A case of colonic-colonic intussusception in a dog secondary to lymphoma treated with colonic resection and anastomosis.

A 2-year-old castrated male Great Dane crossbreed dog was presented with a history of diarrhea and suspected intussusception. Abdominal ultrasound revealed a colonic-colonic intussusception. The gastrointestinal tract was explored, and an approximately 5-cm intussusception was discovered mid-colon. All other gastrointestinal structures were normal in appearance. The intussusception could not be reduced manually. A colonic resection and anastomosis were performed together with a left-sided incisional colopexy. The dog recovered from surgery and histopathology revealed the intussusception to be secondary to large cell transmural lymphoma. Sections from the surgical margins revealed proliferation of fibrovascular tissue along the serosal surface segmentally, but no neoplastic cells were identified. The dog was subsequently treated with chemotherapy consisting of doxorubicin and prednisone. No evidence of disease recurrence was noted on ultrasound 9 months after surgery. Approximately 2 years after surgery, the dog is noted to be clinically normal at home with no abnormal findings on physical examination. A complete blood (cell) count and chemistry obtained at this time revealed no significant abnormalities besides mild azotemia. Additional restaging was declined by the owner.

Un cas d'intussusception colon-colon chez un chien secondaire à un lymphome traité par résection c olonique et anastomose. Un grand danois croisé mâle castré âgé de 2 ans a été présenté avec des antécédents de diarrhée et une suspicion d'intussusception. L'échographie abdominale a révélé une intussusception colon-colon. Le tractus gastro-intestinal a été exploré et une intussusception d'environ 5 cm a été découverte au milieu du côlon. Toutes les autres structures gastro-intestinales avaient un aspect normal. L'intussusception n'a pas pu être réduite manuellement. Une résection colonique et une anastomose ont été réalisées avec une colopexie incisionnelle du côté gauche. Le chien a récupéré de la chirurgie et de l'histopathologie a révélé que l'intussusception était secondaire à un lymphome transmural à grandes cellules. Des sections des marges chirurgicales ont révélé une prolifération de tissu fibrovasculaire le long de la surface séreuse de manière segmentaire, mais aucune cellule néoplasique n'a été identifiée. Le chien a ensuite été traité par une chimiothérapie composée de doxorubicine et de prednisone. Aucun signe de récidive de la maladie n'a été noté à l'échographie 9 mois après la chirurgie. Environ 2 ans après la chirurgie, le chien est cliniquement normal à la maison sans résultats anormaux à l'examen physique. Une numération sanguine (cellule) complète et l'analyse chimique obtenues à ce moment n'ont révélé aucune anomalie significative outre une légère azotémie. Une nouvelle restadification a été refusée par le propriétaire.(Traduit par Dr Serge Messier).

Correlation of distraction index with arthroscopic findings in juvenile dogs with hip dysplasia.

OBJECTIVE: To compare distraction index (DI) with hip arthroscopy findings in juvenile dogs with hip dysplasia undergoing unilateral or bilateral double pelvic osteotomy (DPO). STUDY DESIGN: Retrospective case series. ANIMALS: Twenty dogs (36 hip joints). METHODS: Medical records from dogs undergoing unilateral or bilateral DPO (2011-2016) were reviewed. Distraction index was measured by using distraction view radiographs. Arthroscopic images were analyzed. Cartilage was graded by using the modified Outerbridge scale (0 = arthroscopically normal, 1 = chondromalacia, 2 = surface fibrillation, 3 = deep fissuring, 4 = full-thickness cartilage loss, and 5 = eburnation of exposed subchondral bone). Dorsal acetabular rim, acetabular labrum, the ligament of head of the femur, and synovitis were graded by using a four-point scale (normal, mild, moderate, severe). Spearman and Pearson correlations were used to compare DI between grade groups and age. RESULTS: Distraction index ranged from 0.4 to 1.0 (mean 0.69 ± 0.16). Most of the hips had an arthroscopically normal femoral head and acetabulum in most regions, except for the region at the insertion of the ligament of the head of the femur. There was a low positive correlation between synovitis and DI (Spearman ρ = 0.35, P = .0346). No other differences in DI were identified between grade or age groups. CONCLUSION: There was a low positive correlation between synovitis and DI. No other differences in DI were identified. CLINICAL SIGNIFICANCE: Arthroscopic evaluation of the coxofemoral joint prior to DPO can allow detection of joint pathology that might not be appreciated by using traditional radiographic screening criteria.

Extracellular Vesicles: Evolutionarily Conserved Mediators of Intercellular Communication.

Extracellular vesicles (EV) are sub-micron circulating vesicles found in all bodily fluids and in all species so far tested. They have also recently been identified in seawater and it has further been shown that they are released from microorganisms and may participate in interspecies communication in the gut. EV are typically composed of a lipid bilayer formed from the plasma membrane and which encases a cargo that can include genetic material, proteins, and lipids. At least two different processes of formation and release have been described in mammalian cells. The exosome population (50 to 150nm size) are produced via a lyso-endosomal pathway, while microvesicles (100 to 1000nm) are formed by budding of the plasma membrane in a calcium dependent process. Both pathways are highly regulated and appear to be conserved amongst different species. EV release has been shown to be upregulated in a number of human chronic diseases including cardiovascular disease, metabolic disorders, obesity, and cancer; evaluation of their presence in veterinary samples may aid diagnosis in the future. This review will provide insight into the formation of EV and their detection in bodily fluids from different veterinary species and how they may provide a novel addition to the veterinary toolkit of the future.