ABSTRACT
The effect of ambiocor (15 mg/100 ml), which contains natural substrates of energy metabolism, on the contractility of papillary muscles (PM) of the right ventricle of the rat heart was studied at stimulation frequencies from 0.1 to 3.0 Hz at a temperature of 30 +/- 1 degrees C (n = 7). The effect was recorded 20 min after the addition of the preparation. It was demonstrated that ambiocor causes a significant (about 70%), independent of stimulation frequency, suppression of the amplitude of isometric contractions (negative inotropic effect), which is coupled with an increase in the relative value of the rest potentiation effect (a qualitative index of calcium content in sarcoplasmic reticulum). The influence of the mixture leads to significant alterations in the time parameters of the "contraction-relaxation" cycle: an increase in the duration of latent period; and a decrease in the time to peak tension and half-relaxation time (TR50%). The effect of the mixture is partially reversible. During the washing of the preparation with the control solution, the qualitative indicators of the contractile activity of papillary muscles are substantially improved in comparison with the initial ones. The character of alterations allows one to assume that the effect of ambiocor in the papillary muscles of the rat heart is realized partly through the suppression of the activity of sarcolemmal calcium channels.
Subject(s)
Heart Rate/drug effects , Myocardial Contraction/drug effects , Myocardium/metabolism , Animals , Aspartic Acid/pharmacology , Calcium/metabolism , Drug Combinations , Energy Metabolism , Fumarates/pharmacology , Glutamates/pharmacology , Homeostasis , Male , Muscle Relaxation , Papillary Muscles/drug effects , Papillary Muscles/physiology , Rats , Succinates/pharmacology , Tartrates/pharmacology , Taurine/pharmacologyABSTRACT
Menopausal transition is often accompanied by a variety of adverse pathological symptoms, currently treated with hormone replacement therapy, which is associated with a number of health risks. This report investigated the role of a food supplement--a composition of energy-exchange metabolites, with succinate as the main component--for treating menopausal syndrome. We studied the impact of a 4-week succinate-based food composition (SBC) treatment on the estral cycle, and bone mass and calcium content of aging mice. The impact of SBC on hormone levels and on the progression of several neurovegetative and psycho-emotional symptoms was further investigated in a randomized, double-blind, placebo-controlled clinical study of early menopausal women. Data were collected from questionnaires, Kupperman index scores, Spielberger-Hanin tests, and blood analysis of hormone levels taken at baseline and throughout the 5-week study. A "rejuvenating" effect of SBC on menopausal animals was observed, expressed as restoration of the estral cycle and an increase in the weight and calcium content of bone tissue. Furthermore, in the randomized, placebo-controlled clinical study in menopausal women, SBC-based monotherapy significantly lowered most subjectively evaluated characteristics of menopausal syndrome and increased blood serum levels of estradiol fourfold. This monotherapy also alleviated symptoms of some neurovegetative and psycho-emotional disorders, such as hot flushes, headache, and anxiety. Succinate-based therapy alleviated many biochemical symptoms of menopause in aging mice and early menopausal women, as well as neurovegetative and psycho-emotional disorders in women. Succinate-based therapy appeared to be free of adverse side effects.
Subject(s)
Aging , Dietary Supplements , Menopause , Succinic Acid/therapeutic use , Adult , Aging/drug effects , Aging/metabolism , Aging/psychology , Animals , Autonomic Nervous System/drug effects , Bone and Bones/drug effects , Bone and Bones/metabolism , Calcium/metabolism , Double-Blind Method , Estradiol/blood , Estrogen Replacement Therapy , Estrous Cycle/drug effects , Female , Follicle Stimulating Hormone/blood , Humans , Luteinizing Hormone/blood , Menopause/blood , Menopause/metabolism , Menopause/psychology , Mice , Mice, Inbred Strains , Middle Aged , Succinic Acid/administration & dosage , Succinic Acid/adverse effects , Syndrome , Treatment OutcomeABSTRACT
Clinical placebo-controlled study of Enerlit-Clima (bioactive succinate-based food additive) a showed positive effect of the preparation on general clinical and psychoemotional manifestations of the climacteric syndrome. A trend to an increase in estradiol level in early pathological climacteric and normalization of the endometrial status were observed.
Subject(s)
Climacteric/drug effects , Food Additives/therapeutic use , Succinates/therapeutic use , Adult , Anxiety/drug therapy , Blood Glucose/drug effects , Depression/drug therapy , Female , Food Additives/pharmacology , Fumarates/pharmacology , Fumarates/therapeutic use , Glutamine/pharmacology , Glutamine/therapeutic use , Glycine/pharmacology , Glycine/therapeutic use , Gonadal Steroid Hormones/blood , Hot Flashes/drug therapy , Humans , Lipids/blood , Middle Aged , Sleep Initiation and Maintenance Disorders/drug therapy , Succinates/pharmacology , Tocopherols , alpha-Tocopherol/analogs & derivatives , alpha-Tocopherol/pharmacology , alpha-Tocopherol/therapeutic useABSTRACT
The substrate composition containing succinic acid, substrates for succinate synthesis, and antioxidants increased the efficiency of pharmacotherapy in patients with coronary heart disease and hypertension. We revealed an increase in the quality of life and decrease in the functional class of heart failure, incidence of arrhythmias and exercise-related pain, and blood cholesterol concentration.
Subject(s)
Cardiovascular Diseases/drug therapy , Cardiovascular Diseases/metabolism , Energy Metabolism , Adult , Aged , Aged, 80 and over , Arrhythmias, Cardiac/drug therapy , Arrhythmias, Cardiac/metabolism , Cholesterol/blood , Coronary Disease/drug therapy , Coronary Disease/metabolism , Drug Therapy, Combination , Exercise Test , Female , Heart Diseases/drug therapy , Heart Diseases/metabolism , Heart Failure/drug therapy , Heart Failure/metabolism , Humans , Hypertension/drug therapy , Hypertension/metabolism , Isotonic Solutions/metabolism , Male , Middle Aged , Myocardial Ischemia/complications , Myocardial Ischemia/metabolism , Physical Fitness , Quality of Life , Substrate SpecificityABSTRACT
We propose a method of evaluation of anticlimacteric efficiency of a drug by its effect on the estrous cycle. The study was carried out on 9-month-old mice with retained, but notably reduced reproductive function. Analysis of the cell components of the estrous cycle was carried out on histological preparations of vaginal smears.
