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1.
Int J Obes (Lond) ; 42(3): 535-541, 2018 03.
Article in English | MEDLINE | ID: mdl-29151595

ABSTRACT

BACKGROUND/OBJECTIVES: Expanding visceral adiposity is associated with increased inflammation and increased risk for developing obesity-related comorbidities. The goal of this study was to examine high fat diet (HFD)-induced differences in adipocyte size and cytokine/chemokine expression in visceral and subcutaneous adipose depots in obesity-prone (OP) and obesity-resistant (OR) rats. METHODS: OP and OR rats were fed either a low fat diet (LFD, 10% kilocalories from fat) or HFD (60% kilocalories from fat) for 7 weeks. Adipocyte size and the presence of crown-like structures in epididymal and inguinal adipose tissue were determined. A multiplex cytokine/chemokine panel was used to assess the expression of inflammatory markers in epididymal and inguinal adipose tissues. RESULTS: A higher percentage of large adipocytes (>5000 µm2) was detected in the epididymal and inguinal adipose tissues of OP rats and a higher percentage of small adipocytes (<4000 µm2) was detected in the epididymal and inguinal adipose tissues of OR rats. More crown-like structures were identified in epididymal adipose tissue of OP rats fed a LFD, compared to OR rats. Consumption of a HFD increased the number of crown-like structures in OR, but not OP rats. Epididymal expression of pro-inflammatory cytokines (IL-1ß and TNF-α) was higher in OP rats, compared to OR rats fed LFD. HFD consumption increased epididymal expression of GM-CSF, IL-1α, IL-1ß, IL-6, MIP-2 and TNF-α in OP and OR rats. Inguinal expression of pro-inflammatory cytokines (IL-1α, IL-1ß and TNF-α) was higher in OP rats, compared to OR rats. CONCLUSIONS: Overall, these data suggest that a higher susceptibility to developing obesity is characterized by large adipocytes and increased visceral adipose inflammation. Interestingly, in OR rats, the detrimental effects of HFD consumption on visceral adipose inflammation are evident with only small increases in weight and adiposity, suggesting that HFD also increases the risk for obesity-related comorbidities in OR rats.


Subject(s)
Adipocytes/metabolism , Diet, High-Fat/adverse effects , Inflammation/metabolism , Obesity/metabolism , Adipose Tissue/cytology , Adipose Tissue/metabolism , Animals , Biomarkers/analysis , Biomarkers/metabolism , Cells, Cultured , Cytokines/analysis , Cytokines/metabolism , Epididymis/cytology , Epididymis/metabolism , Male , Rats
2.
Sci Rep ; 6: 19991, 2016 Jan 29.
Article in English | MEDLINE | ID: mdl-26822337

ABSTRACT

Solution-processed, non-toxic carbon dots (CDs) have attracted much attention due to their unique photoluminescence (PL) properties. They are promising emissive layers for flexible light-emitting devices. To this end, the CDs in pristine aqueous solutions need to be transferred to form solid-state thin films without sacrificing their original PL characteristics. Unfortunately, solid-state PL quenching induced by extra non-radiative (NR) energy transfer among CDs would significantly hinder their practical applications in optoelectronics. Here, a facile, low-cost and effective method has been utilized to fabricate high-performance CD/polymer light-emitting flexible films with submicron-structured patterns. The patterned polymers can serve as a solid matrix to disperse and passivate CDs, thus achieving high internal quantum yields of 61%. In addition, they can act as an out-coupler to mitigate the waveguide-mode losses, approximately doubling the external light-extraction efficiency. Such CD/polymer composites also exhibit good photo-stability, and thus can be used as eco-friendly, low-cost phosphors for solid-state lighting.

3.
J Colloid Interface Sci ; 459: 175-182, 2015 Dec 01.
Article in English | MEDLINE | ID: mdl-26291573

ABSTRACT

Magnetic nanoparticles are the next tool in medical diagnoses and treatment in many different biomedical applications, including magnetic hyperthermia as alternative treatment for cancer and bacterial infections, as well as the disruption of biofilms. The colloidal stability of the magnetic nanoparticles in a biological environment is crucial for efficient delivery. A surface that can be easily modifiable can also improve the delivery and imaging properties of the magnetic nanoparticle by adding targeting and imaging moieties, providing a platform for additional modification. The strategy presented in this work includes multiple nitroDOPA anchors for robust binding to the surface tied to the same polymer backbone as multiple poly(ethylene oxide) chains for steric stability. This approach provides biocompatibility and enhanced stability in fetal bovine serum (FBS) and phosphate buffer saline (PBS). As a proof of concept, these polymer-particles complexes were then modified with a near infrared dye and utilized in characterizing the integration of magnetic nanoparticles in biofilms. The work presented in this manuscript describes the synthesis and characterization of a nontoxic platform for the labeling of near IR-dyes for bioimaging.


Subject(s)
Biofilms , Dihydroxyphenylalanine/chemistry , Fluorescent Dyes/chemistry , Legionella pneumophila , Nanoparticles/chemistry , Polyethylene Glycols/chemistry , Animals , Cattle , Legionella pneumophila/cytology , Legionella pneumophila/physiology , Mice , Microscopy, Fluorescence
4.
Cancer Nanotechnol ; 4(1-3): 13-20, 2013.
Article in English | MEDLINE | ID: mdl-26316898

ABSTRACT

Urinary tract infection (UTI) is a predominant condition in prostate cancer patients. Escherichia coli ORN178 (EC-178) is the uropathogen that causes recurrent infection by binding specifically to adhesins of prostate cancer cells (DU-145 cells). Gold nanoparticles (GNPs) have been used in biodiagnosis of pathogens. In this study, we have investigated the binding time of EC-178 to DU-145 cells, the cytotoxicity and uptake of plain and mannose functionalized and 20 and 200 nm GNPs (d-mannan (Mn)-GNPs). We also investigated the protein corona of GNPs when incubated with fetal bovine serum to study the protein corona which decides the biological fate of the GNPs. It was seen that EC-178 binds and is inside the DU-145 cells by 3 h of incubation period. Plain 20 nm GNPs decrease the percentage of viable cells in 48 and 72 h in log and lag phase of DU-145 cells. It was also observed that the Mn-GNPs were taken up by the DU-145 cells significantly more than the plain GNPs. Protein corona was observed when GNPs were incubated with fetal bovine serum which was confirmed by dynamic light scattering measurements and SDS-PAGE gel.

