronic effects of OA were studied in individually caged rats treated twice daily with OA (60 mg/kg, p.o.) for five weeks. By comparison with respective control animals administration, OA significantly increased Na(+) excretion rates of non-diabetic and STZ-induced diabetic rats without affecting urine flow, K(+) and Cl(-) rates. At the end of five weeks, OA treatment significantly (p < 0.05) increased Ccr in non-diabetic (2.88 +/- 0.14 vs. 3.71 +/- 0.30 ml/min) and STZ-diabetic rats (1.81 +/- 0.32 vs. 3.07 +/- 0.16 ml/min) with concomitant reduction of plasma creatinine concentration (n = 6 in all groups). OA also caused significant decreases in MAP in non-diabetic and STZ-induced diabetic rats. These findings suggest that OA may have beneficial effects on some processes associated with renal derangement of STZ-induced diabetic rats.

Effects of Persea americana Mill (Lauraceae) ["Avocado"] ethanolic leaf extract on blood glucose and kidney function in streptozotocin-induced diabetic rats and on kidney cell lines of the proximal (LLCPK1) and distal tubules (MDBK).

Extracts of Persea americana Mill (Lauraceae) ("Avocado") have been traditionally used to treat hypertension and diabetes mellitus. Accordingly, we studied the hypoglycaemic and renal function effects of P. americana leaf ethanolic extracts (PAE) in STZ-induced diabetic rats. Oral glucose tolerance responses to various doses of PAE were monitored in fasted rats following a glucose load. Rats treated with deionized water or standard hypoglycaemic drugs acted as untreated and treated positive controls, respectively. Acute renal effects of PAE were investigated in anesthetized rats challenged with 0.077 M NaCl after a 3.5-h equilibration for 4 h comprising 1 h control, 1.5 h treatment and 1.5 h recovery periods. PAE was added to the infusate during the treatment period. Hepatic glycogen concentration was measured after 6 weeks of daily treatment with PAE. PAE induced dose-dependent hypoglycaemic responses in STZ-induced diabetic rats while subchronic PAE treatment additionally increased hepatic glycogen concentrations. Acute PAE infusion decreased urine flow and electrolyte excretion rates, whilst subchronic treatment reduced plasma creatinine and urea concentrations. These results indicate not only the basis of the ethnomedicinal use of P. americana leaf extract in diabetes management, but also of need for further studies to identify and evaluate the safety of PAE's bioactive compounds.

Optimal time of arrival for performance at moderate altitude (1700 m).

PURPOSE: The time course of physiological exercise responses after acute ascent to moderate altitude was investigated. METHODS: Fifteen young male subjects (16.5 +/- 0.8 yr) completed one familiarization and then two further sea level sessions to determine sea level values (SL). Subjects were then tested 6 h (ALT1), 18 h (ALT2), and 47 h (ALT3) after arrival at 1700 m. Subjects completed a 5-min submaximal cycle test, shuttle run test, and 45-s repeated push-up test. Hemoglobin (Hb) and hematocrit (Hct) were measured. RESULTS: Cycle test heart rate (HR) was higher at ALT1 than SL (182 +/- 15 vs 177 +/- 16 b.min(-1), P < 0.01) but had returned to 177 +/- 13 and 176 +/- 12 b.min(-1) at ALT2 and ALT3, respectively. At ALT1, only five subjects completed the full 5 min. Postexercise plasma lactate was not different. Shuttle test HR was higher at ALT1 than SL (191 +/- 8 vs 185 +/- 14 b.min(-1), P = 0.01) but had returned to 185 +/- 10 and 183 +/- 16 b.min(-1) at ALT2 and ALT3. Shuttle run time (SRT) was 37% shorter at ALT1 than SL (251 +/- 134 vs 401 +/- 115 s, P < 0.001) and remained impaired at ALT2 and ALT3 (330 +/- 124 and 344 +/- 115 s, both P < 0.001 vs SL). There was a significant relationship between magnitude of increased HR and reduction in SRT between SL and ALT1 (r = 0.52, P < 0.05). Push-up HR, Hb, and Hct were not affected by altitude. CONCLUSIONS: These findings indicate that detrimental effects of acute ascent to 1700 m on exercise performance are greater after 6 h than after 18 or 47 h. Performance was not entirely restored to SL values even after 47 h, despite restoration of easily measured physiological parameters. The results suggest travel to moderate altitude should occur as early as is practical before competition.

Towards reducing the trauma of direct intracompartmental pressure measurement for children: an in vitro assessment of small-diameter needles.

Direct intracompartmental pressure (ICP) measurement in children is an important investigation, the use of which may be inhibited by the invasive nature of the procedure and the large 18-gauge (G) needles that have hitherto been recommended. These large needles contribute to patient, parental, and physician anxiety. This study compares ICP measurements using needles of different lengths and port configuration, ranging from 18 to 25 G, against the "gold standard" 18 G, long, double-ported needle. All except the 18 G long, single-ported needle had limits of agreement within 3 mm Hg of the gold-standard needle. The results indicate that needle manometry can confidently be performed using small needles, and that the addition of a side-port is unnecessary in needles of small volume.

Nursing's self-image--nursing education's responsibility.

This article is concerned primarily with the development of a professional image of nurses and the significant role played by nursing educators. What do nursing educators do or should they do to enhance positive self-image among their students? After many years of dealing with students at various stages along the way toward RN, and seeing their reluctance to enter the nursing world due to uncertainty and fear, it seemed to the authors that the root of the problem may be in the development of professional self-image. The authors believe that even though curricula are sound and faculty well prepared for their responsibilities, students still develop these feelings of doubt and inadequacy. Perhaps nursing educators need to do a self-examination by answering the questions posed throughout the article. It is the belief of the authors that nursing educators sincerely subscribe to their role in self-image development. But, at the same time, they may unconsciously contribute to some aspects of negative image development. Is it something that they "do or don't do"? The article is intended to be thought provoking; to stimulate thinking and self-examination. It is not meant to introduce new and innovative material. It reiterates some facts, enumerates a variety of situations, provides examples, and poses many questions. It is divided into three areas of discussion. Self-concept is briefly reviewed as foundation material for the examples and situations enumerated. Further discussion centers on the importance of the individual (nurse) and the importance of the work (nursing).(ABSTRACT TRUNCATED AT 250 WORDS)