Parental Psychological Control and Self-Efficacy as Predictors of Romantic Relationship Power Dynamics.

In the current study, we utilized a person-centered approach to examine the relations of parental psychological control (PPC) and relationship self-efficacy (RSE) to power dynamics in emerging adults' romantic relationships. College student emerging adults (N = 312) completed measures assessing retrospective PPC, RSE, and perceived self and partner power in current relationships. Latent profile analysis uncovered four relationship types based on reported self- and partner-power: balanced-unified, balanced-interchanging, unbalanced-high self, and unbalanced-high partner. Increases in PPC were related to increased odds of being in an unbalanced relationship. Higher levels of RSE were associated with decreased odds of being in an unbalanced relationship. Further, even individuals in the balanced profile who reported average levels of both self and partner power (balanced-interchanging) reported higher levels of PPC and lower levels of RSE compared to those in the balanced group where levels of both self and partner power were low (balanced-unified). These findings suggest using a person-centered approach to relationship power may advance our conceptualization of power distribution in romantic relationships. Further, experienced family dynamics and one's sense of self may be especially important for young adults' tendency to form healthy relationships. The current findings encourage future investigation into the mechanisms by which parental factors predict both dominance and submissiveness in romantic relationships. Understanding predictors of power dynamics may contribute to intimate partner violence prevention and intervention.

Help From a Friend: Helicopter Parenting Predicts Empathy and Bystander Intervention.

Harassment toward others happens in many contexts with a myriad of negative impacts on victims, witnesses, and society. Although preventing harassment of others is ideal, it is also important to consider how bystanders may react in ways to defend the victim and reduce the harassment. Bystanders differ in their reactions to these events and the goal of this investigation is to better understand individual differences in college students' reported tendency to defend victims of harassment. We proposed a mediation model where higher rates of helicopter parenting would predict lower empathic concern and greater personal distress. In turn, lower empathic concern and greater personal distress would predict lower likelihood of defending the victim. College students (n = 305) completed self-report measures of helicopter parenting, empathic concern, personal distress, and bystander intervention to general harassment. Using the Hayes PROCESS program, we found the relation of helicopter parenting to bystander intervention was mediated by empathic concern, such that helicopter parenting predicted lower empathic concern, which predicted lower likelihood of intervening. Helicopter parenting predicted greater personal distress, but personal distress did not predict bystander intervention. In an exploratory analysis, we tested a moderated mediation model in which personal distress moderated the relation of empathic concern to bystander intervention. The moderated mediation model was statistically significant; for students with low to moderate personal distress, empathic concern predicted self-reported intervention. However, for students high in personal distress, empathic concern was not related to self-reported intervention. The current study explained a small amount of the variance in bystander intervention. These findings demonstrate the complex family and personal factors that may explain, to a small degree, individual differences in bystander intervention. Further studies should consider the complex contextual variables that may influence this relationship.

Parental Warmth and Interpersonal Empathy as Predictors of Sexual Assault Bystander Intervention Efficacy.

Sexual victimization rates of women in the United States remain worryingly high. Much research has focused on the reduction of sexual violence with varying levels of success. One promising avenue of sexual violence reduction research provides evidence that bystanders who intervene appropriately can effectively contribute to a reduction in sexual assault. The Ecological Model for Bystander Intervention provides a conceptual framework for investigating what motivates and what inhibits bystanders. Empirical evidence on various levels of the model has been garnered; however, little is known about the association between particular developmental variables and bystander intervention efficacy situated in the microsystem. The goal of this study was to explore developmental predictors of bystander intervention efficacy. We predicted perceived parental warmth would be related to bystander intervention efficacy and that the relation would be mediated by empathy. University students (mean age = 19.2 years) completed anonymous online self-report measures of perceived maternal and paternal warmth, empathy, and bystander efficacy. The mediation model was supported by the data. As predicted, the path from parental warmth to empathy was significant (p < .001) and the path from empathy to bystander intervention efficacy was significant (p < .001). The indirect effect of parental warmth on bystander efficacy through empathy was significant (p = .001). One implication of these findings is that characteristics that are related to bystander tendencies may develop early in the family environment. Efforts to increase bystander intervention may benefit from the recognition that empathy may be founded in earlier life experiences. Limitations and future directions are discussed.

