Subject(s)
Immunoglobulin G , Drug Stability , Glycerol , Mannitol , Protein Conformation , Sorbitol , Temperature , XylitolABSTRACT
In using a series of the succinic anhydride concentration the authors obtained preparations of succinylated albumin with a different degree of modification. The effect of succinylation on the immunochemical and some physico-chemical properties of albumin were investigated; depending on the concentration of the modifying reagent preparations of succinylated albumin proved to differ by the antigenic properties, electrophoretic mobility in agar gel and the complex-forming activity in interaction with rivanol. Conditions of partial modification permitting to increase the complex-forming properties of albumin 3-fold with the minimal changes in its structure were determined.
Subject(s)
Serum Albumin , Chemical Phenomena , Chemistry , Electrophoresis, Agar Gel , Epitopes , Humans , Protein Conformation , SuccinatesABSTRACT
Dynamics of specific binding of the antigen with rabbit blood lymphocytes in the process of immunization was studied by radioimmunochemical method with the use of human serum albumin, I125-labeled. Three types of response to the antigenic stimulant were revealed: biphasic with two maximal of the antigen-binding activity -- on the 5th--10th and the 15th--20th day; uniphasic -- with the maximum activity on the 15th--20th day, the absence of increase of the antigen-binding activity of blood lymphocytes and a high mean level of specific antigens; the absence of primary cell reaction to the administration of the antigen and low level of specific antibodies. A correlation was revealed between the intensity of the humoral response and the sum total amount of the immunocompetent lymphocytes entering the circulation in the course of the whole immunization period.