5.
Fitoterapia ; 82(2): 230-6, 2011 Mar.
Article in English | MEDLINE | ID: mdl-20951188

ABSTRACT

Cytochrome P450 (CYP) enzyme inhibitory properties of six chromenylated amide compounds (CAs) from Amyris plumieri are described. Inhibition of CYP microsomes (CYP1A1, CYP1A2, CYP1B1, CYP2D6, CYP3A4 and CYP2C19) was monitored using a fluorescent assay. Potent inhibition was found against CYP1A1 with IC(50) and K(i) for CA1 (acetamide), being the lowest at 1.547 ± 1.0 µM and 0.37 µM respectively, displaying non-competitive kinetics. The selectivity for CYP1A1 was increased in CA3 (butanamide), which also exhibited cytotoxicity against breast cancer cells, MCF7 with an IC(50) of 47.46 ± 1.62 µM. Structure-activity relationship studies provide insight at a molecular level for CAs with implications in chemoprevention and chemotherapy.


Subject(s)
Amides/therapeutic use , Antineoplastic Agents, Phytogenic/therapeutic use , Breast Neoplasms/drug therapy , Cytochrome P-450 CYP1A1/antagonists & inhibitors , Phytotherapy , Plant Extracts/therapeutic use , Rutaceae/chemistry , Acetamides/isolation & purification , Acetamides/pharmacology , Acetamides/therapeutic use , Amides/isolation & purification , Amides/pharmacology , Antineoplastic Agents, Phytogenic/isolation & purification , Antineoplastic Agents, Phytogenic/pharmacology , Benzopyrans/isolation & purification , Benzopyrans/pharmacology , Benzopyrans/therapeutic use , Enzyme Inhibitors/isolation & purification , Enzyme Inhibitors/pharmacology , Enzyme Inhibitors/therapeutic use , Female , Humans , Plant Extracts/isolation & purification , Plant Extracts/pharmacology , Structure-Activity Relationship
6.
Oncogene ; 28(37): 3286-95, 2009 Sep 17.
Article in English | MEDLINE | ID: mdl-19597463

ABSTRACT

Lung cancer is a leading cause of cancer death due to the high incidence of metastasis; therefore, novel and effective treatments are urgently needed. A current strategy is cancer-specific targeted gene therapy. Although many identified that cancer-specific promoters are highly specific, they tend to have low activity compared with the ubiquitous cytomegalovirus (CMV) promoter, limiting their application. We developed a targeted gene therapy expression system for lung cancer that is highly specific with strong activity. Our expression vector uses the survivin promoter, highly expressed in many cancers but not normal adult tissues. We enhanced the survivin promoter activity comparable to the CMV promoter in lung cancer cell lines using an established platform technology, whereas the survivin promoter remained weak in normal cells. In mouse models, the transgene was specifically expressed in the lung tumor tissue, compared with the CMV promoter that was expressed in both normal and tumor tissues. In addition, the therapeutic gene BikDD, a mutant form of pro-apoptotic Bcl2 interacting killer, induced cell killing in vitro, and inhibited cell growth and prolonged mouse survival in vivo. Importantly, there was virtually no toxicity when BikDD was expressed with our expression system. Thus, the current report provides a therapeutic efficacy and safe strategy worthy of development in clinical trials treating lung cancer.


Subject(s)
Apoptosis Regulatory Proteins/genetics , Apoptosis Regulatory Proteins/therapeutic use , Genetic Therapy/methods , Lung Neoplasms/pathology , Lung Neoplasms/therapy , Membrane Proteins/genetics , Membrane Proteins/therapeutic use , Mutant Proteins/genetics , Mutant Proteins/therapeutic use , Animals , Cell Line, Tumor , Cell Proliferation , Clinical Trials as Topic , Cytomegalovirus/genetics , Humans , Inhibitor of Apoptosis Proteins , Lung Neoplasms/genetics , Male , Mice , Microtubule-Associated Proteins/genetics , Mitochondrial Proteins , Neoplasm Transplantation , Nucleic Acid Amplification Techniques , Promoter Regions, Genetic/genetics , Survival Rate , Survivin , Time Factors
7.
Horm Metab Res ; 39(3): 173-8, 2007 Mar.
Article in English | MEDLINE | ID: mdl-17373630

ABSTRACT

In an attempt to clarify the role of phosphatase and tensin homologue deleted on chromosome 10 (PTEN) in muscle insulin resistance, we investigated the effect of PTEN on phosphoinositide 3 (PI3)-kinase/Akt related insulin signaling pathway in skeletal muscle-like C2C12 cells damaged by tumor necrosis factor-alpha (TNFalpha). C2C12 cells cultured with TNFalpha (10 ng/ml) for 1 h displayed a marked decrease of insulin-stimulated 2-[14C]-deoxy-D-glucose (2-DG) uptake in parallel with an elevation of PTEN mRNA and protein levels. However, pretreatment of PTEN antisense oligonucleotide (AS) (1 micromol/l for 3 days) for specific inhibition of PTEN expression in C2C12 cells abolished the TNFalpha-induced changes in 2-DG uptake. Similar pretreatment with PTEN AS, but not with sense oligonucleotide (1 micromol/l for 3 days), eliminated the ability of TNFalpha to impair insulin-stimulated signals including p85 regulatory subunit of PI3-kinase expression and the degree of Akt serine phosphorylation as well as protein expression in glucose transporter subtype 4. Data taken from cultured C2C12 cells emphasize the negative regulatory of muscle PI3-kinase/Akt signaling pathways as the major substrate of PTEN but also support the concept that PTEN contributes to the development of insulin resistance in skeletal muscle.


