Evaluation of environmental effects of heavy metals on biochemical profile and oxidative stress among children at brick kiln sites.

The present study was designed to study the health risks among children living at brick kiln industries. A survey was conducted, questionnaires were filled out, and demographic data was collected from Punjab, Pakistan. Heavy metals burden and BMI were calculated, hematological and enzyme analysis, comet assay and hormonal ELISA were performed. The results showed decreased BMI, RBC count and hematocrit in the exposed group. Nickel, cadmium, zinc and chromium concentrations in whole blood were detected among exposed children. Antioxidant enzymes and growth hormone concentration decreased, while reactive oxygen species and cortisol level increased in the exposed group. The comet assay findings showed decreased percentage DNA in the head and increased in the tail region among exposed group. It was concluded that children living at brick kiln sites experienced decreased BMI, altered antioxidant enzymes status and hormone levels and cellular DNA damage that pose a major threat on child health.

Study of occupational exposure to brick kiln emissions on heavy metal burden, biochemical profile, cortisol level and reproductive health risks among female workers at Rawat, Pakistan.

The present study was planned to understand the heavy metal burden and its possible actions in blood of occupational females working at brick kilns at Rawat, Pakistan. A total of 232 women were included in the study, of which 114 presented control subjects. Apart from collection of demographic data, fertility indicators and body mass index (BMI), blood was collected from subjects that was later used for the determination of heavy metal concentrations using atomic absorption spectroscopy and haematological profile. Blood was centrifuged and plasma was obtained and stored at - 20° to study biochemical variables (sodium dismutase, peroxidase, reactive oxygen species, thiobarbituric acid reactive species, protein estimation), lipid profile and cortisol concentrations among the two groups. Analysis of heavy metal in blood showed elevated levels of cadmium (3.09 ± 0.01 µg/dl), chromium (4.20 ± 0.02 µg/dl) and nickel (5.59 ± 0.03 µg/dl) in worker's group as compared with control. Increased platelet count; decreased antioxidant enzyme and increased oxidants level; increased total cholesterol, low-density lipoprotein (LDL) and triglyceride (TG); decreased total protein and high-density lipoprotein (HDL); and increased cortisol levels were evident among workers as compared with the control group. The study concluded that occupational workers experience increased heavy metals burden in blood and, therefore, pose a risk to human health by causing reduction in antioxidant enzymes concentration and increase in stress conditions.

Impact of low-dose chronic exposure to bisphenol A and its analogue bisphenol B, bisphenol F and bisphenol S on hypothalamo-pituitary-testicular activities in adult rats: A focus on the possible hormonal mode of action.

Bisphenol A an estrogen-mimic endocrine disrupting chemical, used to manufacture polycarbonate plastics and epoxy resins with toxic effects for male reproduction. Due to its toxicity, industries have started to replace it with other bisphenols. In this study, the toxicity of BPA analogues (BPB, BPF and BPS) was evaluated in a chronic study. We investigated whether the chronic exposure to low bisphenols doses affects spermatogenesis with outcomes on oxidative stress and male reproductive system. Male rats (22 day old) were exposed to water containing 0.1% ethanol for control or different concentrations of BPA and its analogues BPB, BPF and BPS (5, 25 and 50 µg/L) in drinking water for 48 weeks. Results of the present study showed a significant alteration in the gonadosomatic index (GSI) and relative reproductive organs weights. Oxidative stress in the testis was significantly elevated while sperm motility, Daily sperm production (DSP) and number of sperm in epididymis were reduced. Plasma testosterone, LH and FSH concentrations were reduced and estradiol levels were high in 50 µg/L exposed group. These results suggest that exposure to BPA and its analogues for chronic duration can induce structural changes in testicular tissue and endocrine alterations in the male reproductive system.