The development and use of an inactivated vaccine against animals trichophytosis.

Background: The annual increase in the number of camels entails a parallel increase in the incidence of trichophytosis, which poses a great threat to the health and life of both this species of animals and other organisms that contact and surround them. Aim: The aim of the study was to develop and establish the quality of vaccines inactivated by ultrasonic exposure for the prevention and treatment of trichophytosis in camels, and to compare them with chemically deactivated vaccines. Methods: The peculiarity of the technology of production of these vaccines was the use of an innovative method of inactivation of fungal strains by ultrasonic waves, which allowed to achieve high positive results in theory, and was subsequently confirmed in practice by immunizing sick and healthy animals. The first tests of the obtained vaccines were conducted in laboratory conditions on experimental rabbits. Results: The results of prophylactic and therapeutic vaccinations were one hundred percent positive, which made it possible to conduct further tests directly on camels of industrial farms, the expected result of which was also positively confirmed at the end of the research. Conclusion: As a result of this experiment, the effectiveness, stability, and safety of the manufactured vaccines were established, which made it possible to approve the regulatory and technical documentation and patent them as an innovative and effective development for the prevention and treatment of camel trichophytosis, which will reduce the growth of infection and further overcome the mass spread of the disease both among camels and among the surrounding organisms to which it is transmitted.

Comparative assessment of regulated methods and PCR in the diagnosis of trichophytosis in veterinary mycology.

Background: There is an increase in the incidence of human and animal infectious skin diseases of fungal etiology in the world. The main source of infecting the population has become agricultural and stray animals. Aim: The objective of this study was to examine the morphophysiological and microbiological characteristics of pathogenic fungi belonging to the species Trichophyton verrucosum. This species is known to cause diseases in both humans and livestock in Kazakhstan. In addition, the study aimed to assess the feasibility of using the polymerase chain reaction (PCR) method for detecting T. verrucosum. This assessment was conducted in comparison to the outcomes of conventional laboratory diagnostic tests commonly employed for trichophytosis. Methods: The research focused on analyzing 141 samples of pathological material obtained from calves in Almaty, Turkestan, and Kyzylorda regions. These calves exhibited clinical symptoms of skin disease. The study aimed to identify the causative agent using various techniques, including microscopic examination, microbiological methods involving the isolation of pure cultures, and PCR. Results: The detection of the causative agent of dermatophytosis using conventional methods was relatively low, 86% for the microscopic method, and 79% for the microbiological method with the isolation of the culture of the pathogen. Extraction and detection of the genetic material of the causative agent of the disease for PCR was carried out according to the method developed by the authors. The effectiveness of the PCR method was 97.9%, which is significantly higher (p < 0.05) compared with the diagnostic effectiveness of conventional methods. The PCR method using specific primers identified the causative agent in 98% of cases, which significantly (p < 0.05) exceeded the results obtained using conventional diagnostic methods. Accordingly, the PCR method had better sensitivity and specificity indicators. Conclusion: The conducted study recommends the method of PCR diagnosis of dermatophytosis for fast and reliable confirmation of the diagnosis of dermatophytosis in humans and animals in Kazakhstan.

Approaches to reducing the toxic exposure hazard on the sheep population.

The sheep breeding industry forms a substantial proportion of the agriculture sector in the Republic of Kazakhstan and ensuring its sustainable functioning should be implemented in a number of national documents, which can be considered as the basis for increasing the level of national food security. This can be achieved not only by increasing livestock numbers, but also by conducting research that will reduce the incidence of disease in livestock. The novelty of the study is determined by the fact that in the extension study of autopsy of sheep affected by diseases, an analysis of factors that increase the incidence of melaphagosis was carried out. Almaty and Jambyl regions were selected as objects of research. The main effects of classical and innovative drugs that reduce the melophagosis incidence are presented. The analysis of the seasonal level of morbidity and the age composition of sheep herds was carried out. It has been determined that the treatment and prevention of melophagosis can be performed on the basis of a combination of 2 and 5 drugs, both chemically active and biogenic. The practical significance of the study suggests the possibility of a considerable reduction in the level of parasitic invasions in the steppe conditions of Kazakhstan and stabilisation of the produced level of sheep breeding.

Examination of organs and tissues of adult sheep grazed in an area with possible intoxication with rocket fue, Kazakhstan

The authors have conducted experiments to study the pathoanatomical and histological pattern of organs and tissues of adult sheep affected by unsymmetric dimethylhydrazine (UDMH). This highly toxic fuel was spilled on the territory of the Karsakpay and Ulytau districts of Karaganda region, Kazakhstan, because of the fall of the rocket 'Proton­M' after an unsuccessful launch from the Baikonur cosmodrome in 2007. In the experiment, the study group was consisted of 7 adult sheep that grazed in the area of possible intoxication with rocket fuel UDMH. The main objects of the study were histological preparations obtained from fixed structures. As the structures have a flat contrast and are poorly detected in the ordinary light microscope, the specially processed preparations were used. After preparing, the authors studied organs and tissues using a microscope, which allowed to reveal in detail the level of damage caused by intoxication and to establish the negative effect of UDMH on the internal organs. The group of sheep showed a high index of macroscopic signs of interstitial pneumonia (85.7 ± 14.3%), and histologically quite high index was granulomatous inflammation of liver (71.4 ± 18.4%). Kidneys also showed a high level of abnormalities.