ABSTRACT
We have used Y-specific cosmid clones in a random fingerprinting approach to build contigs on the human Y chromosome. Clones derived from two libraries have been analyzed. The construction of one library is described here, the second was the Y chromosome-specific library LLOYNCO3 "M" (Lawrence Livermore National Laboratory). To date, we have fingerprinted 4430 cosmids: 377 contigs have been constructed containing from 2 to 39 clones. Along with the singletons, we estimate that we have covered 72.5% of the euchomatic portion of the Y chromosome with fingerprinted clones. Sequence tagged sites are being used to anchor cosmids and contigs onto the YAC framework.
Subject(s)
Chromosome Mapping/methods , Cosmids , DNA Fingerprinting , Y Chromosome , Base Sequence , Cloning, Molecular , DNA Primers , Humans , Molecular Sequence DataABSTRACT
A CA repeat, subcloned from a cosmid, has been assigned to chromosome 12 by use of a panel of somatic cell hybrids. The assignment was confirmed by fluorescent in situ hybridization and the site further localized to 12q14. The repeat is highly polymorphic and should be useful for genetic mapping.
