ABSTRACT
The quantities of hormones and hormone-like materials (histamine, dopamine, epinephrine, diiodotyrosine and corticosterone, in the following collectively: hormones) incorporated by the lymphocytes of neonatal rats were several orders greater than those taken up by the lymphocytes of adult rats. Uptake tended to decrease by one week of age, but it was still 2-3 times greater than in adulthood. It fell to a minimum at two weeks to one month of age, then it increased slightly by 2 or 6-8 months of age.
Subject(s)
Hormones/metabolism , Lymphocytes/metabolism , Amino Acids/metabolism , Animals , Cell Membrane/metabolism , Growth , In Vitro Techniques , Rats , T-Lymphocytes/metabolism , Time FactorsABSTRACT
Tetrahymena pyriformis GL cells and rat thymic lymphocytes equally showed intranuclear incorporation of triiodothyronine, diiodotyrosine, histamine, serotonin, epinephrine and corticosterone--used as control--on exposure to labelled hormones. All hormones appeared in the nucleus in a predominantly heterochromatic localization. Lymphocytes, although smaller in size, incorporated more hormone than Tetrahymena. The intranuclear accumulation of corticosterone did not differ between Tetrahymena and thymocytes, and was high in both relative to total incorporation by the cell. Autoradiographically detected localizations did not notably vary with the type of cell or hormone.
Subject(s)
Amino Acids/metabolism , Cell Nucleus/metabolism , Hormones/metabolism , Lymphocytes/metabolism , Tetrahymena pyriformis/metabolism , Animals , Corticosterone/metabolism , Dopamine/metabolism , Epinephrine/metabolism , Histamine/metabolism , Male , Rats , Rats, Inbred Strains , Serotonin/metabolism , T-Lymphocytes/metabolismABSTRACT
Incorporation of 3H-uridine by RNA in Tetrahymena was differently influenced by insulin, glucagon, follicle-stimulating hormone (FSH), thyrotropic hormone (TSH), adrenocorticotropic hormone (ACTH) and chorion-gonadotropic hormone (PMSG). TSH caused it to increase considerably and durably after an initial depression, while glucagon caused it to rise over the control throughout. Insulin, and especially PMSG, depressed the incorporation of label considerably, the latter to 3-6% of the control value by 120 min. ACTH and FSH accounted for an initial depression of RNA synthesis which, however, returned to normal 30 min after treatment. Remarkably, while the chemically similar hormones acted differently, insulin and glucagon showed the same trend of positive and negative influence, respectively.
