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Stability of phosphorothioate oligonucleotides in aqueous ammonia in presence of stainless steel.
Turney, B J; Cheruvallath, Z S; Andrade, M; Cole, D L; Ravikumar, V T.
Nucleosides Nucleotides ; 18(1): 89-93, 1999 Jan.
Article in English | MEDLINE | ID: mdl-10048225

ABSTRACT

Oligodeoxyribonucleotide phosphorothioates undergo desulfurization in presence of metal in aqueous ammonia at elevated temperatures. The extent of sulfur loss and chain cleavage have been investigated.


Subject(s)
Ammonia , Oligodeoxyribonucleotides/chemistry , Stainless Steel , Thionucleotides/chemistry , Base Sequence , Drug Stability , Glass , Nuclear Magnetic Resonance, Biomolecular , Water
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