Subject(s)
Insulin/metabolism , Muscle Fibers, Skeletal/drug effects , Muscle Fibers, Skeletal/metabolism , PTEN Phosphohydrolase/metabolism , Signal Transduction/drug effects , Tumor Necrosis Factor-alpha/pharmacology , Tumor Suppressor Proteins/metabolism , Animals , Cell Line , Gene Expression Regulation/drug effects , Glucose/metabolism , Glucose Transporter Type 4/metabolism , Mice , Oligonucleotides, Antisense/pharmacology , PTEN Phosphohydrolase/genetics , Phosphatidylinositol 3-Kinases/metabolism , Proto-Oncogene Proteins c-akt/metabolism , RNA, Messenger/genetics , RNA, Messenger/metabolism , Tumor Suppressor Proteins/genetics
8.
Diabetes Obes Metab ; 9(1): 39-49, 2007 Jan.
Article in English | MEDLINE | ID: mdl-17199717

ABSTRACT

AIMS: We investigated the mechanism(s) by which valsartan, a selective antagonist of angiotensin subtype 1 (AT(1)) receptor, decreased plasma glucose in streptozotocin (STZ)-induced diabetic rats. METHODS: The plasma glucose concentration was assessed by the glucose oxidase method. The concentration of beta-endorphin in plasma or medium incubating adrenal medulla was measured using an enzyme-linked immunosorbent assay. The mRNA levels of the subtype 4 form of glucose transporter (GLUT4) in soleus muscle and phosphoenolpyruvate carboxykinase (PEPCK) in the liver were detected by Northern blotting analysis, while the protein levels of GLUT4 in isolated soleus muscle and hepatic PEPCK were investigated using Western blotting analysis. RESULTS: A single intravenous injection of valsartan dose-dependently increased plasma beta-endorphin-like immunoreactivity (BER) in parallel with the lowering of plasma glucose concentration in STZ-induced diabetic rats. Naloxone and naloxonazine inhibited the plasma glucose-lowering action of valsartan at doses sufficient to block opioid micro-receptors. In contrast to its action in wild-type diabetic mice, valsartan failed to modify plasma glucose in opioid micro-receptor knockout diabetic mice. Bilateral adrenalectomy in STZ-induced diabetic rats eliminated both the plasma glucose-lowering action and the plasma BER-elevating action of valsartan. In the isolated adrenal medulla of STZ-induced diabetic rats, angiotensin II (Ang II) or valsartan did not affect spontaneous BER secretion. Activation of cholinergic receptors by 1.0 micromol/l acetylcholine (ACh) enhanced BER secretion from the isolated adrenal medulla of STZ-induced diabetic rats, but not in the presence of 1.0 nmol/l Ang II, while valsartan reversed this inhibition by Ang II in a concentration-dependent manner. Treatment of STZ-induced diabetic rats with valsartan (0.2 mg/kg) three times daily for 3 days resulted in an increase in gene expression of GLUT4 in soleus muscle and impeded the reduction of elevated mRNA or protein level of hepatic PEPCK. Both of these effects were blocked by opioid micro-receptor antagonist. CONCLUSIONS: The results suggest that blockade of AT(1) receptor by valsartan may enhance the adrenal beta-endorphin secretion induced by ACh, activating the opioid micro-receptors to increase glucose utilization and/or to decrease hepatic gluconeogenesis, resulting in the reduction of plasma glucose in STZ-induced diabetic rats.


Subject(s)
Angiotensin II Type 1 Receptor Blockers/pharmacology , Blood Glucose/drug effects , Diabetes Mellitus, Experimental/blood , Tetrazoles/pharmacology , Valine/analogs & derivatives , Adrenal Medulla/drug effects , Adrenal Medulla/physiopathology , Adrenalectomy , Animals , Blood Glucose/metabolism , Diabetes Mellitus, Experimental/chemically induced , Diabetes Mellitus, Experimental/drug therapy , Gene Expression Regulation/drug effects , Glucose Transporter Type 4/genetics , Glucose Transporter Type 4/metabolism , Glutathione Peroxidase/genetics , Glutathione Peroxidase/metabolism , Liver/metabolism , Male , Mice , Mice, Knockout , Muscle, Skeletal/drug effects , Muscle, Skeletal/metabolism , RNA, Messenger/genetics , Rats , Rats, Wistar , Receptors, Opioid, mu/antagonists & inhibitors , Receptors, Opioid, mu/genetics , Streptozocin , Tissue Culture Techniques , Valine/pharmacology , Valsartan , beta-Endorphin/blood , beta-Endorphin/metabolism
9.
Rheumatology (Oxford) ; 45(10): 1230-7, 2006 Oct.
Article in English | MEDLINE | ID: mdl-16595513