Helicopter Parenting, Authenticity, and Depressive Symptoms: A Mediation Model.

Helicopter parenting is an overinvolved, overcontrolling parenting style that likely interferes with children's healthy development of authenticity. Authenticity refers to self-awareness and a genuine expression of the self and is important for college students as they traverse the opportunities and challenges of emerging adulthood. Authenticity appears to be a protective characteristic and is negatively related to depressive symptoms. The authors proposed and tested a model in which helicopter parenting was related to depressive symptoms through authenticity. In the present investigation, college students (n = 286) completed self-report instruments assessing perceptions of helicopter parenting, current states of authenticity (self-alienation, authentic living, external influence), and depressive symptoms. Path analysis was conducted with AMOS, and the data fit the model well (χ2(1) = .580, p = .446, TLI = 1.103, CFI = 1.000, RMSEA = .000). Helicopter parenting was related to higher levels of self-alienation and external influence and lower levels of authentic living. Self-alienation and authentic living were related to depressive symptoms (external influence was not related to depressive symptoms). These findings demonstrate the importance of considering developmental predictors of authenticity and depression. Helicopter parenting is likely to inhibit authenticity in ways that may contribute to difficulties in emerging adulthood.

Non-amyloidogenic effects of α2 adrenergic agonists: implications for brimonidine-mediated neuroprotection.

The amyloid beta (Aß) pathway is strongly implicated in neurodegenerative conditions such as Alzheimer's disease and more recently, glaucoma. Here, we identify the α2 adrenergic receptor agonists (α2ARA) used to lower intraocular pressure can prevent retinal ganglion cell (RGC) death via the non-amyloidogenic Aß-pathway. Neuroprotective effects were confirmed in vivo and in vitro in different glaucoma-related models using α2ARAs brimonidine (BMD), clonidine (Clo) and dexmedetomidine. α2ARA treatment significantly reduced RGC apoptosis in experimental-glaucoma models by 97.7% and 92.8% (BMD, P<0.01) and 98% and 92.3% (Clo, P<0.01)) at 3 and 8 weeks, respectively. A reduction was seen in an experimental Aß-induced neurotoxicity model (67% BMD and 88.6% Clo, both P<0.01, respectively), and in vitro, where α2ARAs significantly (P<0.05) prevented cell death, under both hypoxic (CoCl2) and stress (UV) conditions. In experimental-glaucoma, BMD induced ninefold and 25-fold and 36-fold and fourfold reductions in Aß and amyloid precursor protein (APP) levels at 3 and 8 weeks, respectively, in the RGC layer, with similar results with Clo, and in vitro with all three α2ARAs. BMD significantly increased soluble APPα (sAPPα) levels at 3 and 8 weeks (2.1 and 1.6-fold) in vivo and in vitro with the CoCl2 and UV-light insults. Furthermore, treatment of UV-insulted cells with an sAPPα antibody significantly reduced cell viability compared with BMD-treated control (52%), co-treatment (33%) and untreated control (27%). Finally, we show that α2ARAs modulate levels of laminin and MMP-9 in RGCs, potentially linked to changes in Aß through APP processing. Together, these results provide new evidence that α2ARAs are neuroprotective through their effects on the Aß pathway and sAPPα, which to our knowledge, is the first description. Studies have identified the need for α-secretase activators and sAPPα-mimetics in neurodegeneration; α2ARAs, already clinically available, present a promising therapy, with applications not only to reducing RGC death in glaucoma but also other neurodegenerative processes involving Aß.

The retina as an early biomarker of neurodegeneration in a rotenone-induced model of Parkinson's disease: evidence for a neuroprotective effect of rosiglitazone in the eye and brain.

