ABSTRACT
The comparative analysis of the immunohistochemical method and Ziehl-Neelsen staining was used to search for a new highly sensitivity procedure for diagnosing tuberculosis. With both methods, mycobacteria tuberculosis were detected in 10 cases of fibrocavernous pulmonary tuberculosis, 1 case of general tuberculosis, and 10 cases of disseminated lung lesion (sarcoidosis?). The improved immunohistochemical option using Mycobacterium tuberculosis antibodies (clone 1.1/3/1) have shown AEC and appeared to be the most sensitive than the Ziehl-Neelsen staining and may be therefore recommended for the diagnosis of tuberculosis.
Subject(s)
Antibodies, Bacterial/chemistry , Mycobacterium tuberculosis , Sarcoidosis, Pulmonary/diagnosis , Tuberculosis, Pulmonary/diagnosis , Humans , Immunohistochemistry , Mycobacterium tuberculosis/chemistry , Sarcoidosis, Pulmonary/microbiology , Sarcoidosis, Pulmonary/pathology , Sensitivity and Specificity , Tuberculosis, Pulmonary/microbiology , Tuberculosis, Pulmonary/pathologyABSTRACT
The number of nucleoli in lymphocyte nuclei was compared in the peripheral blood of the Chernobyl liquidators and non-irradiated persons (control). The former was significantly distinguished from the latter (p < 0.01) by the parameter "the number of nucleoli in lymphocytes", mean numbers of nucleoli per nucleus in these being 1.18 and 1.12, respectively. The increased number of nucleoli in lymphocytes of the Chernobyl liquidators may be associated with cytogenetic radiation effects.
