ABSTRACT
III.-IV. stage, protein-calorie malnourished, pharyngolaryngeal tumorous patients were artificially fed perioperatively. Protein source (soya-protein concentrate, TOFU-soya-protein-cottage cheese) manufactured in Hungary, was administered in enteral feeding through naso-gastric tube. The metabolism, transport and utility of the soya-based diet were investigated by biochemical methods and compared to the previously administered tube-feeding. The method is advised on the base of the favourable experiences of the authors.
Subject(s)
Enteral Nutrition , Laryngeal Neoplasms/surgery , Pharyngeal Neoplasms/surgery , Adult , Dietary Proteins/administration & dosage , Female , Humans , Hungary , Laryngeal Neoplasms/complications , Laryngeal Neoplasms/physiopathology , Male , Middle Aged , Neoplasm Staging , Pharyngeal Neoplasms/complications , Pharyngeal Neoplasms/physiopathology , Protein-Energy Malnutrition/etiology , Protein-Energy Malnutrition/therapy , Soybean Oil/administration & dosageABSTRACT
The authors describe a modified version of counterimmunoelectrophoresis of easy performance and high sensitivity. With this method human haemoglobin can easily and specifically be determined either in fresh or dried biological samples. Glutaraldehyde pretreatment of the samples results in the development of complexes with highly favourable electrophoretic mobility and precipitating capacity. By this chemical modification and use of a double set of samples with varying antigen-antibody proportion, the sensitivity of haemoglobin detection in erythrocyte-containing haemolysate and native blood was 300 ng/ml. The examination is easily performed, and without considerable outlay, even with conventional laboratory facilities. It is equally suitable for the detection of occult colorectal bleeding and for species-specific study of blood stains of unknown origin. The authors succeeded in identifying human haemoglobin even in a 42-month dried blood stain.
Subject(s)
Counterimmunoelectrophoresis/methods , Hemoglobins/analysis , Blood Stains , Colorectal Neoplasms/diagnosis , Counterimmunoelectrophoresis/statistics & numerical data , Evaluation Studies as Topic , Glutaral , Hemoglobins/immunology , Humans , Immunochemistry , Occult Blood , Sensitivity and Specificity , Species SpecificityABSTRACT
Besides the classical lesions, symmetric necrosis was found in the thalamus of pyrithiamine treated rats. The barrier systems of the two areas differ already in healthy animals and behave differently during the illness.
