[Features of metabolism of Klebsiella genus bacteria]. / Osobennosti metabolizma bakterii roda Klebsiella.

Some metabolic peculiarities of bacteria of the genus Klebsiella were investigated. The bacteria under study were isolated from different sources and varied in virulence. The pathogenic and saprotrophic enterobacteria were discriminated based on their response to the addition of carbohydrates or nitrate to the medium. Pathogenic Klebsiella spp. exhibited mainly the mixed formic-acid type of fermentation and were more resistant to nitrates than saprotrophic bacteria with the butanediol type of fermentation. The bacteria Klebsiella pneumoniae subsp. pneumoniae isolated from different sources, such as patients, healthy persons, or the environment, exhibited no substantial differences in metabolism and virulence. It was inferred that these bacteria themselves cannot cause disease, and their isolation in morbid states is an indirect result of dysbacteriosis.

[The content of mimicry antigens in bacteria of the genus Klebsiella when they are cultured in plant tissue]. / Soderzhanie antigenov mimikrii u bakterii roda Klebsiella pri kul'tivirovanii ikh v rastitel'noi tkani.

Bacteria of genus Klebsiella were found to possess group-specific antigens of ABO system. Representatives of conditionally pathogenic species, K. rhinoscleromatis, K. pneumoniae, K. ozaenae, K. oxytoca, contain 9.4 to 38% of the antigens. In the process of interaction of plant organisms and Klebsiella, which passed three times in green tomato fruits, the latter have been found to influence some biological features of the bacteria which is manifested in the loss of the mimicry antigens by a considerable part of the strains as well as in developing capsules by some capsule-free variants of klebsiellae.

[The antagonistic activity of spore bacteria in relation to representatives of the genus Erwinia]. / Antagonisticheskaia aktivnost' sporovykh bakterii po otnosheniiu k nekotorym predstaviteliam roda Erwinia.

Strains of 26 species of genus Bacillus were examined for an antagonistic effect relative to erwiniosis agents: Erwinia amylovora, Erwinia carotovora, Erwinia chryzanthemi, Erwinia herbicola, Erwinia rhapontici. The Erwinia strain growth seized in the process of combined cultivation with microbes-antagonists. Practically all the studied strains of bacteria of genus Erwinia were sensitive to these or those Bacillus cultures. A considerable number of Erwinia strains studied were sensitive simultaneously to several cultures of Bacillus subtilis. The mechanism of action of antagonistic substances excreted by a definite strain of B. subtilis and damaging cells of bacteria of the Erwinia genus is studied. A damage of K+ ions' transport via cell walls of sensitive phytopathogenic bacteria after treatment of their cells with filtrates of culture liquid of active B. subtilis strain is found.

[The antimicrobial spectrum of the action of bacteriocins and bacteriophages from Klebsiella strains]. / Issledovanie antimikrobnogo spektra deistviia bakteriotsinov i bakteriofagov nekotorykh shtammov Klebsiella.

The percentage of bacteriocin-producing and phage-producing Klebsiella strains was as follows: K. pneumoniae-10%, K. ozaenae-7%, K. rhinoscleromatis-9%. The antimicrobial spectrum of the studied inducible particler was broad and was not limited by the frames of the genus and family. Bacteriocins and bacteriophages from Klebsiella were active to Klebsiella, Enterobacter, Escherichia, Shigella and Proteus representatives significant in medicine. Klebocins and Klebsiella phages exhibited antagonistic effects to phytopathogenic bacteria. Some strains of Erwinia and Pseudomonas were sensitive to phages or bacteriocins from Klebsiella. Bacteriocins protected corn and tomato seeds from contamination by erwinioses agents. All cultures of Agrobacterium, Corynebacterium, Micrococcus, Staphylococcus, Streptococcus were resistant to action of phages and klebocins. Bacteriocins from Klebsiella were assayed for their sensitivity to trypsin, chymotrypsin, lysozyme, ribonuclease, deoxyribonuclease. Action of klebocins was associated with a protein component. Proceeding from data of diffusion through the disc ultrafiltration membranes molecular weight of klebocins was in the range of 30,000 and 50,000 Da.

[The physiological-biochemical properties of Klebsiella pneumoniae strains of different origins]. / Fiziologo-biokhimicheskie svoistva shtammov Klebsiella pneumoniae razlichnogo proiskhozhdeniia.

While comparatively analyzing the properties of Klebsiella pneumoniae from three studied sources (patients, healthy people, environment), no reliable differences between the comparable strains have been revealed. No differences have been found in the toxin formation, hemagglutinating and antilysozymic activity, adhesivity and invasive properties as well as in the peculiarities of gas exchange, while growing on media with different nitrogen and carbon sources. The studied microorganisms differed only in antibiotic resistance: strains isolated from the environment were sensitive to antibiotics, whereas strains of human origin were resistant to them.

[The antilysozyme activity of bacteria in the genus Klebsiella]. / Izuchenie antilizotsimnoi aktivnosti bakterii roda Klebsiella.

The ability to inactivate lysozyme was found in representatives of three species of the genus Klebsiella bacteria: K. pneumoniae (117 strains), K. rhinoscleromatis (104 strains), K. ozaenae (90 cultures). The test cultures displayed a different antilysozyme activity, inactivating from 2 to 30 micrograms/ml of the enzyme. Taking into account the lysozyme role in the immunity and chitin synthesis processes in the organism of insects, the inactivation of the enzyme by Klebsiella may be considered as one of possible mechanisms of the entomopathogenic action of these bacteria.

[Change in the state of the nuclear structures of neutrophilic leukocytes in the process of their specific alteration]. / Izmenenie sostoianiia iadernykh struktur neitrofil'nykh leikotsitov v protsesse ikh spetsificheskoi al'teratsii.

Investigation of the character of the luminiscent spectrum of nuclear structures in periferal blood leucocytes stained with acridine orange showed that a specific alteration of leucocytes of an animal sensitized by Klebsiella rhinoscleromatis causes an increase in the values of the luminescence relative intensity Jrel=J640 divided by J530. This indicates to DNA denaturation, occuring in the process of alteration.

[Characteristics of the interaction of Klebsiella rhinoscleromatis DNA with the sera from patient with systemic lupus erythematos]. / Osobennosti vzaimodeistviia DNK Klebsiella rhinoscleromatis s syvorotkami bol'nykh sistemnoi krasnoi volchankoi

In blood sera of patients with systemic red lupus there were observed antibodies, which interacted in reaction of indirect hemagglutination and in complement fixation test with DNA of scleromic bacteria and did not react with the other structures of bacterial cell: with Buaven antigen, detergent and capsular polysaccharides. Reaction of indirect hemagglutination of scleromic DNA with the sera was inhibited by DNA preparations of animal origin. The serological activity of DNA from scleromic bacteria depended upon its secondary structure and did not correlate with polymeric state and nucleotide composition of the biopolymer.