ABSTRACT
An immunosensor is based on the signal measurement obtained upon the reaction of an antibody antigen complex. It plays a significant role in various fields such as environmental analysis, production monitoring, drug detection or screening, veterinary medicine, and animal food. In this study, an antibody crosslinked graphen oxide (GO)-based potentiometric sensor has been developed for fast, simple, and economical detection of clenbuterol. In this context, the photosensitive amino acid bound GO platform is synthesized and used for the preparation of electrode material. Then, polymeric structure is characterized by infrared spectroscopy, and the performance of immunonano platform prepared by potentiometric sensor is evaluated. The effect of pH, response time, selectivity, and sensitivity is investigated. Under the optimized conditions, a simple and rapid method for the determination of clenbuterol from milk sample is established by immuno-potentiometric sensor. The detection limit has found to be 0.87 × 10-9 mmol L-1 for this immuno-potentiometric sensor. This immuno-potentiometric sensor has optimum pH at 7.0, a wide linear response (1.0 × 10-2 to 1.0 × 10-9 mmol L-1 ), rapid response time (2 Min) and 36 weeks operational lifetime.
Subject(s)
Antibodies/chemistry , Clenbuterol/analysis , Cross-Linking Reagents/chemistry , Graphite/chemistry , Immunoassay , PotentiometryABSTRACT
In this study, a simple, rapid and sensitive method based on novel molecular imprinted polymeric sensor has been developed and validated for the determination of prostate cancer metabolite biomarker. The molecularly imprinted polymer (MIP) has been synthesized by emulsion polymerization, using sarcosine as template molecule, methacryloylamido histidine (MAH) as functional monomer and ethylene glycol dimethacrylate (EDMA) as cross-linking agent. The performance of the developed sarcosine sensor has been evaluated, and the results have indicated that a sensitive potentiometric sensor has been fabricated. The sarcosine sensor has showed high-selectivity, shorter response time (<2min), wider linear range (10(-2)-10(-6)mM), lower detection limit (1.35×10(-7)mM), and satisfactory long-term stability (>5.5months).