Associations between dietary intake and physical activity, as behavioral indicators, with body fat in adolescents from Mexico.

Introduction: Introduction: the prevention of cardiometabolic diseases is currently a priority due to its relationship with COVID-19 complications. Unhealthy lifestyles, like inadequate diet and physical inactivity, are the cornerstone for obesity and cardiometabolic risk. Objective: to examine the association between diet and physical activity with body fat in Mexican adolescents. Methods: the study was cross-sectional. Data included socio-demographic variables, health history and smoking habit obtained through questionnaires; blood pressure and anthropometry measurements; food and nutrient intake through 24-hour recalls; and physical activities through the International Physical Activity Questionnaire (IPAQ). Univariate analyses were used and multiple models were built by stepwise forward selection (p ≤ 0.05 and biological plausibility). Results: participants were n = 230 students, 18.5 ± 0.4 years and 54.8 % men. Three models associated with body fat were obtained. In the first model, saturated fatty acids (ß = 0.30, p = 0.028) were significant positive associated, while vigorous physical activity was significant negative associated as a protective factor (ß = -0.007, p = 0.023). In the second model, total fat (ß = 0.17, p = 0.005) was significant positive associated, and vigorous physical activity was significant negative associated (ß = -0.007, p = 0.023). The third model included fruits and vegetables (ß = -5.49, p = 0.092) and vigorous physical activity (ß = -0.006, p = 0.058) as protective factors. Conclusions: dietary intake of total fat and saturated fatty acids, and vigorous physical activity, were significantly associated with body fat, while fruits and vegetables trended toward significance, in Mexican adolescents. Community-based programs that promote intake of protective foods and reduction of risky foods, and encourage vigorous physical activity, are needed in adolescents.

Introducción: Introducción: la prevención de enfermedades cardiometabólicas es actualmente una prioridad por su relación con las complicaciones de la COVID-19. Los estilos de vida poco saludables son la piedra angular de la obesidad y el riesgo cardiometabólico. Objetivo: examinar la asociación entre dieta y actividad física con la grasa corporal en adolescentes mexicanos. Métodos: el estudio fue transversal. Los datos incluyeron variables sociodemográficas; mediciones de presión arterial y antropometría; ingesta de alimentos y nutrimentos con recordatorios de 24 horas; y actividades físicas con el cuestionario internacional de actividad física (IPAQ). Se utilizo análisis univariado y los modelos múltiples se construyeron mediante selección paso a paso hacia adelante (p ≤ 0.05 y plausibilidad biológica). Resultados: participaron n = 230 estudiantes, 18,5 ± 0,4 años y 54,8 % hombres. Se obtuvieron tres modelos asociados a la grasa corporal. En el primero, los ácidos grasos saturados (ß = 0,30, p = 0,028) se asociaron significativamente de forma positiva, y la actividad física vigorosa significativamente de forma negativa como factor protector (ß = -0,007, p = 0,023). En el segundo, la grasa total (ß = 0,17, p = 0,005) se asoció positivamente y la actividad física vigorosa, negativamente (ß = -0,007, p = 0,023). El tercer modelo incluyó frutas y verduras (ß = -5,49, p = 0,092) y actividad física vigorosa (ß = -0,006, p = 0,058) como factores protectores. Conclusiones: la ingesta dietética de grasas totales y saturadas y la actividad física vigorosa se asociaron significativamente con la grasa corporal, mientras que las frutas y verduras tendieron a ser significativas. Se necesitan programas comunitarios que promuevan la ingesta de alimentos protectores y la reducción de alimentos de riesgo, y fomenten la actividad física vigorosa en adolescentes.

Low prevalence of ideal levels in cardiovascular behavior metrics among Mexican adolescents.

BACKGROUND: Lifestyle changes when transitioning from high-school to college expose students to unhealthy behaviors associated with high cardiovascular risk. The study aimed to assess the cardiovascular behavior metrics according to the AHA criteria, in freshman college adolescents from Northwest Mexico. METHODS: The study was cross-sectional. Demographics and health history were collected by questionnaires. Four behaviors were evaluated: diet quality using a duplicated FFQ, physical activity (PA) using the IPAQ, smoking, and body mass index (BMI) percentile; blood pressure was measured as a biological metric. Intakes were averaged and summed for each food group; sodium and saturated fat were calculated using the Mexican System of Food Equivalents or the USDA Database. Metrics were categorized into ideal, intermediate or poor level according to the AHA criteria. Diet outliers (± 3 SD) were trimmed and data was tested for normality. Mean±SD were calculated for continuous and percentages for categorical variables. Chi-square test compared the prevalence of demographic variables and levels of each cardiovascular metric by sex. Independent T-test evaluated differences in anthropometrics, dietary, and PA by sex, and the prevalence of ideal vs. non-ideal dietary intakes. RESULTS: Participants were n = 228, 55.6% men, age = 18.5±0.4 y. A higher prevalence of men indicated working, playing sports, and family history hypertriglyceridemia (p < 0.05). Men showed higher weight, height, BMI, waist, blood pressure, and lower PA and body fat (p < 0.05). Concerning diet quality, significant differences by sex were observed in nuts and seeds (1.1±0.6 and 0.9±0.6 oz/week, p = 0.042) and processed meats (749.8±639 and 503.6±300.3 g/week, p = 0.002); only fish and shellfish group reached AHA recommendations (513.1 ± 450.7 vs. 501.7 ± 428 g/week, p = 0.671) for men and women, respectively. Ideal level was reached by 70.9% participants for BMI percentile, 87% for smoking, 67.2% for blood pressure, 25.9% for PA, and 12.2% for diet score. Regarding food groups and nutrients, the lower prevalence in the ideal level was for sugar-sweetened beverages (10%, p = 0.013) and processed meats (4.8%, p = 0.208), and the highest for fish and shellfish (87.8%, p = 0.281) . CONCLUSIONS: The diet and PA patterns of Northwest Mexican freshman adolescents make them a high-risk group for developing long-term unhealthy habits and cardiovascular complications early in adulthood.