ABSTRACT

OBJECTIVES: Systemic lupus erythematosus (SLE) is characterized by the presence of autoantibodies (autoAbs) directed against the nuclear structure. Previous studies have demonstrated that dendritic cells (DCs) can process and present self-antigens (Ags) from apoptotic cells (ACs) in lupus. However, there is no direct evidence demonstrating that ACs provide self-Ags, such as histones, to stimulate autoreactive T-cells in lupus. METHODS: AC-pulsed bone marrow-derived DCs (AC-BMDCs) were used to stimulate autoreactive T-cells in vitro and in vivo. RESULTS: In our study, we found that AC-BMDCs could induce the proliferation of CD4+ T-cells from unprimed NZB x NZW F1 (BWF1) mice, which spontaneously develop SLE, but not CD4+ T-cells, from non-autoimmune DBA-2 x NZW F1 (DWF1) mice. In addition, AC-BMDCs could induce significant proliferative responses to certain histone peptide-specific T-cells. Furthermore, these AC-BMDCs could induce a considerable anti-DNA Ab response in vivo after adoptive transfer into DWF1 mice, suggesting that AC-BMDCs can break tolerance in normal mice and initiate an autoimmune response. CONCLUSION: Our study provides a direct link between self-epitopes from ACs presented by DCs and autoreactive T-cell activation, and demonstrates that ACs are critical for the induction of autoimmunity in vivo.


Subject(s)
Adoptive Transfer/methods , CD4-Positive T-Lymphocytes/immunology , Dendritic Cells/transplantation , Lupus Erythematosus, Systemic/therapy , Lymphocyte Activation , Animals , Apoptosis , Autoantibodies/immunology , Autoimmunity , Cell Line , Cell Proliferation , Cytokines/immunology , DNA/immunology , Female , Flow Cytometry , Immunoglobulin G/immunology , Kidney Glomerulus/immunology , Mice , Mice, Mutant Strains , Models, Animal , Phagocytosis
10.
Diabetologia ; 48(7): 1386-92, 2005 Jul.
Article in English | MEDLINE | ID: mdl-15959754

ABSTRACT

AIMS/HYPOTHESIS: This study investigated the role of opioid mu-receptor activation in the improvement of insulin resistance. METHODS: Myoblast C2C12 cells were cultured with IL-6 to induce insulin resistance. Radioactive 2-deoxyglucose (2-DG) uptake was used to evaluate the effect of loperamide on insulin-stimulated glucose utilisation. Protein expression and phosphorylation in insulin-signalling pathways were detected by immunoblotting. RESULTS: The insulin-stimulated 2-DG uptake was reduced by IL-6. Loperamide reversed this uptake, and the uptake was inhibited by blockade of opioid mu-receptors. Insulin resistance induced by IL-6 was associated with impaired expression of the insulin receptor (IR), IR tyrosine autophosphorylation, IRS-1 protein content and IRS-1 tyrosine phosphorylation. Also, an attenuated p85 regulatory subunit of phosphatidylinositol 3-kinase, Akt serine phosphorylation and the protein of glucose transporter subtype 4 were observed in insulin resistance. Loperamide reversed IL-6-induced decrement of these insulin signals. CONCLUSIONS/INTERPRETATION: Opioid mu-receptor activation may improve IL-6-induced insulin resistance through modulation of insulin signals to reverse the responsiveness of insulin. This provides a new target in the treatment of insulin resistance.


Subject(s)
Insulin/physiology , Interleukin-6/pharmacology , Loperamide/pharmacology , Receptors, Opioid, mu/physiology , Signal Transduction/physiology , Animals , Biological Transport , Cell Line , Deoxyglucose/pharmacokinetics , Insulin/pharmacology , Mice , Naloxone/pharmacology , Phosphoproteins/drug effects , Phosphoproteins/metabolism , Phosphorylation , Receptors, Opioid, mu/drug effects , Signal Transduction/drug effects
11.
Diabetologia ; 48(4): 767-75, 2005 Apr.
Article in English | MEDLINE | ID: mdl-15756537

ABSTRACT

AIMS/HYPOTHESIS: The present study investigated the effect of agmatine, an endogenous ligand of imidazoline receptors, on plasma glucose in streptozotocin-induced diabetic rats (STZ-diabetic rats). METHODS: Plasma glucose was assessed by the glucose oxidase method. Plasma insulin and beta-endorphin-like immunoreactivity in plasma or adrenal medulla were measured by enzyme-linked immunosorbent assay. Systolic blood pressure was determined by the tail-cuff method. The mRNA levels of glucose transporter subtype 4 (GLUT4) in soleus muscle and phosphoenolpyruvate carboxykinase (PEPCK) in liver were detected by northern blotting. Protein levels of GLUT4 in soleus muscle and hepatic PEPCK were estimated using western blotting analysis. RESULTS: After intravenous injection into fasting STZ-diabetic rats for 30 min, agmatine decreased plasma glucose in a dose-dependent manner without changing systolic blood pressure. At the same time, plasma beta-endorphin-like immunoreactivity also increased in STZ-diabetic rats receiving the same treatment. Plasma glucose was significantly elevated in STZ-diabetic rats by an intravenous injection of clonidine at a dose sufficient to decrease systolic blood pressure. Involvement of I(1)-imidazoline receptors and/or alpha2-adrenoceptors in this effect of agmatine was thus unlikely. The lowering of plasma glucose and increase of plasma beta-endorphin-like immunoreactivity by agmatine were abolished by pretreating the rats with BU-224 at a dose sufficient to block I(2)-imidazoline receptors. Both effects of agmatine were also abolished in adrenalectomised STZ-diabetic rats. Moreover, agmatine enhanced beta-endorphin-like immunoreactivity release from the isolated adrenal medulla of STZ-diabetic rats, an effect also blocked by BU-224. Release of beta-endorphin from the adrenal glands by I(2)-imidazoline receptor activation seems responsible for the plasma glucose-lowering action of agmatine. This was supported by the fact that intravenous injection of naloxone or naloxonazine at doses sufficient to block opioid mu-receptors inhibited the action of agmatine. In addition to lowering plasma glucose, repeated intravenous injection of agmatine into STZ-diabetic rats for 4 days also increased mRNA and protein levels of GLUT4 in soleus muscle. The same treatment also reversed the higher mRNA and protein levels of PEPCK in liver of STZ-diabetic rats. CONCLUSIONS/INTERPRETATION: Our results suggest that agmatine may activate I(2)-imidazoline receptors in the adrenal gland. This enhances secretion of beta-endorphin, which can activate opioid mu-receptors to increase GLUT4 gene expression and/or suppress hepatic PEPCK gene expression, resulting in a lowering of plasma glucose in diabetic rats lacking insulin. The results provide a potential new target for intervention in type 1 diabetes.


