Do PON1-Q192R and PON1-L55M polymorphisms modify the effects of hypoxic training on paraoxonase and arylesterase activity?

BACKGROUND: Low levels of antioxidant paraoxonase 1 (PON1) enzyme activity, PON1-Q192R polymorphism (a glutamine (Q) to arginine (R) substitution at position 192), PON1-L55M polymorphism (a leucine (L) to methionine (M) substitution at position 55), and oxidized low-density lipoprotein (oxLDL) are risk factors for coronary heart disease. Aerobic exercise improves PON1 activity, but the effects of hypoxic exercise are yet unclear. The aim of this study was to determine the effects of hypoxic underwater rugby training on PON1 activity and oxLDL levels and the role of the mentioned polymorphisms. METHODS: Serum PON1 and arylesterase activities (ARE), PON1, PON3, and oxLDL protein levels (by using the enzyme-linked immunosorbent assays) were determined in an athletic group (42 trained male underwater rugby players; age = 21.7 ± 4.2 years, mean ± SD) and a control group (43 sedentary men; age = 23.9 ± 3.2 years). The polymorphisms were determined from genomic DNA samples. RESULTS: PON1 activity (25.1%, p = 0.052), PON3 (p < 0.001), and oxLDL (p < 0.001) of the athletic group, including most genotype groups, were higher than those of the control group. In comparison to the controls, PON1 activity levels (p = 0.005) of the PON1-Q192R homozygote QQ genotype group and PON1 activity levels (30%, p = 0.116) of the PON1-L55M homozygote LL genotype group were higher, whereas ARE activity values of athletic R allele carrier (Rc = QR + RR) (p = 0.005) and LL group (p = 0.002) were lower than the control genotype groups related to their polymorphisms. CONCLUSION: Hypoxic training can cause (1) significant oxidative stress, including oxLDL, and an antioxidant response (increase in PON1 activity and PON3), (2) differences in the activity of PON1 and ARE, which are modified by PON1-Q192R and PON1-L55M polymorphisms, respectively, and (3) improvements in PON1 activity of QQ and LL groups. However, hypoxic training can cause a disadvantage of LL and Rc groups for ARE.

Does the TMPRSS6 C > T Polymorphism Modify the Endurance Training Effects on Hematological Parameters?

This study investigated the role of TMPRSS6 C > T polymorphism (TMPRP) on the effects of chronic aerobic training on main hematological parameters in male soccer referees, which is yet unknown. Two groups composed of total of 45 healthy male soccer referees and 42 sedentary were compared for hemogram, serum hepcidin, ferritin, and iron levels. TMPRP was determined from genomic DNA samples. Participants' physical and physiological (Yoyo endurance level-2 test) measurements were carried out. The athletic T carrier (Tc = TT + TC) group RBC count was significantly higher than the control (p < 0.01), whereas the athletic CC homozygous group serum iron and transferrin saturation (TS) were lower than the control depending on the TMPRP. The ferritin and iron values of the athletic Tc group were higher than of the athletic CC group (29.2% and 14.1%, respectively; p > 0.05) although the control Tc group RBC (p < 0.05) and iron (23.8%, p > 0.05) values were lower than the control CC due to genetic tendency. The training did not change hepcidin levels. These results suggest that the TMPRP can modify the endurance training effects on iron and TS levels and RBC count (in the CC and Tc groups) respectively. The CC group may be adversely affected for iron and TS from endurance trainings. It may be recommended that the training programs should be organized according to phenotype characteristics.

The effects of regular aerobic exercise training on blood nitric oxide levels and oxidized LDL and the role of eNOS intron 4a/b polymorphism.

BACKGROUND: Nitric oxide (NO), oxidized LDL (OxLDL) and endothelial nitric oxide synthase intron 4a/b polymorphism (eNOSP) are related to atherosclerosis (AS). The present study investigated the effects of regular aerobic exercise training on the mentioned risk factors as well as blood lipids and lipoproteins (BLLPs) and the role of eNOSP, which is unclear. METHODS: The study was participated by 46 well trained male soccer referees as the athletic group (AG, age; 23.26 ± 2.84 years) and 43 sedentary controls (CG, age; 23.16 ± 3.28 years). Yoyo intermittent endurance (Yoyo IE-2 test) was performed to measure aerobic endurance levels of the participants. Serum NO, eNOS and oxidized LDL (OxLDL) levels (by ELISA method) and total oxidant /antioxidant status ratio (/TOS/TAS) as oxidative stress (OS) index (OSI) and BLLPs levels were determined. eNOSP was identified from genomic DNA samples with VNTR analysis. RESULTS: There is no significant difference between AG and CG including the genotype groups for NO, eNOS and BLLPs and eNOSP has no role. However, AG's NO (29%, p > .05) and TAS levels were significantly higher (p = .001) than those of CG, whereas OSI (p = .001) and OxLDL (p = .011) values were significantly lower. On the other hand, NO value of the athletic bb group was 29% higher compared with the control and the a carrier (aC = aa + ab) group. CONCLUSIONS: These findings suggest that regular aerobic exercise improves blood NO levels and antioxidant capacity, while decreasing OS levels including OxLDL, but not eNOS and BLLPs in the athletes. Although the polymorphism does not have a modifying effect on these effects, bb genotype group may benefit more from training for NO than aC group due to genetic tendency.

Effects of aerobic training on serum paraoxonase activity and its relationship with PON1-192 phenotypes in women.

