ABSTRACT
Two typical storage proteins (prolamin and glutelin) in a total of 14 samples of ancient rice cultivars were examined for their accumulation in the endosperms during days after flowering (DAF), extraction efficiency with various solvents, and variations in polypeptide components or amino acid compositions. There was little difference in accumulation profiles between this and previous observations, in which glutelin appeared on 5-7 DAF and prolamin on 7-9 DAF. As for the extraction of prolamin and glutelin, it was most effectively attained by sequential usage of 55% propanol and 21% SDS. As a result of SDS-PAGE, prolamin and glutelin proved to be composed mainly of a single polypeptide with a molecular weight (MW) of 15 kDa and two subunits with MWs of 21 kDa (alpha) and 32 kDa (beta), respectively. A comparison between the ancient and modern rice cultivars revealed the same physicochemical properties in either case of prolamin or glutelin. Additionally, a good relationship was observed on the total protein and glutelin (but not prolamin) contents in the ancient rice cultivar.
Subject(s)
Glutens/chemistry , Oryza/chemistry , Plant Proteins/chemistry , Electrophoresis, Polyacrylamide Gel , Glutens/isolation & purification , Japan/epidemiology , Molecular Weight , Oryza/classification , Plant Proteins/isolation & purification , Prolamins , Propanols/administration & dosageABSTRACT
Foxtail millet glutelin 60 kDa (MG60) was purified by preparative SDS-PAGE, and the N-terminal amino acid sequence was determined within 20 residues. The result demonstrated that the primary structure at N-terminal of MG60 was almost identical to those of the granule-bound starch synthase (GBSS) proteins from rice, barley, corn, wheat and potato. The existence of common epitopes among MG60 and GBSS proteins from these starch-storing cereals were corroborated by immunoblot analysis using antisera raised against MG60. These facts strongly suggest a close relationship between MG60-like glutelins and GBSS proteins.
Subject(s)
Edible Grain/chemistry , Glutens/chemistry , Starch Synthase/chemistry , Amino Acid Sequence , Electrophoresis, Gel, Two-Dimensional , Electrophoresis, Polyacrylamide Gel , Hordeum/chemistry , Immunochemistry/methods , Molecular Weight , Oryza/chemistry , Panicum/chemistry , Triticum/chemistry , Zea mays/chemistryABSTRACT
Immunochemical relationships between salt-soluble proteins (albumins plus globulins) from buckwheat and indigo seeds were shown by immunoblot and immunodiffusion analyses using rabbit antisera raised against buckwheat globulins. These antigenic crossreactivities were roughly consistent with their polypeptide components judged by two-dimensional electrophoresis and SDS-PAGE analyses.
Subject(s)
Globulins/immunology , Animals , Cross Reactions , Immunohistochemistry , Plants , Poaceae , Rabbits , Seeds/immunologyABSTRACT
A speech audiometric trial is described for testing the degree of hearing loss and its pathogenesis. Test vowels and distorted speech sounds were synthesized with DECtalk for use in speech audiometric tests. Results showed that 100% articulation can be obtained with undistorted synthesized vowels, while reasonable articulation scores can be obtained with distorted synthetic speech signals. This method could solve some of the problems inherent in use of recorded natural Japanese speech sounds as acoustic stimuli during otological examinations.
