Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 15 de 15
Filter
Add more filters










Publication year range
1.
Clin Exp Dermatol ; 38(8): 897-903, 2013 Dec.
Article in English | MEDLINE | ID: mdl-24252082

ABSTRACT

BACKGROUND: The Kv1.3 voltage-gated potassium channel is selectively upregulated upon activation in effector memory T (TEM ) cells in inflamed tissue, and plays an important role in maintenance of T-cell activation. Although Kv1.3 blockers have been shown to ameliorate allergic contact dermatitis (ACD) in a rat model, it remains unknown whether the effect of Kv1.3 blockers on ACD is mediated by suppressing TEM cell function and/or whether naive T-cells or central memory T (TCM ) cells are influenced. AIM: To analyse the detailed mechanism of Kv1.3 blockers in a rat model of ACD. METHODS: We examined the effects of a Kv1.3 blocker on inflammation and production of the effector cytokine interferon (IFN)-γ in inflamed tissue in rat ACD. Single-cell suspensions were isolated from inflamed rat ears (TEM cells), and regional lymph nodes (naive T/TCM cells), and the effect of Kv1.3 blockers on anti-CD3-stimulated IFN-γ production in vitro was measured. RESULTS: The Kv1.3 blocker significantly suppressed ear inflammation and IFN-γ production at the protein level in vivo. It also suppressed in vitro IFN-γ production from TEM cells from inflamed tissues, but did not suppress the function of naive T/TCM cells from lymph nodes. CONCLUSIONS: We found that the Kv1.3 blocker ameliorated ACD by inhibiting TEM cell functions only, thus Kv1.3 blockers could be a potentially selective therapeutic agent for TEM cell-mediated inflammatory skin diseases without producing harmful side-effects.


Subject(s)
Dermatitis, Allergic Contact/drug therapy , Ficusin/pharmacology , Immunologic Memory/drug effects , Kv1.3 Potassium Channel/antagonists & inhibitors , Potassium Channel Blockers/pharmacology , T-Lymphocytes/drug effects , Animals , Cells, Cultured , Dermatitis, Allergic Contact/immunology , Dermatitis, Allergic Contact/metabolism , Disease Models, Animal , Ear , Female , Interferon-gamma/metabolism , Kv1.3 Potassium Channel/physiology , Lymph Nodes/cytology , Rats
2.
Reproduction ; 125(5): 667-75, 2003 May.
Article in English | MEDLINE | ID: mdl-12713429

ABSTRACT

Primordial germ cells are important cells for the study of germ cell lineage. It has proved difficult to obtain highly purified primordial germ cells for preparation of a specific antibody. In the present study, a new method for purifying mouse primordial germ cells was developed using a Nycodenz gradient. Furthermore, the polyclonal anti-mouse primordial germ cells IgG derived from mouse primordial germ cells was prepared. As this IgG reacted only with primordial germ cells obtained at day 12.5 after mating, this antibody appeared to recognize the stage-specific antigen of primordial germ cells. One reason that a continuous primordial germ cell marker has not been obtained is because the purity of the primordial germ cells used has been too low to prepare the antibody. This new method represents a significant improvement in the purification of primordial germ cells; it is simpler than previous methods, and produced mouse primordial germ cells with a purity of more than 95%. In addition, the separation reagent Nycodenz is non-toxic and achieved separation of primordial germ cells without attachment of antibodies against the primordial germ cell membrane surface. This new purification method and stage-specific antibody will be useful for the analysis of the mechanisms of primordial germ cell migration.


Subject(s)
Germ Cells/cytology , Animals , Antibodies, Monoclonal , Cell Cycle , Cell Lineage , Cell Separation/methods , Contrast Media , Female , Germ Cells/immunology , Immunoglobulin G/immunology , Iohexol , Mice , Microscopy, Electron, Scanning
3.
J Hum Genet ; 46(10): 566-71, 2001.
Article in English | MEDLINE | ID: mdl-11587069

ABSTRACT

Glutamine:fructose-6-phosphate amidotransferase (GFAT1) is the rate-limiting enzyme in the hexosamine biosynthetic pathway, which plays an important role in hyperglycemia-induced insulin resistance. To evaluate the role of GFAT1 expression, we analyzed the expression profiles of GFAT1 mRNA in various human tissues using reverse transcriptase-polymerase chain reaction. We report here the identification and cDNA cloning of a novel GFAT1 splice variant expressed abundantly in skeletal muscle and heart. This subtype, designated GFAT1-L, contains a 54-bp insertion within the GFAT1 coding sequence. Recombinant GFAT1-L protein possessed functional GFAT activities and biochemical characteristics similar to GFAT1. Previously, GFAT1 was considered a simplex enzyme. The identification of a novel GFAT1 subtype possessing functional enzymatic activity and tissue-specific expression should provide additional insight into the mechanism of skeletal muscle insulin resistance and diabetes complications.


