[Histoenzymatic characteristics of the articular cartilage in ontogeny and in osteoarthrosis deformans]. / Gistoénzimaticheskaia kharakteristika sustavnogo khriashcha v ontogeneze i pri deformiruiushchem osteoartroze.

Enzymo-histochemical study of the human joint cartilage in ontogenesis showed a high 5-nucleotidase activity at all stages of human development. Alkaline phosphatase was detectable only in the zones of enchondral ossification of the joint cartilage and in the ossification "nuclei", while in adults only in the chondrocytes over the ossification line. Osteocytes of the subchondral bone contain neither 5-nucleotidase nor alkaline phosphatase, the latter being detected in the endosteal cells only. The damage of the joint cartilage in the osteoarthrosis results in the redistribution of the osteo- and chondrogenesis enzymes with their localization in the zones of the regenerative reconstruction of the subchondral bone tissue.

[Method of detecting alkaline phosphatase and 5-nucleotidase in histological bone sections]. / K metodike vyiavleniia shchelochnoi fosfatazy i 5-nukleotidazy v gistologicheskikh srezakh kostnoi tkani.

Modification of the methods of Gomory, Pearce, and Reis for the detection of alkaline phosphatase and 5-nucleotidase in the bone tissue is described. The authors used fixation of the bone tissue in acetone at a temperature of 0 to 4 degrees C for 6 to 12 hours, decalcification in Grip's fluid followed by cutting in a freezing microtome of incompletely decalcified bone material and its decalcification in sections. As a result, localization of the enzyme is detected by the distribution of black sediments of cobalt sulfide.

[Pappenheim method of panoptic staining of histological sections]. / K metodike panopticheskoi okraski gistologicheskikh srezov po Pappengeimu.

A new method of azure-eosin staining of histological preparations during fixation with a 10% formalin solution, as well as of the cartilaginous and medullar tissues after decalcification in the 10% trilon B solution, pH 7,3 or in the de Castro liquid is described. This method has a number of advantages over the method os hematoxylin-eosin staining: it presents not only a clear idea about all cellular elements but also allows one to stain mast cells, fungi, microbes, Rickettsia, mucus, fibrin, necrobiotic changes in cells. Differentiation of hemopoietic cells and elements of reticuloendothelium, histiocytes is possible in the hemopoietic tissue. The method is accessible and simple and does not require special equipment.