Subject(s)
Adrenal Glands/drug effects , Blood Glucose/metabolism , Diabetes Mellitus, Experimental/drug therapy , Receptors, Drug/agonists , Adrenal Glands/metabolism , Adrenal Medulla/drug effects , Adrenal Medulla/metabolism , Adrenalectomy , Agmatine/administration & dosage , Agmatine/pharmacology , Animals , Blood Pressure/drug effects , Clonidine/pharmacology , Diabetes Mellitus, Experimental/blood , Diabetes Mellitus, Experimental/metabolism , Gene Expression/drug effects , Glucose Transporter Type 4 , Imidazoles/pharmacology , Imidazoline Receptors , Male , Monosaccharide Transport Proteins/genetics , Muscle Proteins/genetics , Phosphoenolpyruvate Carboxykinase (GTP)/genetics , Rats , Rats, Wistar , Receptors, Drug/antagonists & inhibitors , Receptors, Opioid, mu/antagonists & inhibitors , beta-Endorphin/blood
12.
Clin Exp Pharmacol Physiol ; 30(10): 734-8, 2003 Oct.
Article in English | MEDLINE | ID: mdl-14516411

ABSTRACT

1. Loperamide has an ability to lower the plasma glucose concentration in streptozotocin (STZ)-induced diabetic rats. In the present study, we investigated the molecular mechanisms by which loperamide regulates plasma glucose concentrations in the absence of insulin. 2. Loperamide, at a dose sufficient (17.6 microg/kg) to activate mu-opioid receptors, significantly decreased plasma glucose levels in STZ-diabetic rats. The mRNA and protein levels of glucose transporter 4 (GLUT-4) in soleus muscle, detected by northern and western blotting, respectively, were increased after repeated intravenous administration of loperamide (17.6 micro g/kg) to STZ-diabetic rats over 3 days. Moreover, similar treatment with loperamide (17.6 microg/kg) for 3 days reversed the elevated mRNA and protein levels of phosphoenolpyruvate carboxykinase (PEPCK) in the liver of STZ-diabetic rats to near the levels seen in normal rats. 3. These results suggest that activation of mu-opioid receptors by loperamide can increase glucose utilization in peripheral tissues and/or reverse the higher gene expression of PEPCK to inhibit hepatic gluconeogenesis, thereby lower plasma glucose in diabetic rats lacking insulin.


Subject(s)
Blood Glucose/metabolism , Diabetes Mellitus, Experimental/blood , Diabetes Mellitus, Experimental/drug therapy , Loperamide/therapeutic use , Muscle Proteins , Animals , Glucose Transporter Type 4 , Loperamide/pharmacology , Male , Monosaccharide Transport Proteins/biosynthesis , Protein Serine-Threonine Kinases/metabolism , RNA, Messenger/biosynthesis , Rats , Rats, Wistar
13.
Diabetes Metab Res Rev ; 19(3): 209-15, 2003.
Article in English | MEDLINE | ID: mdl-12789654

ABSTRACT

BACKGROUND: Adenosine A1 receptor (A1-AR) activation can lower plasma glucose in diabetic rats lacking insulin. We investigated the change in A1-AR gene expression in diabetic rats. METHODS: The incorporation of [U-(14)C]-glucose into glycogen was carried out to evaluate the effect of N(6)-cyclopentyladenosine (CPA) on glucose utilization in vitro. The plasma glucose concentration was assessed by the glucose oxidase method. The mRNA and protein levels of A1-AR in isolated liver were detected by Western blotting analysis and Northern blotting analysis, respectively. RESULTS: The effect of CPA, an agonist of A1-AR, on glycogen incorporation in hepatocytes isolated from streptozotocin-induced diabetic rats (STZ-diabetic rats) was more marked than that from the normal rats. However, similar glycogen synthesis was not modified by 12-O-tetradecanoylphorbol-13-acetate (TPA), an activator of protein kinase C, in the isolated hepatocytes from both the normal and the STZ-diabetic rats. A change in response at the receptor level can thus be considered. The mean level of liver mRNA transcripts encoding A1-AR was increased in STZ-diabetic rats to about 250% of that in normal rats. Exogenous insulin at a dose sufficient to normalize the plasma glucose of STZ-diabetic rats reversed the mRNA level of A1-AR in the liver after a four-day treatment. Similar results were also observed in STZ-diabetic rats that received treatment with phlorizin for four days. Moreover, the protein level of A1-AR was higher in the liver of STZ-diabetic rats than that in the normal rats. Similar treatment with exogenous insulin or phlorizin reversed the elevated protein level of A1-AR in the liver of STZ-diabetic rats to near the normal level. Therefore, correction of hyperglycemia in STZ-diabetic rats can reverse the higher gene expression of A1-AR in liver. CONCLUSIONS: The obtained results suggest that an increase in plasma glucose is responsible for the higher gene expression of A1-AR in the liver of STZ-diabetic rats.