Subject(s)
Alternative Splicing , Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing)/biosynthesis , Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing)/chemistry , Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing)/genetics , Muscle, Skeletal/enzymology , Base Sequence , Cloning, Molecular , DNA, Complementary/metabolism , Escherichia coli/metabolism , Humans , Kinetics , Molecular Sequence Data , Polymerase Chain Reaction , Protein Isoforms , RNA, Messenger/metabolism , Reverse Transcriptase Polymerase Chain Reaction , Sequence Homology, Nucleic Acid , Tissue Distribution
4.
J Clin Invest ; 108(3): 371-81, 2001 Aug.
Article in English | MEDLINE | ID: mdl-11489930

ABSTRACT

Insulin stimulates glucose uptake by recruiting glucose transporter 4 (GLUT4) from an intracellular compartment to the cell surface; this phenomenon is defective in type 2 diabetes. Here we examine the involvement of actin filaments in GLUT4 translocation and their possible defects in insulin resistance, using L6 myotubes expressing myc-tagged GLUT4. Insulin caused membrane ruffling, a dynamic distortion of the myotube dorsal surface. Fluorescence microscopy and immunogold staining of surface GLUT4myc coupled to backscatter electron microscopy revealed a high density of this protein in membrane ruffles. The t-SNAREs syntaxin4 and SNAP-23 were also abundant in these regions. Below the membrane, GLUT4 and the vesicular protein VAMP2, but not VAMP3, colocalized with the actin structures supporting the membrane ruffles. GLUT4myc externalization and membrane ruffles were reduced by jasplakinolide and by swinholide-A, drugs that affect actin filament stability and prevent actin branching, respectively. Insulin resistance generated by prolonged (24 hours) exposure of myotubes to high glucose and insulin diminished the acute insulin-dependent remodeling of cortical actin and GLUT4myc translocation, reminiscent of the effect of swinholide-A. We propose that GLUT4 vesicle incorporation into the plasma membrane involves insulin-dependent cortical actin remodeling and that defective actin remodeling contributes to insulin resistance.


Subject(s)
Actins/metabolism , Insulin/pharmacology , Monosaccharide Transport Proteins/metabolism , Muscle Proteins , Muscle, Skeletal/drug effects , Muscle, Skeletal/metabolism , Actins/ultrastructure , Animals , Biological Transport, Active/drug effects , Carrier Proteins/metabolism , Cell Line , Cell Membrane/drug effects , Cell Membrane/metabolism , Cell Membrane/ultrastructure , Glucose/pharmacology , Glucose Transporter Type 4 , Insulin Resistance , Membrane Proteins/metabolism , Microscopy, Electron, Scanning , Microscopy, Fluorescence , Muscle, Skeletal/ultrastructure , Qa-SNARE Proteins , Qb-SNARE Proteins , Qc-SNARE Proteins , R-SNARE Proteins , Rats , Vesicle-Associated Membrane Protein 3
5.
Biol Signals Recept ; 9(5): 267-74, 2000.
Article in English | MEDLINE | ID: mdl-10965062

ABSTRACT

We investigated a nonradioisotope method for the evaluation of glucose uptake activity using enzymatic measurement of 2-deoxyglucose 6-phosphate (2DG6P) content in isolated rat soleus muscle in vitro and in vivo. The 2DG6P content in isolated rat soleus muscle after incubation with 2-deoxyglucose (2DG) was increased in a dose-dependent manner by insulin (ED(50) = 0.6 mU/ml), the maximum response being about 5 times that of the basal content in vitro. This increment was completely abolished by wortmannin (100 nM), with no effect on basal 2DG6P content. An insulin-mimetic compound, vanadium, also increased 2DG6P content in a dose-dependent manner. In isolated soleus muscle of Zucker fa/fa rats, well known as an insulin-resistant model, insulin did not increase 2DG6P content. The 2DG6P content in rat soleus muscle increased after 2DG (3 mmol/kg) injection in vivo, and conversely, the 2DG concentration in plasma was decreased in a dose-dependent manner by insulin (ED(50) = 0.11 U/kg). The maximum response of the accumulation of 2DG6P in soleus muscle was about 4 times that of the basal content. This method could be useful for evaluating glucose uptake (transport plus phosphorylation) activity in soleus muscle in vitro and in vivo without using radioactive materials.


