Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Main subject
Language
Publication year range
1.
Rapid Commun Mass Spectrom ; 15(24): 2387-92, 2001.
Article in English | MEDLINE | ID: mdl-11746907

ABSTRACT

Individual peptides with lysine at the C-terminus as well as protein tryptic digests were reacted with 2-methoxy-4,5-dihydro-1H-imidazole, converting lysine residues to their 4,5-dihydro-1H-imidazol-2-yl derivatives. The mass spectra of derivatized digests exhibit a greater number of more intense features than their underivatized counterparts, thus increasing the information obtained in peptide mapping experiments. Additionally, MS/MS spectra of the derivatized peptides are greatly simplified in comparison to their native species, yielding primarily an easily interpretable series of y-ions. Finally, this novel label also enables differential quantitation studies, as a stable isotopic form containing four deuterium atoms can readily be produced.


Subject(s)
Proteins/chemistry , Amino Acid Sequence , Animals , Calibration , Horses , Hydrolysis , Imidazoles/chemistry , Indicators and Reagents , Lysine/analysis , Molecular Sequence Data , Myoglobin/analysis , Peptide Fragments/analysis , Proteome/analysis , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization , Spectroscopy, Fourier Transform Infrared , Trypsin
SELECTION OF CITATIONS
SEARCH DETAIL
...