Parkinson's Disease (PD) is the second most common neurodegenerative disease worldwide, affecting 1 % of the population over 65 years of age. Dopaminergic cell death in the substantia nigra and accumulation of Lewy bodies are the defining neuropathological hallmarks of the disease. Neuronal death and dysfunction have been reported in other central nervous system regions, including the retina. Symptoms of PD typically manifest only when more than 70 % of dopaminergic cells are lost, and the definitive diagnosis of PD can only be made histologically at post-mortem, with few biomarkers available.In this study, a rotenone-induced rodent model of PD was employed to investigate retinal manifestations in PD and their usefulness in assessing the efficacy of a novel therapeutic intervention with a liposomal formulation of the PPAR-γ (Peroxisome proliferator-activated receptor gamma) agonist rosiglitazone.Retinal assessment was performed using longitudinal in vivo imaging with DARC (detection of apoptosing retinal cells) and OCT (optical coherence tomography) technologies and revealed increased RGCs (Retinal Ganglion Cells) apoptosis and a transient swelling of the retinal layers at day 20 of the rotenone insult. Follow-up of this model demonstrated characteristic histological neurodegenerative changes in the substantia nigra and striatum by day 60, suggesting that retinal changes precede the "traditional" pathological manifestations of PD. The therapeutic effect of systemic administration of different formulations of rosiglitazone was then evaluated, both in the retina and the brain. Of all treatment regimen tested, sustained release administration of liposome-encapsulated rosiglitazone proved to be the most potent therapeutic strategy, as evidenced by its significant neuroprotective effect on retinal neurons at day 20, and on nigrostriatal neurons at day 60, provided convincing evidence for its potential as a treatment for PD.Our results demonstrate significant retinal changes occurring in this model of PD. We show that rosiglitazone can efficiently protect retinal neurons from the rotenone insult, and that systemic administration of liposome-encapsulated rosiglitazone has an enhanced neuroprotective effect on the retina and CNS (Central Nervous System). To our knowledge, this is the first in vivo evidence of RGCs loss and early retinal thickness alterations in a PD model. Together, these findings suggest that retinal changes may be a good surrogate biomarker for PD, which may be used to assess new treatments both experimentally and clinically.

Ocular biomarkers of Alzheimer's disease.

Alzheimer's disease (AD) is a devastating neurodegenerative disease characterised clinically by a progressive decline in executive functions, memory and cognition. Classic neuropathological hallmarks of AD include intracellular hyper-phosphorylated tau protein which forms neurofibrillary tangles (NFT), and extracellular deposits of amyloid ß (Aß) protein, the primary constituent of senile plaques (SP). The gradual process of pathogenic amyloid accumulation is thought to occur 10-20 years prior to symptomatic manifestation. Advance detection of these deposits therefore offers a highly promising avenue for prodromal AD diagnosis. Currently, the most sophisticated method of 'probable AD' diagnosis is via neuroimaging or cerebral spinal fluid (CSF) biomarker analysis. Whilst these methods have reported a high degree of diagnostic specificity and accuracy, they fall significantly short in terms of practicality; they are often highly invasive, expensive or unsuitable for large-scale population screening. In recent years, ocular screening has received substantial attention from the scientific community due to its potential for non-invasive and inexpensive central nervous system (CNS) imaging. In this appraisal we build upon our previous reviews detailing ocular structural and functional changes in AD (Retinal manifestations of Alzheimer's disease, Alzheimer's disease and Retinal Neurodegeneration) and consider their use as biomarkers. In addition, we present an overview of current advances in the use of fluorescent reporters to detect AD pathology through non-invasive retinal imaging.

Real-time imaging of retinal cell apoptosis by confocal scanning laser ophthalmoscopy.

Retinal cell apoptosis occurs in many eye conditions, including glaucoma, diabetic retinopathy and Alzheimer's disease. Real-time detection of retinal cell apoptosis has potential clinical value in early disease detection, as well as evaluating disease progression and treatment efficacy. Here, we describe our novel imaging technology DARC (Detection of Apoptosing Retinal Cells), which can be used to visualize single retinal neurons undergoing apoptosis in real time, by using fluorescently labeled Annexin A5 and confocal scanning laser ophthalmoscopy (cSLO ). Clinical trials of DARC in glaucoma patients are due to start shortly, but in this chapter, we describe this technique in experimental animal models.

Romantic attachment, empathy, and the broader autism phenotype among college students.