Subject(s)
Glucose-6-Phosphate/pharmacokinetics , Glucose/metabolism , Muscle, Skeletal/metabolism , Androstadienes/pharmacology , Animals , Deoxyglucose/blood , Dose-Response Relationship, Drug , Enzyme Inhibitors/pharmacology , Female , Glucose-6-Phosphate/analogs & derivatives , In Vitro Techniques , Insulin/pharmacology , Male , Rats , Rats, Sprague-Dawley , Rats, Zucker , Vanadium/pharmacology , Wortmannin
6.
Am J Physiol ; 277(3): E572-8, 1999 09.
Article in English | MEDLINE | ID: mdl-10484371

ABSTRACT

Insulin stimulates glucose uptake into muscle and fat cells via recruitment of the glucose transporter 4 (GLUT-4) from intracellular store(s) to the cell surface. Robust stimulation of glucose uptake by insulin coincides with the expression of GLUT-4 during differentiation of muscle and fat cells, but it is not known if GLUT-4 expression suffices to confer insulin sensitivity to glucose uptake. We have therefore examined the effect of expression of a myc epitope-tagged GLUT-4 (GLUT-4myc) into L6 myoblasts, which do not express endogenous GLUT-4 until differentiated into myotubes. Ectopic expression of GLUT-4myc markedly improved insulin sensitivity of glucose uptake in L6 myoblasts. The GLUT-4myc protein distributed equally to the cell surface and intracellular compartments in myoblasts, and the intracellular fraction of GLUT-4myc further increased in myotubes. In myoblasts, the intracellular GLUT-4myc compartment contained the majority of the insulin-regulatable amino peptidase (IRAP) but less than half of the GLUT-1, suggesting segregation of GLUT-4myc and IRAP to a specific cellular locus. Insulin stimulation of phosphatidylinositol 3-kinase and protein kinase B-alpha activities was similar for L6-GLUT-4myc myoblasts and myotubes. At both stages, GLUT-4myc responded to insulin by translocating to the cell surface. These results suggest that GLUT-4myc segregates into a specific compartment in L6 myoblasts and confers insulin sensitivity to these cells. L6-GLUT-4myc myoblasts, which are easily transfectable with various constructs, are a useful resource to study insulin action.


Subject(s)
Fibroblasts/metabolism , Insulin/physiology , Monosaccharide Transport Proteins/metabolism , Muscle Proteins , Muscle, Smooth/metabolism , Proto-Oncogene Proteins , Aminopeptidases/metabolism , Biological Transport , Cell Differentiation/physiology , Cell Line , Cystinyl Aminopeptidase , Fibroblasts/cytology , Genes, myc , Glucose Transporter Type 1 , Glucose Transporter Type 4 , Monosaccharide Transport Proteins/genetics , Muscle, Smooth/cytology , Phosphatidylinositol 3-Kinases/metabolism , Protein Serine-Threonine Kinases/metabolism , Proto-Oncogene Proteins c-akt , Sequence Tagged Sites , Tissue Distribution
7.
J Biol Chem ; 274(15): 10071-8, 1999 Apr 09.
Article in English | MEDLINE | ID: mdl-10187787

ABSTRACT

The precise mechanisms underlying insulin-stimulated glucose transport still require investigation. Here we assessed the effect of SB203580, an inhibitor of the p38 MAP kinase family, on insulin-stimulated glucose transport in 3T3-L1 adipocytes and L6 myotubes. We found that SB203580, but not its inactive analogue (SB202474), prevented insulin-stimulated glucose transport in both cell types with an IC50 similar to that for inhibition of p38 MAP kinase (0.6 microM). Basal glucose uptake was not affected. Moreover, SB203580 added only during the transport assay did not inhibit basal or insulin-stimulated transport. SB203580 did not inhibit insulin-stimulated translocation of the glucose transporters GLUT1 or GLUT4 in 3T3-L1 adipocytes as assessed by immunoblotting of subcellular fractions or by immunofluorescence of membrane lawns. L6 muscle cells expressing GLUT4 tagged on an extracellular domain with a Myc epitope (GLUT4myc) were used to assess the functional insertion of GLUT4 into the plasma membrane. SB203580 did not affect the insulin-induced gain in GLUT4myc exposure at the cell surface but largely reduced the stimulation of glucose uptake. SB203580 had no effect on insulin-dependent insulin receptor substrate-1 phosphorylation, association of the p85 subunit of phosphatidylinositol 3-kinase with insulin receptor substrate-1, nor on phosphatidylinositol 3-kinase, Akt1, Akt2, or Akt3 activities in 3T3-L1 adipocytes. In conclusion, in the presence of SB203580, insulin caused normal translocation and cell surface membrane insertion of glucose transporters without stimulating glucose transport. We propose that insulin stimulates two independent signals contributing to stimulation of glucose transport: phosphatidylinositol 3-kinase leads to glucose transporter translocation and a pathway involving p38 MAP kinase leads to activation of the recruited glucose transporter at the membrane.


