ABSTRACT
OBJECTIVE: To evaluate the histomorphometric effects of different molecular weight hyaluronic acid on bone formation in rats after expansion of the interpremaxillary suture. MATERIAL AND METHODS: Twenty-four male Sprague Dawley rats were divided into three groups. Each group was subjected to expansion for 5 days and retention for 10 days. Group 1 received 50 µl of high molecular weight hyaluronic acid (HMWHA), group 2 received 50 µl of low molecular weight hyaluronic acid (LMWHA), and the control group received same amount of saline solution to the interpremaxillary suture. Ten days after injection, the rats were killed and their maxillas dissected. For the histomorphometric evaluation, blocks were serially sectioned at 10-µm intervals. Sections were stained with hematoxylin-eosin (HE) and evaluated with image analysis software. Bone area (µm²) (BA), bone perimeter of suture borders (µm) (BP), and ratio of osteoblast cells and capillary cells to BA and BP parameters were evaluated. RESULTS: HMWHA showed a statistically higher ratio of osteoblast and capillary cell scores compared with the LMWHA and control groups (p < 0.05). There were no statistically significant differences in between LMWHA and control groups (p > 0.05). CONCLUSIONS: Local injection of HMWHA in the interpremaxillary suture after rapid maxillary expansion stimulated new bone formation, which may shorten the retention period and may reduce the risk of relapse. LMWHA has no effect on bone formation in interpremaxillary suture.
Subject(s)
Cranial Sutures/drug effects , Cranial Sutures/growth & development , Hyaluronic Acid/pharmacology , Osteogenesis/drug effects , Palatal Expansion Technique , Animals , Capillaries/anatomy & histology , Capillaries/drug effects , Capillaries/growth & development , Cranial Sutures/anatomy & histology , Endothelial Cells/drug effects , Hyaluronic Acid/therapeutic use , Male , Maxilla/drug effects , Osteoblasts/drug effects , Random Allocation , Rats , Rats, Sprague-DawleyABSTRACT
For Class III adult patients, combined treatment strategy must be followed which includes either further dentoalveolar compensation or orthognathic surgery following decompensation of the teeth. This case report presents the interdisciplinary approach of a skeletal Class III malocclusion with increased vertical facial dimension, occlusal cant, extracted posterior teeth on the right upper and left lower segments, extensive restorations, and total circular crossbite. The orthodontic alignment took 10 months. LeFort 1 osteotomy with 7 mm advancement, 5 mm impaction on the left side, 3 mm impaction on the right side and 2 mm rotation for midline correction toward the left side, 3 mm set-back of mandible with bilateral sagittal split osteotomy were done. The surgery simulation, postoperative and 2-year follow-up records were compatible. The treatment was finalized in a straight profile with stable occlusion and good smile characteristics without airway disturbance.