Recent research suggests that mild autistic-like characteristics can be measured among relatives of individuals with autism and in the general population. These characteristics have been referred to as the broader autism phenotype (BAP), and include pragmatic language difficulties, aloofness, and rigidity. Evidence is growing to suggest that individuals with BAP encounter difficulties in their social interactions. Recent work demonstrates that college students scoring high on the BAP report more loneliness (Jobe & Williams White, 2007) and more interpersonal problems (Wainer, Ingersoll, & Hopwood, 2012). Because intimate relationships are important in development and are very salient in emerging adulthood, the authors examined the relation of the BAP to romantic attachment and empathy among young adults. Higher BAP scores were associated with lower empathy and higher attachment anxiety and avoidance. Specifically, pragmatic language difficulties were related to higher rates of avoidant attachment and this relationship was mediated by empathy. In contrast, pragmatic language deficits were directly related to anxious attachment.

Topical delivery of Avastin to the posterior segment of the eye in vivo using annexin A5-associated liposomes.

Effective delivery to the retina is presently one of the most challenging areas in drug development in ophthalmology, due to anatomical barriers preventing entry of therapeutic substances. Intraocular injection is presently the only route of administration for large protein therapeutics, including the anti-Vascular Endothelial Growth Factors Lucentis (ranibizumab) and Avastin (bevacizumab). Anti-VEGFs have revolutionised the management of age-related macular degeneration and have increasing indications for use as sight-saving therapies in diabetes and retinal vascular disease. Considerable resources have been allocated to develop non-invasive ocular drug delivery systems. It has been suggested that the anionic phospholipid binding protein annexin A5, may have a role in drug delivery. In the present study we demonstrate, using a combination of in vitro and in vivo assays, that the presence of annexin A5 can significantly enhance uptake and transcytosis of liposomal drug carrier systems across corneal epithelial barriers. This system is employed to deliver physiologically significant concentrations of Avastin to the posterior of the rat eye (127 ng/g) and rabbit retina (18 ng/g) after topical application. Our observations provide evidence to suggest annexin A5 mediated endocytosis can enhance the delivery of associated lipidic drug delivery vehicles across biological barriers, which may have therapeutic implications.

The potential of annexin-labelling for the diagnosis and follow-up of glaucoma.

Glaucoma is one of the leading causes of blindness in developed countries and is mainly attributable to the apoptosis of retinal ganglion cells (RGCs). Although several diagnostic tools have been developed to detect and monitor this disease, none has the requisite sensitivity to identify it at a preclinical stage or to perceive small changes in retinal health over short periods. Specifically, irreversible visual changes occur before neuronal damage is discovered. The most widely accepted in vitro assay for apoptotic cells involves the use of fluorescent annexin A5. The radiolabelling of this marker makes it possible to assess, in vivo and non-invasively, various diseases in which the apoptotic process is pivotal, such as myocardial infarction or tumour response to chemotherapy. Recently, a new technique has been developed to visualise directly individual RGCs undergoing apoptosis in the living eye. This DARC (detection of apoptosing retinal cells) technology uses fluorescently labelled annexin A5 to bind apoptosing retinal neurons and confocal scanning laser ophthalmoscopy to detect the marked dying cells. Based on experimental models, DARC has been suggested to offer a direct and quantitative assessment of the retinal condition of patients. A Phase I clinical trial in glaucoma patients is scheduled to start shortly. This technology has the potential to pre-empt the diagnosis of glaucoma prior to visual deterioration, to provide an accurate numeric evaluation highlighting even small retinal changes and to allow the rapid judgement of the efficacy of both current and new therapeutic strategies.

The efficacy of an intimate partner violence prevention program with high-risk adolescent girls: a preliminary test.

This study examined the efficacy of a brief (four session) intimate partner violence (IPV) prevention program (Building a Lasting Love, Langhinrichsen-Rohling et al. 2005) that was designed to reduce the relationship violence of predominantly African American inner-city adolescent girls (n = 72) who were receiving teen pregnancy services. These high-risk girls were randomly assigned to the prevention program (n = 39) or waitlist control (n = 33) conditions. Implementation fidelity was documented. As predicted, girls who successfully completed the program (n = 24) reported significant reductions in their perpetration of psychological abuse toward their baby's father as compared to the control (n = 23) participants. They also reported experiencing significantly less severe IPV victimization over the course of the program. Preliminary analyses indicated that avoidant attachment to one's partner may be associated with less program-related change. These findings support the contention that brief IPV prevention programs can be targeted to selected groups of high-risk adolescents.