[State of corneal nerve fibers after laser-assisted in situ keratomileusis]. / Sostoyanie nervnykh volokon rogovitsy posle lazernogo keratomileza in situ.

PURPOSE: To assess the state of corneal nerve fibers (CNF) based on quantitative parameters after laser correction of myopia using LASIK. MATERIAL AND METHODS: The study examined 141 eyes - 80 eyes with myopia and 61 emmetropic eyes. In 47 cases a microkeratome was used to form a corneal flap, and in 33 cases a femtosecond laser was used (LASIK and FemtoLASIK, respectively). Confocal microscopy was performed before, as well as 1, 3, and 6 months after correction. Objective analysis of CNF involved automatic calculation of the anisotropy (KΔL) and symmetry (Ksym) coefficients of CNF orientation in the Liner 1.2S software. RESULTS: Lower values of KΔL were revealed in myopia as compared to emmetropia. Regardless of the method of flap formation, no CNF could be detected in the central cornea 1 month after myopia correction. In all cases after FemtoLASIK and in 2 cases after LASIK, CNF in the central cornea were first visualized after 3 months, and after 6 months CNF could be detected in all cases. At a similar observation time, CNF were visualized both after LASIK and after FemtoLASIK in the superior and inferior peripheral portions of the cornea. Analysis of long-term postoperative changes in KΔL and Ksym revealed a decrease in the former and an increase in the latter. CONCLUSION: Evaluation of the state of CNF after corneal refractive correction should take into account the initial changes in CNF in myopia that are possibly associated with an increase in axial eye length. A more dynamic restoration of innervation was noted in cases where the flap was formed with the femtosecond laser, which may be explained by the fact that the mechanism of tissue separation when using this type of coherent radiation is gentler. Considering the changes in the quantitative indicators characterizing the state of CNF, the question of «completeness¼ of the restored CNF as a result of reinnervation remains open.

[The effect of the tear film on the results of a direct assessment of the sensitivity of the cornea]. / Vliyanie sleznoi plenki na rezul'taty pryamoi otsenki chuvstvitel'nosti rogovitsy.

Attempts to find clinical application for esthesiometry are associated with studying sensitivity disorders that occur in various diseases (diabetes mellitus, keratitis of various etiologies, corneal erosion, dry eye syndrome), as well as after surgical interventions. PURPOSE: To evaluate the influence of the tear film on the results of direct assessment of corneal sensitivity. MATERIAL AND METHODS: The study was conducted in a group of 20 healthy volunteers. Criteria for inclusion in the group: absence of any ocular and general systemic diseases in the anamnesis, normal indicators of tear production and stability of the tear film according to Schirmer and Norn tests. The process of testing involved a specially designed device that was used to exert a dosed force by jets of air on the frontal corneal surface and the pressure was recorded at the moment of tactile sensations and at the breakup time of tear film, as an indicator of its biomechanical stability. RESULTS: A strong correlation have been found between the pressure of the air stream at the moment of getting tactile sensations and at the breakup of the tear film (R=0.91, p=0.0094). The obtained data indicate that the biomechanical resistance of the lacrimal film is a significant factor preventing the correct assessment of the results of direct corneal esthesiometry in the range of values close to conditionally normal sensitivity. CONCLUSION: In this regard, in their current technical implementation, modern esthesiometers can only be used for monitoring (not for diagnosis!) of pathological processes by analyzing relative (compared to each other) - rather than absolute - sensitivity measures (optimally in combination with analysis of the structural changes in the corneal nerves based on laser confocal microscopy).

[Condition of corneal nerve fibers after laser keratorefractive surgery]. / Sostoianie nervnykh volokon rogovitsy posle lazernykh keratorefraktsionnykh operatsii.

The article reviews studies dedicated to evaluation of corneal nerve fibers (CNF) after various laser keratorefractive methods of correcting refractive error based on confocal microscopy. The matter of studying the dynamics of the condition of CNF after laser keratorefractive techniques continues to be relevant and requires further research with regard to the prevalence of refractive errors on the one hand, and the future life of patients who undergo these procedures, on the other. Certain heterogeneity of the research results presented in this review can be partially explained by the subjective nature of the study algorithm (in particular, the need for manual tracing of nerve fibers) and the lack of objective quantitative indicators of CNF condition suitable for statistical processing.