Subject(s)
Gene Products, gag/metabolism , Retroelements/physiology , Retroviridae/metabolism , Cell Membrane/metabolism , Escherichia coli/genetics , Gene Products, gag/biosynthesis , Gene Products, gag/genetics , Hydrogen-Ion Concentration , Ions/metabolism , Macromolecular Substances , Phosphorylation , RNA/metabolism , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Retroviridae/genetics , Retroviridae/physiology , Virus Assembly/physiologySubject(s)
Escherichia coli/genetics , Retroelements , Transcription Factors/genetics , Amino Acid Sequence , Bacteriophage T7/genetics , Base Sequence , Cloning, Molecular , DNA, Recombinant , Electrophoresis, Polyacrylamide Gel , Molecular Sequence Data , Open Reading Frames , Recombinant Proteins/geneticsABSTRACT
It is demonstrated by filter-binding assay that casein kinase 2 from Rana temporaria oocytes binds rRNA in vitro with high affinity. Ligand-blotting shows that rRNA-binding activity is inherent to alpha and alpha' subunits of the enzyme. Increase of pH from 6.5 to 7.5 has little effect on casein kinase but completely suppresses rRNA-binding activity of the enzyme. Sedimentation coefficient of casein kinase 2 also depends on pH: at pH 7.5 it is mainly 10 S, and at pH 6.5-18 S. At pH 6.95 the amounts of both forms are equal. The heavy form of casein kinase 2 practically lacks rRNA-binding activity.
Subject(s)
Oocytes/enzymology , Protein Kinases/metabolism , RNA, Ribosomal/metabolism , Animals , Casein Kinases , Female , Hydrogen-Ion Concentration , Kinetics , Macromolecular Substances , Molecular Weight , Protein Binding , Protein Kinases/isolation & purification , Rana temporariaABSTRACT
The variability of Q-heterochromatin regions (Q-HR) was studied in native residents of the northern part of West Siberia, viz Yakuts (n = 127), Selkups (n = 90) and Khants (n = 54), as well as in newcomers including oil-borers (n = 43) and children (n = 113) living permanently in this part of the USSR. The major quantitative characteristics of chromosomal Q-HR variability were shown to be very similar in oil-borers and natives, and this is considered to be the result of specific selection of individuals according to the amount of Q-HRs in their genome. The hypothesis on the possible selective value of chromosomal Q-HRs in human adaptation to extreme environmental conditions of the extreme north is discussed.