Subject(s)
Adipocytes/metabolism , Calcium-Calmodulin-Dependent Protein Kinases/antagonists & inhibitors , Enzyme Inhibitors/pharmacology , Glucose/metabolism , Imidazoles/pharmacology , Insulin/pharmacology , Mitogen-Activated Protein Kinases , Monosaccharide Transport Proteins/metabolism , Muscle Proteins , Muscles/metabolism , Nerve Tissue Proteins , Proto-Oncogene Proteins , Pyridines/pharmacology , 3-O-Methylglucose/metabolism , 3T3 Cells , Adipocytes/drug effects , Animals , Biological Transport/drug effects , Deoxyglucose/metabolism , Enzyme Inhibitors/chemistry , Glucose Transporter Type 1 , Glucose Transporter Type 3 , Glucose Transporter Type 4 , Imidazoles/chemistry , Mice , Muscles/drug effects , Phosphorylation , Protein Serine-Threonine Kinases/metabolism , Proto-Oncogene Proteins c-akt , Pyridines/chemistry , p38 Mitogen-Activated Protein Kinases
8.
Am J Physiol ; 275(6): C1487-97, 1998 12.
Article in English | MEDLINE | ID: mdl-9843710

ABSTRACT

2,4-Dinitrophenol (DNP) uncouples the mitochondrial oxidative chain from ATP production, preventing oxidative metabolism. The consequent increase in energy demand is, however, contested by cells increasing glucose uptake to produce ATP via glycolysis. In L6 skeletal muscle cells, DNP rapidly doubles glucose transport, reminiscent of the effect of insulin. However, glucose transport stimulation by DNP does not require insulin receptor substrate-1 phosphorylation and is wortmannin insensitive. We report here that, unlike insulin, DNP does not activate phosphatidylinositol 3-kinase, protein kinase B/Akt, or p70 S6 kinase. However, chelation of intra- and extracellular Ca2+ with 1,2-bis(2-aminophenoxy)ethane-N,N,N', N'-tetraacetic acid-AM in conjunction with EGTA inhibited DNP-stimulated glucose uptake by 78.9 +/- 3.5%. Because Ca2+-sensitive, conventional protein kinase C (cPKC) can activate glucose transport in L6 muscle cells, we examined whether cPKC may be translocated and activated in response to DNP in L6 myotubes. Acute DNP treatment led to translocation of cPKCs to plasma membrane. cPKC immunoprecipitated from plasma membranes exhibited a twofold increase in kinase activity in response to DNP. Overnight treatment with 4-phorbol 12-myristate 13-acetate downregulated cPKC isoforms alpha, beta, and gamma and partially inhibited (45.0 +/- 3.6%) DNP- but not insulin-stimulated glucose uptake. Consistent with this, the PKC inhibitor bisindolylmaleimide I blocked PKC enzyme activity at the plasma membrane (100%) and inhibited DNP-stimulated 2-[3H]deoxyglucose uptake (61.2 +/- 2.4%) with no effect on the stimulation of glucose transport by insulin. Finally, the selective PKC-beta inhibitor LY-379196 partially inhibited DNP effects on glucose uptake (66.7 +/- 1.6%). The results suggest interfering with mitochondrial ATP production acts on a signal transduction pathway independent from that of insulin and partly mediated by Ca2+ and cPKCs, of which PKC-beta likely plays a significant role.


Subject(s)
Calcium/physiology , Cytosol/metabolism , Glucose/metabolism , Mitochondria, Muscle/metabolism , Muscle Proteins , Protein Kinase C/physiology , Uncoupling Agents/pharmacology , 2,4-Dinitrophenol/pharmacology , 3-O-Methylglucose/pharmacokinetics , Biological Transport/drug effects , Biological Transport/physiology , Cell Line , Glucose Transporter Type 4 , Insulin/physiology , Intracellular Membranes/metabolism , Monosaccharide Transport Proteins/pharmacokinetics , Muscle, Skeletal/cytology , Muscle, Skeletal/metabolism , Signal Transduction/drug effects
9.
Cell Mol Biol ; 37(4): 475-80, 1991.
Article in English | MEDLINE | ID: mdl-1934019