Subject(s)
Adenosine/analogs & derivatives , Adenosine/pharmacology , Diabetes Mellitus, Experimental/physiopathology , Gene Expression Regulation/genetics , Hepatocytes/metabolism , Liver/physiopathology , Receptor, Adenosine A1/genetics , Animals , Blood Glucose/drug effects , Blood Glucose/metabolism , Diabetes Mellitus, Experimental/blood , Gene Expression Regulation/drug effects , Glucose/metabolism , Hepatocytes/drug effects , Insulin/pharmacology , Liver/drug effects , Liver Glycogen/biosynthesis , Purinergic P1 Receptor Agonists , Rats , Reference Values , Tetradecanoylphorbol Acetate/pharmacology
14.
Horm Metab Res ; 35(4): 251-8, 2003 Apr.
Article in English | MEDLINE | ID: mdl-12778369

ABSTRACT

The role of alpha 1A -adrenoceptors in the regulation of opioid secretion from the adrenal glands of streptozotocin-induced diabetic rats (STZ-diabetic rats) was examined in an attempt to determine the mechanism of plasma glucose-lowering action of caffeic acid. In agreement with a previous report, we showed that caffeic acid produced a dose-dependent lowering of the plasma glucose concentration in STZ-diabetic rats along with an increase of plasma beta-endorphin-like immunoreactivity (BER). These actions of caffeic acid were abolished by pretreatment with WB 4101 or RS 17 056 at doses sufficient to block alpha 1A -adrenoceptors. In addition, naloxone and naloxonazine at doses effective for blocking opioid micro -receptors abolished the plasma glucose-lowering action of caffeic acid. Also, unlike that in wild-type diabetic mice, caffeic acid failed to produce a plasma glucose lowering effect in opioid micro -receptor knockout diabetic mice. We observed that caffeic acid could enhance BER release from isolated rat adrenal medulla in a concentration-dependent manner; inhibitors of alpha 1A -adrenoceptors such as WB 4101 and RS 1705 abolished this action. Investigations of the signal pathways further supported that activation of alpha 1A -adrenoceptor is responsible for the stimulatory effect of caffeic acid on BER secretion from the adrenal medulla. In the presence of U73312, a specific inhibitor of phospholipase C, the caffeic acid-induced increase of BER was reduced in a concentration-dependent manner, but it was not affected by U73343, the negative control of U73312. Chelerythrine and GF 109203X also diminished the action of caffeic acid at concentrations sufficient for inhibiting protein kinase C. Moreover, bilateral adrenalectomy in STZ-diabetic rats resulted in the loss of this plasma glucose-lowering effect of caffeic acid, and there was no increase in plasma BER with caffeic acid. Therefore, beta-endorphin release from the adrenal gland appears to be responsible for the lowering of plasma glucose in STZ-diabetic rats induced by caffeic acid, through the activation of alpha 1A -adrenoceptors.


Subject(s)
Blood Glucose/metabolism , Caffeic Acids/pharmacology , Diabetes Mellitus, Experimental/metabolism , Receptors, Adrenergic, alpha-1/metabolism , beta-Endorphin/pharmacology , Adrenalectomy , Adrenergic alpha-1 Receptor Antagonists , Analysis of Variance , Animals , Caffeic Acids/metabolism , Enzyme-Linked Immunosorbent Assay , Male , Mice , Mice, Knockout , Rats , Rats, Wistar , Receptors, Opioid, mu/metabolism , Type C Phospholipases/antagonists & inhibitors , beta-Endorphin/metabolism
15.
Horm Metab Res ; 34(10): 570-6, 2002 Oct.
Article in English | MEDLINE | ID: mdl-12439785

ABSTRACT

The effect of beta-endorphin on plasma glucose levels was investigated in streptozotocin-induced diabetic rats (STZ-diabetic rats). A dose-dependent lowering of plasma glucose was observed in the fasting STZ-diabetic rat fifteen minutes after intravenous injection of beta-endorphin. The plasma glucose-lowering effect of beta-endorphin was abolished by pretreatment with naloxone or naloxonazine at doses sufficient to block opioid mu-receptors. Also, unlike wild-type diabetic mice, beta-endorphin failed to induce its plasma glucose-lowering effect in the opioid mu-receptor knock-out diabetic mice. In isolated soleus muscle, beta-endorphin enhanced the uptake of radioactive glucose in a concentration-dependent manner. Stimulatory effects of beta-endorphin on glycogen synthesis were also seen in hepatocytes isolated from STZ-diabetic rats. The blockade of these actions by naloxone and naloxonazine indicated the mediation of opioid mu-receptors. In the presence of U73312, the specific inhibitor of phospholipase C (PLC), the uptake of radioactive glucose into isolated soleus muscle induced by beta-endorphin was reduced in a concentration-dependent manner, but it was not affected by U73343, the negative control of U73312. Moreover, chelerythrine and GF 109203X diminished the stimulatory action of beta-endorphin on the uptake of radioactive glucose at a concentration sufficient to inhibit protein kinase C (PKC). The data obtained suggest that activating opioid mu-receptors by beta-endorphin may increase glucose utilization in peripheral tissues via the PLC-PKC pathway to lower plasma glucose in diabetic rats lacking insulin.


