ABSTRACT
We aimed to evaluate the membrane expression of DcR1 and DcR2 in the normal endometrium (NE), endometrial atypical hyperplasia (EAH) and endometrioid endometrial cancer (EEC). The study comprised 101 patients: 20 NE, 14 EAH and 67 EEC. Membrane expression of DcR1 and DcR2 was examined and presented as total score (TS). The membrane expression of both DcR1 and DcR2 was more common in EEC than in NE (p < 0.001; p < 0.001). A strong correlation was found between type of endometrial tissue (NE/EAH/EEC) and the TS of DcR1 (p = 0.001) and DcR2 (p < 0.001). In EEC, the TS of DcR1 and DcR2 was not related to grading and survival. The TS of DcR1 negatively correlated with staging (p = 0.018), but DcR2 did not. The membrane expression of decoy receptors for TRAIL DcR1 and DcR2 is greater in NE than EEC. In EEC patients, membrane expression of DcR1 and DcR2 are not independent predictors of survival.
Subject(s)
Carcinoma, Endometrioid/metabolism , Endometrial Hyperplasia/metabolism , Endometrial Neoplasms/metabolism , Endometrium/metabolism , Tumor Necrosis Factor Decoy Receptors/metabolism , Case-Control Studies , Female , GPI-Linked Proteins/metabolism , Humans , Receptors, Tumor Necrosis Factor, Member 10cABSTRACT
To assess membrane expression of DR4 and DR5 in the normal endometrium (NE), endometrial atypical hyperplasia (EAH) and endometrioid endometrial cancer (EEC), the study examined 101 patients: 20 NE, 14 EAH and 67 EEC. The expression of DR4 and DR5 was examined and presented as the total score (TS). DR4 expression was seen in 18 NE, 11 EAH and 10 EEC. DR5 expression was seen in 20 NE, 13 EAH and 21 EEC. A strong correlation between type of endometrial tissue and TS of both receptors was identified. In EEC TS of DR4 and DR5 was not related to grading, staging or survival. Malignant transformation in the endometrium is related to reduction of membrane DR4 and DR5 expression. The level of membrane staining of the receptors in EEC is not dependent on grading and staging, and is not sufficient to predict survival in EEC patients.
Subject(s)
Carcinoma, Endometrioid/metabolism , Endometrial Hyperplasia/metabolism , Endometrial Neoplasms/metabolism , Receptors, TNF-Related Apoptosis-Inducing Ligand/metabolism , Case-Control Studies , Female , HumansABSTRACT
The tissue microarray (TMA) method currently is not used to render a primary diagnosis of cancer, but its scientific value has been proved in studies of various cancer types. TMA technology still is not used often for uterine tumors, however. We investigated the repeatability of histological diagnosis of endometrioid endometrial cancer (EEC) using conventional histology and TMA using 2 mm cores. We examined EEC tissues from 171 patients. Formalin fixed, paraffin embedded tissue donor blocks from EEC specimens were selected and examined histologically. Duplicate 2 mm tissue cores were inserted into a TMA recipient block. EEC tissues were examined as hematoxylin-eosin stained sections from the TMAs. EEC tissue was identified in the TMAs in 158 cases (92.4%) and not found in 13 cases (7.6%). On the TMA slides, both EEC positive cores were identified in 129 cases (75.4%), but only one core in 29 cases (17.0%). Among 342 biopsies of the donor blocks (each case in duplicate), EEC was found in 287 cases (83.9%) using the TMA: 124/146 (84.9%) with superficial infiltration, 153/178 (86.0%) with deep myometrial infiltration, and 10/18 (55.6%) without myometrial infiltration. We concluded that two 2 mm tissue cores from a biopsy of a donor block inserted into a TMA recipient block were sufficient to diagnose EEC in more than 90% of cases. EEC was identified in the TMAs with similar frequency with respect to superficial and deep myometrial infiltration. Cases without myometrial infiltration were identified less often.
Subject(s)
Endometrial Neoplasms/pathology , Tissue Array Analysis/methods , Endometrial Neoplasms/diagnosis , Female , Histocytological Preparation Techniques/instrumentation , Histocytological Preparation Techniques/methods , Humans , Paraffin Embedding/methods , Quality Control , Reproducibility of Results , Tissue Array Analysis/instrumentationABSTRACT
In our report we present the use of the Vecchietti method in our modification to form an artificial vagina in women with Rokitansky-Küster-Hauser syndrome. Five surgical procedures were carried out and expected therapeutic result was obtained in the all cases. This method was estimated as very safe. No side effects were observed.
Subject(s)
Abnormalities, Multiple/surgery , Uterus/abnormalities , Vagina/abnormalities , Vagina/surgery , Vulva/abnormalities , Adult , Female , Humans , Syndrome , Treatment Outcome , Vulva/surgeryABSTRACT
The studies material comprised 10 placentae from pregnancies at risk for premature labour with delayed intrauterine growth of the fetus treated with Salbupart from the 32nd week of pregnancy. In placental homogenates the biochemical components were determined for fragmentary assessment of carbohydrate and lipid metabolism and production on high-energy compounds. Salbupart was found to increase the oxidation processes in the placenta. This increases the production of biologically useful chemical energy which is expressed as increased energy charge (so called Atkinson's index or adenylate energy charge). Increased energy yield of the placenta results in improved materno-fetal transport and contributes to greater weight gain of the hypotrophic fetus.
