ABSTRACT
The integration of Au and Ag into nanoalloys has emerged as an intriguing strategy to further tailor and boost the plasmonic properties of optical substrates. Conventional approaches for fabricating these materials via chemical reductions of metal salts in solution suffer from some limitations, such as the possibility of retaining the original morphology of the monometallic substrate. Spontaneous nanowelding at room temperature has emerged as an alternative route to tailor Au/Ag nanomaterials. Herein, we perform a thorough study on the cold-welding process of silver nanoparticles onto gold substrates to gain a better understanding of the role of different variables in enabling the formation of well-defined bimetallic structures that retain the original gold substrate morphology. To this end, we systematically varied the size of silver nanoparticles, dimensions and geometries of gold substrates, solvent polarity and structural nature of the polymeric coating. A wide range of optical and microscopy techniques have been used to provide a complementary and detailed description of the nanowelding process. We believe this extensive study will provide valuable insights into the optimal design and engineering of bimetallic plasmonic Ag/Au structures for application in nanodevices.
ABSTRACT
Low molecular weight thiols (biothiols) are highly active compounds extensively involved in human physiology. Their abnormal levels have been associated with multiple diseases. In recent years, major efforts have been devoted to developing new nanosensing methods for the low cost and fast quantification of this class of analytes in minimally pre-treated samples. Herein, we present a novel strategy for engineering a highly efficient surface-enhanced Raman scattering (SERS) spectroscopy platform for the dynamic sensing of biothiols. Colloidally stable silver nanoparticles clusters equipped with a specifically designed azobenzene derivative (AzoProbe) were generated as highly SERS active substrates. In the presence of small biothiols (e.g., glutathione, GSH), breakage of the AzoProbe diazo bond causes drastic spectral changes that can be quantitatively correlated with the biothiol content with a limit of detection of ca. 5 nM for GSH. An identical response was observed for other low molecular weight thiols, while larger macromolecules with free thiol groups (e.g., bovine serum albumin) do not produce distinguishable spectral alterations. This indicates the suitability of the SERS sensing platform for the selective quantification of small biothiols.
Subject(s)
Metal Nanoparticles , Spectrum Analysis, Raman , Azo Compounds , Glutathione , Humans , Metal Nanoparticles/chemistry , Silver/chemistry , Spectrum Analysis, Raman/methods , Sulfhydryl CompoundsABSTRACT
Surface-enhanced Raman (SERS) spectroscopy has been establishing itself as an ultrasensitive analytical technique with a cross-disciplinary range of applications, which scientific growth is triggered by the continuous improvement in the design of advanced plasmonic materials with enhanced multifunctional abilities and tailorable surface chemistry. In this regard, conventional synthetic procedures yield negatively-charged plasmonic materials which can hamper the adhesion of negatively-charged species. To tackle this issue, metallic surfaces have been modified via diverse procedures with a broad array of surface ligands to impart positive charges. Cationic amines have been preferred because of their ability to retain a positive zeta potential even at alkaline pH as well as due to their wide accessibility in terms of structural features and cost. In this review, we will describe and discuss the different approaches for generating positively-charged plasmonic platforms and their applications in SERS sensing.
ABSTRACT
Herein we illustrate an effective protocol to boost the optical enhancing properties of gold nanostars. By coating single nanostars with a mesoporous silica layer of the appropriate size (yolk capsules), to localize them under optical microscopy, it is possible to enumerate single particles and design SERS quantitative methods with minute amounts of metallic particles.
