ABSTRACT
Enchytraeus ohtakai sp. nov. (Enchytraeidae, Clitellata, Oligochaeta) was discovered in the organic matter of a wastewater treatment facility of a plums processing plant in Honshu, Japan. The wastewater is characterized by high organic matter content and low salt concentration. Morphological analysis and DNA-sequencing of a fragment of the COI barcoding gene show that the new species belongs to the E. albidus species group. Within this group it differs in: vasa deferentia restricted to XII, preclitellar bundles with mostly three chaetae, postclitellar bundles with two or three, dorsal blood vessel from XII or XIII, spermathecal ectal duct completely glandular. spermatheca with a large diverticulum, accessory sexual glands present in XII, clitellum ventrally almost absent. The individual gene trees of COI analysis recovered this new species as a monophyletic group within the genus Enchytraeus, closely related to E. albidus species group.
Subject(s)
Oligochaeta , Animals , Japan , Oligochaeta/classification , Oligochaeta/genetics , Prunus domestica , Sewage , Manufacturing and Industrial FacilitiesABSTRACT
Four genera of Philopotamidae (Trichoptera) are known from Japan: Chimara, Dolophilodes, Kisaura, and Wormaldia. Among them, adults of the genus Kisaura are known in the Oriental and East Palearctic region; but until now, larvae of this genus have not been observed. In this study, molecular evidence is presented to support the relationship between adults and larvae of Kisaura minakawai Arefina 2005 from hyporheic zones of Japanese rivers. Morphological features of the larva of K. minakawai, which include very small eyes and somewhat dorsoventrally flattened (depressed) head capsules, compared with those of other philopotamid species, are also suggested to be adapted to the hyporheic zone. Detailed morphology of K. minakawai larval characters are provided.