ABSTRACT

Phagocytic activity of alveolar macrophages (AM) was enhanced by pulmonary surfactant obtained from bronchoalveolar lavage fluid of rats starved for 2 days, as compared to fed. The enhanced activity of phagocytosis was dependent on the dose of surfactant. The prepared surfactant showed a different protein to phospholipid ratio of 0.108 in fed and 0.234 in 2 days starved, because of an increased ratio of protein in surfactant from 2 days starved rats. F(ab')2 anti-surfactant protein inhibited the enhanced AM phagocytosis by surfactant. These results suggested that the enhancement of AM phagocytosis in 2 days starved rats was on account of an increase of protein in their surfactant compared to fed.


Subject(s)
Macrophages, Alveolar/immunology , Phagocytosis/drug effects , Pulmonary Surfactants/pharmacology , Starvation/immunology , Animals , Bronchoalveolar Lavage Fluid/chemistry , Immunoglobulin Fab Fragments/immunology , Macrophages, Alveolar/drug effects , Male , Phospholipids/analysis , Proteins/analysis , Pulmonary Surfactants/chemistry , Pulmonary Surfactants/immunology , Rats , Rats, Inbred F344
10.
Cell Mol Biol ; 37(3): 353-8, 1991.
Article in English | MEDLINE | ID: mdl-1834340

ABSTRACT

Histochemical localization of cathepsin B in alveolar macrophages (AM) that separated into four different density fractions (I, II, III and IV) by discontinuous Percoll gradient centrifugation was demonstrated in fluorescence microscope using CBZ-Arg-Arg-4-methoxy-2- naphthylamide as a substrate and 5-nitrosalicylaldehyde as a coupling reagent. The least dense AM (fraction I) was found numerous bright yellow fluorescing particles with high intensity in small granules distributed throughout the cytoplasm when compared to the most dense cells (fraction IV). The different localization of cathepsin B activity in the fractionated cells suggested differentiation of lysosomal system and existence of maturational (or aging) sequence in rat AM.


Subject(s)
Cathepsin B/analysis , Macrophages/enzymology , Animals , Male , Microscopy, Fluorescence , Pulmonary Alveoli/cytology , Rats , Rats, Inbred F344 , Specific Pathogen-Free Organisms
11.
Cell Mol Biol ; 37(1): 85-94, 1991.
Article in English | MEDLINE | ID: mdl-2059988

ABSTRACT

When coupled with separation of alveolar macrophages (AM) into four different density fractions (I, II, III and IV) by discontinuous Percoll gradient centrifugation, ultrastructural heterogeneity was evident in secreting process of lysosomal enzymes. Lower dense AM (I and II) released high levels of acid phosphatase and cathepsin B, whereas higher dense ones (III and IV) did not. Ultrastructurally, there were multiple ruffling and active extension of long cytoplasmic processes from one pole or around the cell surface of AM obtained from the higher density fractions. In contrast, AM from lower dense fractions had much less cytoplasmic processes and contained more cytoplasmic vacuoles showing positive reactions of acid phosphatase. These cells featured more frequently round or ovoid knobs with acid phosphatase activity along and from the tips of the cytoplasmic processes, suggestive of exocytosis. It was suggested that these ultrastructural changes linked to the maturation process and release of lysosomal enzymes from differentiated AM.


Subject(s)
Lysosomes/enzymology , Macrophages/enzymology , Acid Phosphatase/analysis , Animals , Cathepsin B/analysis , Cell Separation , Centrifugation, Density Gradient , Lung , Macrophages/ultrastructure , Male , Rats , Rats, Inbred F344
13.
Biorheology ; 20(5): 459-70, 1983.
Article in English | MEDLINE | ID: mdl-6677272

ABSTRACT

Specimens of bave (undegummed silk thread) were collected from cocoons of various origins of parent silkworm races, such as Japanese, Chinese, European, Korean and tropical origins, and from as many races as possible. An apparatus was set up to measure the dynamic elastic modulus of these specimens. In all the categories of the races tested, the elastic modulus was linearly related to the size of bave, regardless of the portion of cocoon layer from which the specimens were taken. This correlation was concluded to be universal to the silk thread of Bombyx mori L. species; however, values of the regression coefficient and of the elastic modulus were susceptible to the origin of silkworm races, depending on whether they were native or improved.


Subject(s)
Insect Proteins , Proteins/physiology , Animals , Bombyx , Elasticity , Regression Analysis , Silk , Species Specificity
SELECTION OF CITATIONS
SEARCH DETAIL
...