ABSTRACT
This article reports a case of Kawasaki disease (KD) and its rapid fatal course in a 5-year-old boy, who 3 days before hospitalization demonstrated fever and diffuse erythema in the face, neck, and torso, as well as swelling and pain below the right earlobe. During the admission, he was diagnosed with mumps and suspected scarlet fever. Abnormal laboratory findings included elevated values of procalcitonin, C-reactive protein, and interleukin 6. Sudden death occurred 8 days after admission. Autopsy confirmed the cause of death to be pericardial tamponade due to a ruptured, inflamed aneurysm of the left anterior descending coronary artery. We believe that any typical clinical sign of KD whenever associated with elevated indices of inflammation should set off suspicion of KD and further permit cardiovascular examination. This would contribute to distinguishing KD from other diseases with similar clinical signs in order to accelerate appropriate treatment.
Subject(s)
Aneurysm, Ruptured/pathology , Coronary Aneurysm/pathology , Death, Sudden/etiology , Mucocutaneous Lymph Node Syndrome/diagnosis , Cardiac Tamponade/etiology , Child, Preschool , Coronary Vessels/pathology , Humans , MaleABSTRACT
AIMS: Early diagnosis is important for cervical cancer treatment. This study aimed to characteriz the microRNA profile and target gene protein levels of cervical cancers in Uygur women for application in early diagnosis. METHODS: The profiles of miRNA in cervical cancer and chronic cervicitis were analyzed with miRNAmicroarray V4.0. The expression of miR-101 was detected by real-time PCR and locked nucleotide acid in situ hybridization (LNA-ISH). Cox-2 protein levels were assessed by immunohistochemistry. RESULTS: The microarray identified a set of 12 miRNAs significantly decreased in cervical cancer in comparison to the control group. Quantitative RT-PCR analysis showed miR-101 to be significantly downregulated in cancer tissues (p<0.05) while LNA-ISH showed miR-101 positive rates of 80% (20/25) and 8% (5/25) (p<0.05) in the control and cervical cancer groups. Cox-2 positive rates of cervical cancer and control groups were 84% (21/25) and 8% (2/25) (p<0.05). CONCLUSIONS: Use of down-regulation of miR-101 and up-regulation of Cox-2 as markers may play a role in early diagnosis of cervical cancer in Uygur women.
Subject(s)
Biomarkers, Tumor/genetics , Carcinoma/genetics , Cyclooxygenase 2/analysis , MicroRNAs/genetics , Uterine Cervical Neoplasms/genetics , Biomarkers, Tumor/analysis , Carcinoma/chemistry , Carcinoma/diagnosis , Case-Control Studies , China , Early Detection of Cancer , Female , Humans , Transcriptome , Uterine Cervical Neoplasms/chemistry , Uterine Cervical Neoplasms/diagnosisABSTRACT
Overexpression of several aquaporins (AQPS) has been reported in different types of human cancer but roles in human carcinogenesis have yet to be clearly defined. Here, we up-regulated expression of the AQP8 gene in SiHa human cervical cancer cells with a lentivirus transfection system and investigated its effects as a potential therapeutic target for cervical cancer. Results showed AQP8 overexpression did not affect their substrate adherence and proliferation, but accelerated migration as assessed by transwell migration and wound healing assays. Moreover, AQP8 overexpression significantly enhanced local invasion of SiHa cells in nude mice. These findings altogether indicate that AQP8 overexpression increases migration of SiHa cells and probably participates in the process of tumor local invasion.