Subject(s)
Blood Glucose/metabolism , Diabetes Mellitus, Experimental/blood , Naloxone/analogs & derivatives , beta-Endorphin/pharmacology , Animals , Diabetes Mellitus, Experimental/drug therapy , Diabetes Mellitus, Experimental/metabolism , Enzyme Inhibitors/pharmacology , Glucose/pharmacokinetics , Hepatocytes/drug effects , Hepatocytes/metabolism , In Vitro Techniques , Male , Muscle, Skeletal/drug effects , Muscle, Skeletal/metabolism , Naloxone/pharmacology , Narcotic Antagonists/pharmacology , Rats , Rats, Wistar , Receptors, Opioid, mu/deficiency , Receptors, Opioid, mu/genetics , Receptors, Opioid, mu/metabolism , beta-Endorphin/antagonists & inhibitors
16.
Clin Exp Pharmacol Physiol ; 29(5-6): 419-22, 2002.
Article in English | MEDLINE | ID: mdl-12010186

ABSTRACT

1. The present study compared the activity of catechol- O-methyltransferase (COMT) in the liver and plasma of streptozotocin (STZ)-induced diabetic rats with that in normal rats. The activity of COMT was estimated by the metabolism of noradrenaline to metanephrine (MN), both measured by high-performance liquid chromatography with electrochemical detection. 2. Formation of MN was decreased in the liver of STZ- diabetic rats compared with normal rats. The amount of MN was also decreased in plasma obtained from STZ-diabetic rats. A Michaelis-Menten plot showed a reduction in the maximum velocity and an increase in the Km for COMT in liver samples from STZ-diabetic rats. 3. The role of hyperglycaemia in the lowering of COMT activity was then investigated using phlorizin or insulin at doses sufficient to normalize plasma glucose from STZ-diabetic rats. Both insulin and phlorizin treatment of STZ-diabetic rats for 4 days restored the activity of COMT to that seen in normal rats. Thus, correction of hyperglycaemia in STZ-diabetic rats can reverse the decrease in COMT activity. The activity of COMT is lower in STZ-diabetic rats than in normal rats mainly due to the higher plasma glucose.


Subject(s)
Catechol O-Methyltransferase/metabolism , Diabetes Mellitus, Experimental/metabolism , Liver/enzymology , Animals , Blood Glucose/metabolism , Catechol O-Methyltransferase/blood , Diabetes Mellitus, Experimental/enzymology , In Vitro Techniques , Insulin/pharmacology , Male , Metanephrine/blood , Metanephrine/metabolism , Phlorhizin/pharmacology , Rats , Rats, Wistar , Streptozocin
17.
Diabetes ; 50(12): 2815-21, 2001 Dec.
Article in English | MEDLINE | ID: mdl-11723065

ABSTRACT

The effect of tramadol on the plasma glucose level of streptozotocin (STZ)-induced diabetic rats was investigated. A dose-dependent lowering of plasma glucose was seen in the fasting STZ-induced diabetic rats 30 min after intravenous injection of tramadol. This effect of tramadol was abolished by pretreatment with naloxone or naloxonazine at doses sufficient to block opioid mu-receptors. However, response to tramadol was not changed in STZ-induced diabetic rats receiving p-chlorophenylalanine at a dose sufficient to deplete endogenous 5-hydroxytrptamine (5-HT). Therefore, mediation of 5-HT in this action of tramadol is ruled out. In isolated soleus muscle, tramadol enhanced the uptake of radioactive glucose in a concentration-dependent manner. The stimulatory effects of tramadol on glycogen synthesis were also seen in hepatocytes isolated from STZ-induced diabetic rats. The blockade of these actions by naloxone and naloxonazine indicated the mediation of opioid mu-receptors. The mRNA and protein levels of the subtype 4 form of glucose transporter in soleus muscle were increased after repeated treatments for 4 days with tramadol in STZ-induced diabetic rats. Moreover, similar repeated treatments with tramadol reversed the elevated mRNA and protein levels of phosphoenolpyruvate carboxykinase in the liver of STZ-induced diabetic rats. These results suggest that activation of opioid mu-receptors by tramadol can increase the utilization of glucose and/or decrease hepatic gluconeogenesis to lower plasma glucose in diabetic rats lacking insulin.


Subject(s)
Blood Glucose/metabolism , Diabetes Mellitus, Experimental/blood , Hypoglycemic Agents/pharmacology , Muscle Proteins , Naloxone/analogs & derivatives , Narcotics/pharmacology , Tramadol/pharmacology , Animals , Diabetes Mellitus, Experimental/drug therapy , Dose-Response Relationship, Drug , Gluconeogenesis , Glucose/metabolism , Glucose Transporter Type 4 , Glycogen/biosynthesis , Hypoglycemic Agents/administration & dosage , Injections, Intravenous , Kinetics , Liver/drug effects , Liver/enzymology , Male , Monosaccharide Transport Proteins/analysis , Monosaccharide Transport Proteins/genetics , Muscle, Skeletal/chemistry , Muscle, Skeletal/drug effects , Muscle, Skeletal/metabolism , Naloxone/pharmacology , Narcotic Antagonists/pharmacology , Narcotics/administration & dosage , Phosphoenolpyruvate Carboxykinase (GTP)/chemistry , Phosphoenolpyruvate Carboxykinase (GTP)/genetics , RNA, Messenger/analysis , Rats , Rats, Wistar , Receptors, Opioid, mu/physiology , Tramadol/administration & dosage
18.
Plant Cell Physiol ; 42(10): 1156-68, 2001 Oct.
Article in English | MEDLINE | ID: mdl-11673632

ABSTRACT

Lily MADS box gene 1 (LMADS1), with sequence homology to the AP3 family of genes, was cloned and characterized from lily (Lilium longiflorum). LMADS1 protein contains almost complete consensus sequence of the PISTILLATA (PI)-derived motif (YEFRVQPSQPNLH) found in the AP3 family of genes and paleoAP3 motif (YGSHDLRLA) found in the AP3 family of genes from the low eudicot, magnolid dicot and monocot species. LMADS1 mRNA was expressed in all four whorls of the flower and absent in the vegetative leaves. The LMADS1 protein was only detected in the petals and stamens, indicating that LMADS1 is possibly post-transcriptionally regulated in lily. Arabidopsis plants transformed with 35S::LMADS1 produced flowers with short petals and stamens, however, no floral organ conversion was observed. Ectopic expression of LMADS1 cDNA truncated with the MADS box domain in Arabidopsis generated the ap3-like dominant negative mutation in which the petals were converted into sepal-like structures and the stamens were converted into carpel-like structures. Yeast two-hybrid analysis indicated that LMADS1 truncated with the MADS box domain is able to sufficiently interact with the Arabidopsis PI protein. This result supports that LMADS1 is the functional counterpart of the AP3 gene in lily. Interestingly, in contrast to other B functional genes, LMADS1 truncated with the MADS box domain is able to strongly form homodimers. LMADS1 may represent an ancestral form of the B function gene, which retains the ability to form homodimers in regulating petal and stamen development in lily.


