Direct measurement of the Chudakov effect.

Experimental results for the restricted energy loss of pairs created from 1-178 GeV photons in a thin Au target and subsequently passing a CCD detector are presented. It is shown that pairs--when detected close to the creation vertex--suffer a reduced energy loss due to the internal screening of the charges constituting the pair. Furthermore, the ability to measure directly the energy of the pair by calorimetry enables a comparison with theory as a function of energy. The observed phenomenon is in good qualitative agreement with general expectations from the Chudakov effect but indicates a quantitative disagreement with either of two mutually disagreeing theories.

Electromagnetically induced nuclear-charge pickup observed in ultrarelativistic Pb collisions.

A strong increase of inclusive nuclear-charge pickup cross sections, forming 83Bi from 158A GeV 82Pb ions, is observed in comparison to similar measurements at 10.6A GeV. From the dependence of these cross sections on target atomic number, this increase is attributed to the electromagnetic process of pion production by equivalent photons. The observed cross sections can be reproduced quantitatively using the recently developed RELDIS code.

First measurements of the unique influence of spin on the energy loss of ultrarelativistic electrons in strong electromagnetic fields.

Although some authors have claimed that the effect is not detectable, we show experimentally for the first time that as the quantum parameter chi grows beyond 1, an increasingly large part of the hard radiation emitted arises from the spin of the electron. Results for the energy loss of electrons in the energy range 35-243 GeV incident on a W single crystal are presented. Close to the axial direction the strong electromagnetic fields induce a radiative energy loss which is significantly enhanced compared to incidence on an amorphous target. In such continuously strong fields, the radiation process is highly nonperturbative for ultrarelativistic particles and a full quantum description is needed. The remarkable effect of spin flips and the energy loss is connected to the presence of a field comparable in magnitude to the Schwinger critical field, E0 = m(2)c(3)/ePlanck's over 2pi, in the rest frame of the emitting electron.

Significance of plasmalemma disruption in bovine and equine spermatozoa.

We have investigated fresh and cryopreserved bovine and equine spermatozoa using light and transmission soft X-ray microscopy. Spermatozoa were examined, in the presence or absence of semen, after using Percoll gradient centrifugation and re-suspending in medium. X-ray microscopy provided high resolution (30 nm) transmission images of whole cells in solution with high contrast, while retaining the simple preparation techniques used in light microscopy. We demonstrated translucent, membrane-bound vesicles in the acrosomal and midpiece regions that were similar in size and we noted their incidence in both fresh and frozen-thawed material from both animals. The vesicles were formed by the separation and expansion of the plasmalemma away from the underlying structure but were not caused by the freeze-thaw process. We suggest that these structures form part of the normal ultrastructure of spermatozoa and are damaged during preparation of the samples for transmission electron microscopy, resulting in a structure previously and incorrectly identified as damaged by the freezing and thawing process.

X-ray microscopy of human spermatozoa shows change of mitochondrial morphology after capacitation.

Using X-ray microscopy two morphologically distinct states were observed of the human spermatozoan mitochondria: (i) compact and tightly wrapped around the axoneme, and (ii) morphologically transformed, i.e. with circular areas of high X-ray transmission, either loosely wrapped around the axoneme or distended. The spermatozoa were examined at two stages of their post-ejaculation maturation process, i.e. as present in fresh ejaculated semen and after in-vitro capacitation. X-ray microscopy allowed sample preparation that was as simple as for conventional light microscopy whilst giving high resolution (30 nm) imaging of samples in liquid media compatible with the requirements of live biological specimens. The specimens were not fixed, stained or metal coated. These features make X-ray microscopy useful in the study of cells, particularly cells in suspension. The relative frequencies of the two morphological states of the mitochondria in seminal plasma and after in-vitro capacitation were compared. In seminal plasma, almost all spermatozoa had compact and tightly wrapped mitochondria. After harvesting by swim-up technique, an increase in the morphologically transformed state had occurred. However, the greatest increase in the morphologically transformed state occurred when the sample had been incubated under capacitating conditions. In this case almost all spermatozoa had morphologically transformed mitochondria.