Reproducibility and validity of a food frequency questionnaire to assess cardiovascular health-related food intake among Mexican adolescents.

There is a lack of region-adapted tools to evaluate diet as a risk factor for cardiovascular disease (CVD) in adolescents. The study aim was to evaluate the reproducibility and validity of a paper-based and region-adapted food frequency questionnaire (FFQ) designed to assess CVD-related food and nutrient intakes of adolescents from Northwest México. The study design was cross-sectional. The FFQ was developed in a two-step process: prototype designing and a pilot test, with re-tested in a 3-month period, along with two administrations of 24 h-recall (24 hR). Pearson's and intra-class correlation coefficients (PCC and ICC) were assessed. Bland-Altman plots, limits of agreement and quintile classifications were carried out. Participants (n 221) were 53·8 % male, 18·5 ± 0·4 years old. Reproducibility had a median PCC = 0·66 for processed meats, ranging from 0·40 (saturated fat) to 0·74 (fish & shellfish), P = 0·001. ICC ranged from 0·53 (saturated fat) to 0·80 (sodium; and nuts, seeds and legumes), P = 0·001. Validity comparing FFQ1 v. 24 hR mean, PCCs ranged from 0·12 (P = 0·06) to 0·95 (P = 0·001), and ICC from 0·20 (P = 0·048) to 0·88 (P = 0·001); comparing FFQ2 v. 24 hR mean, PCCs ranged from 0·07 (P = 0·25) to 0·46 (P = 0·001), and ICC from 0·15 (P = 0·106) to 0·58 (P = 0·001). The FFQ overestimated the intake of all food groups and nutrients (P < 0·05), while Cohen's κ showed coefficients lower than 0·20. The proposed FFQ represents a moderately validated tool to estimate CVD-related food and nutrient intakes as a risk factor, which can be used in combination with multiple administrations of 24 hRs, as a critical mean in future interventions intended to reduce cardiometabolic risk in adolescents.

Simultaneous extraction of antibiotic and estrogen from animal blood serum using aqueous two-phase systems as predictor of environmental impact.

Efficient analytical methods are required for optimizing dosage of veterinary antibiotics and hormones in order to reduce toxicity and antimicrobial resistance in the environment. Thus, the objective of this work was to develop a rapid and low-cost method for determination of hormone estradiol and antibiotic chlortetracycline in bovine and porcine blood serum by aqueous two-phase system (ATPS) extraction and capillary electrophoresis quantification. ATPS based on ionic liquid cholinium alaninate and citrate salt along with mixtures of protic and aprotic polar solvents were evaluated in terms of recovery of extraction (%R). The liquid-liquid equilibrium, phase diagrams, and tie lines are discussed. Antibiotic migrated to solvent-rich phase (R ≈ 89.0%) to all systems. Estradiol migrates to ionic liquid-rich phase; however, addition of 10% methanol changed partition to solvent-rich phase (R ≈ 89.7%). The method has high recovery and cleanliness, is cost-efficient, scalable, and hence is adequate for screening of antibiotics and hormones tested in animal blood serum for dosage optimization and to predict their environment.

Simultaneous quantification of antibiotics in wastewater from pig farms by capillary electrophoresis.

Pig farming is an important activity in the economic development of Mexico with millions of tons of meat produced annually. Antibiotics are used in therapeutic dose to prevent diseases, and sometimes as growth promoters. These compounds are not completely metabolized; they are carried into the environment in its active form at concentrations that could induce antibiotic resistance in bacteria, which could be transferred to human pathogens by horizontal gene transfer. The objective of this work was to develop methods of analysis for simultaneous quantification of the antibiotics Oxytetracycline (OXT), Chlortetracycline (CLT), Enrofloxacin (ENRO) and Ciprofloxacin (CIPRO) by field-amplified sampling injection in capillary zone electrophoresis (FASI-CZE). The method was validated by parameters of (1) linearity, obtaining a lineal range of 0.05 at 1 µg mL-1 for ENRO and CIPRO, and from 0.1 to 1 µg mL-1 for OXT and CLT; (2) precision, obtaining values <5% of standard deviation for CIPRO and ENRO and <10% of standard deviation for OXT and CLT; (3) accuracy, with recovery values from 93 to 115%; (4) selectivity, with values of resolution >2 for the all antibiotics tested. To prove the method, a sample of wastewater from a local pig farm was analyzed, detecting a concentration of 0.140 ±â€¯0.009 for OXT. This concentration was higher than the minimal selective concentration, indicating the point in which resistance to a determined antibiotic could develop. The methods were validated with precision and sensitivity comparable to chromatographic methods, which can be used to analyze wastewater from pig farms directly.