BACKGROUND: Paraoxonase 1 (PON1) is an antioxidant enzyme that protects high-density lipoprotein (HDL) and low-density lipoprotein against oxidation. Limited studies have addressed the influence of exercise on PON1 activity and its relationship with PON1 phenotypes. We investigated relationships between PON1-192 phenotypes, PON1 activity, aerobic exercise, and blood lipid and lipoprotein concentrations in middle-aged women. METHODS: An exercise group (n = 50) engaging in regular aerobic exercise and a control group (n = 41) were selected from a subset of 300 Caucasian women that met the inclusion criteria. Serum PON1, salt-stimulated PON1 (SSPON1), and arylesterase (ARE) activities; cholesterol levels and ARE activities of total HDL and HDL subgroups (HDLs) (supernatants obtained by polyethylene glycol); and blood lipid and lipoprotein concentrations were determined by standardized enzymatic methods. PON1-192 QQ (low activity), QR (moderate activity), and RR (high activity) phenotype groups were defined using serum SSPON1/ARE activity ratios. The R-carries (RC) phenotype group consisted of the QR and RR groups combined. RESULTS: All lipid and lipoprotein concentrations were greater in the exercise group than in the control group. Regardless of phenotype, no significant differences were observed between the exercise and control groups in terms of serum PON1, SSPON1, or ARE activity associated with HDLs (p > 0.05), whereas PON1 activities in QQ-phenotyped women in the exercise group were significantly higher than those in the control group (p < 0.01), but not the RC group. A statistically significant interaction between PON1 phenotypes (QQ and RC groups) and exercise (exercise and control groups) on PON1 activity was found. CONCLUSION: These results showed that a regular aerobic exercise program can improve PON1 activity depending on PON1-192 phenotype, but not on lipid and lipoprotein levels, in middle-aged Turkish women.

Effects of anaerobic training on paraoxonase-1 enzyme (PON1) activities of high density lipoprotein subgroups and its relationship with PON1-Q192R phenotype.

AIM: Paraoxonase-1 (PON1) is an antiatherosclerotic enzyme located on high-density lipoprotein (HDL). The effects of anaerobic exercise on PON1 activity are unknown. Here we investigated the effects of anaerobic judo training on three different activities of same PON1 enzyme (TDPON1), including basal PON1, salt-stimulated PON1 (SPON1), and arylesterase (AE) activities, of serum, HDL, and HDL subgroups (HDLs; HDL and its subgroups) and its relationship with PON1-Q192R phenotype (PON1P). METHODS: Our study included 18 Turkish national female judoists (mean age: 17.9 ± 0.8 years). Before and after 5 months of anaerobic training, critical speed (CS), TDPON1 activities, cholesterol levels in the serum and supernatants of HDLs obtained by polyethylene glycol, and other major blood lipids and lipoproteins (BLLPs) including triglycerides were determined using blood samples taken after overnight fasting. PON1P groups (PGs) were categorized as QQ (QG; persons with low activity) and R carriers (QR + RR) (RG; persons with high activity) according to SPON1/AE activity ratios. The results were considered statistically significant at P ≤ 0.05. RESULTS: Anaerobic training resulted in significantly increased the cholesterol levels of HDLs (except HDL2-C) in all subjects, but not HDLs-C in PGs. Anaerobic training resulted in significant increases in most TDPON1 activities of serum and HDLs in all subjects and (except AE) in PGs, whereas SPON1 and HDL2 AE activities increased only in the RG, which was related to PON1P. However, PON1P was not related to other measured markers, including basal BLLP profiles. CONCLUSIONS: Anaerobic training improved most TDPON1 activities of serum and HDLs and HDLs -C levels (except HDL2-C) in all subjects, but not HDLs-C in PGs. The beneficial effects of anaerobic training on SPON1 and HDL2 AE activities were depend on PON1P. The lack of response of HDL2-C to anaerobic exercise will require further research.

The relationships between simulated tennis performance and biomarkers for nitric oxide synthesis.

Tennis performance requires a good aerobic endurance and recovering capacity. Nitric oxide (NO) is a gas which is not only a vasodilator and antioxidant but it also regulates the use of oxygen and glucose. The aim of this study was to examine the relationships between simulated tennis performance test (PT) and NOx (sum of nitrate+nitrite) levels and lactate elimination speed (LES). Twenty well trained male tennis players with game levels of ITN 4 (International Tennis Number) and lower (mean ± SD; age 22.9 ± 2.6 yrs; height 1.82 ± 0.06 m and mass 75.7 ± 8.0 kg) participated in the study. Participants performed three 4-min bouts and a 2-min continuous groundstroke against balls projected from a tennis ball machine at speeds of 50, 55, 62 and 70 km·h(-1). After this exercise, subjects were given a 20 min passive rest. After each period and at during the recovery phase; plasma NOx, glucose (GLU) and lactate (LA) levels were determined. LES was calculated during passive recovery. GLU, LA and heart rate (HR) showed a linear increase in comparison to the values in the previous step while PT decreased significantly. Following each period NOx and glucose levels increased independently, but their decreasing rates in recovery phase were related (r = 0.470, p < 0.05). The successive increase in NOx and GLU parameters between the third and the forth periods was significant (p < 0.05). Only in the third period was there a significant relation between PT and NOx (r = 0.494; p < 0.05). In the present study, no significant relationship was found between PT and GLU, LA levels and LES. No significant correlation was found between simulated tennis performance and blood NOx levels. However the addition of loads like those in the third period in tennis trainings can be beneficial for performance in trained tennis players. It is recommended that the relationships between tennis performance with NOx and GLU are studied during a real tennis match. Key PointsIn a sport like tennis which lasts 2-6 hours and has short rests, performance depends on the player's capacity to perform the intensive exercise intermittently. Therefore, recovering potential is important in tennis.In none of the periods of this study were the expected (significant) relations observed of the athletes. Therefore, it can be claimed that LA increase, LA elimination and aerobic endurance do not play a significant role in the performance and the decrease in performance in this exercise model.