Subject(s)
Arabidopsis Proteins , Arabidopsis/genetics , Genes, Dominant , Genes, Plant , Homeodomain Proteins/genetics , Lilium/genetics , MADS Domain Proteins/genetics , Mutation , Transcription Factors/genetics , Amino Acid Sequence , Base Sequence , DNA Primers , Lilium/ultrastructure , MADS Domain Proteins/chemistry , Microscopy, Electron, Scanning , Molecular Sequence Data , Phylogeny , Reverse Transcriptase Polymerase Chain Reaction
19.
Kaohsiung J Med Sci ; 17(6): 294-301, 2001 Jun.
Article in English | MEDLINE | ID: mdl-11559967

ABSTRACT

In this study, we investigated the prevalence of chronic complications, including nephropathy and retinopathy, in patients newly diagnosed as type 2 diabetes mellitus. All hyperglycemic subjects were recruited into our study when they visited the outpatient department at Kaohsiung Medical University Hospital over a one-year period. These subjects had fasting plasma glucose higher than 140 mg/dl, or plasma glucose higher than 200 mg/dl in the 2nd hour during an oral glucose tolerance test. Among 148 patients registered as newly diagnosed type 2 diabetes mellitus, 18.2% of the patients had nephropathy, noted by measuring their urine albumin excretion rate and daily protein loss, and 25.5% had retinopathy, noted by fundoscope and fluorescent angiography. The age of overt proteinuric patients (41.5 +/- 3.4 yrs) was significantly younger than those without nephropathy (51.8 +/- 1.0 yrs). Systolic and diastolic blood pressure was significantly higher in patients with microalbuminuria (142.4 +/- 6.0/88.8 +/- 2.6 mmHg) and overt proteinuria (153.8 +/- 13.6/96.8 +/- 9.5 mmHg) than normoalbuminuric patients (128.3 +/- 2.3/81.9 +/- 1.1 mmHg). There was no significant difference in cholesterol, triglyceride, HbA1C, sex or body mass index among normoalbuminric, microalbuminuric, or overt proteinuric patients. The severity of retinopathy was parallel with the severity of nephropathy. Based on our results, chronic diabetic complications, including nephropathy and retinopathy, may occur even when diabetes is newly diagnosed. It is necessary to look for complications, especially in newly documented diabetic patients who are young and hypertensive.


Subject(s)
Diabetes Mellitus, Type 2/complications , Diabetic Nephropathies/epidemiology , Diabetic Retinopathy/epidemiology , Hypertension/epidemiology , Age Factors , Female , Humans , Male , Middle Aged , Prevalence
20.
Horm Metab Res ; 33(8): 467-71, 2001 Aug.
Article in English | MEDLINE | ID: mdl-11544560

ABSTRACT

Opioids play an important role in the regulation of glucose homeostasis. In the previous report, we showed that activation of opioid mu-receptors produced a plasma glucose lowering effect in diabetic rats lacking insulin. In the present study, we found that the response of opioid mu-receptor is more sensitive in streptozotocin-induced diabetic rats (STZ-diabetic rats) than in normal rats. Intravenous injection of loperamide, an agonist of opioid mu-receptors, induced a dose-dependent decrease of plasma glucose from 3 microg/kg to 60 microg/kg in fasting STZ-diabetic rats. However, loperamide decreased the plasma glucose of normal fasting rats at the doses of 0.3 mg/kg to 1.5 mg/kg, which were much higher than those needed to produce the same effect in diabetic rats. The plasma glucose-lowering action of loperamide at the dose effective in normal rats disappeared in opioid mu-receptor knockout mice, while the plasma glucose-lowering response to loperamide was still observed in wild-type mice. This opens the possibility of mediation through opioid mu-receptor in the plasma glucose-lowering action of loperamide. Moreover, the mRNA level of opioid mu-receptor in the liver markedly increased in STZ-diabetic rats compared to normal rats. Normalization of plasma glucose concentrations in STZ-diabetic rats with exogenous insulin or phlorizin reversed mRNA and protein levels of opioid mu-receptor in the liver after 4 days of treatment. This shows that correction of hyperglycemia in STZ-diabetic rats may reverse the higher gene expression of opioid mu-receptor. These results suggest that hyperglycemia is responsible for increase of opioid mu-receptor in STZ-diabetic rats.


Subject(s)
Blood Glucose/metabolism , Diabetes Mellitus/metabolism , Loperamide/pharmacology , Receptors, Opioid, mu/genetics , Receptors, Opioid, mu/metabolism , Animals , Blood Glucose/drug effects , Diabetes Mellitus/chemically induced , Dose-Response Relationship, Drug , Gene Expression/drug effects , Hyperglycemia/metabolism , Injections, Intravenous , Insulin/metabolism , Insulin/pharmacology , Loperamide/metabolism , Male , Mice , Mice, Knockout , Narcotics/pharmacology , RNA, Messenger/agonists , RNA, Messenger/antagonists & inhibitors , Rats , Rats, Wistar , Receptors, Opioid, mu/agonists , Receptors, Opioid, mu/antagonists & inhibitors , Streptozocin
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