ABSTRACT
The aim of this study was to assess the phenotypic and genotypic diversity of 56 Arcanobacterium haemolyticum isolates isolated from 51 patients attending primary health care centres and emergency units in the health area of Santander (Cantabria, northern Spain). Phenotypic characterization was based on morphological, biochemical, and antigenic tests. Species identification was confirmed by amplification and sequencing of the 16S rDNA gene. Antimicrobial susceptibility testing was determined by microdilution following the Clinical and Laboratory Standards Institute recommendations for coryneform bacteria. Genetic diversity was evaluated using BOX-PCR and pulsed-field gel electrophoresis. Eighty percent of the isolates had an identical BOX-PCR pattern, suggesting the spread of a single clone. The present report provides extensive information on the phenotypic and genotypic characterization of A. haemolyticum.
Subject(s)
Actinomycetales Infections/microbiology , Arcanobacterium/genetics , Arcanobacterium/isolation & purification , Adolescent , Adult , Anti-Bacterial Agents/pharmacology , Arcanobacterium/cytology , Arcanobacterium/physiology , Bacterial Typing Techniques , Child , Child, Preschool , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , Electrophoresis, Gel, Pulsed-Field , Female , Humans , Male , Microbial Sensitivity Tests , Molecular Typing , RNA, Ribosomal, 16S/genetics , Sequence Analysis, DNA , Spain , Young AdultABSTRACT
Objetivo: Aplicar un método molecular, basado en la secuenciación, para la identificación de hongos de interés clínico, a nivel de especie. Método: Se estudiaron de forma prospectiva 36 cepas de hongos que con métodos convencionales no se pudieron identificar a nivel de especie y 39 muestras clínicas obtenidas por métodos invasivos, los resultados se compararon con los del cultivo. Resultados: Los métodos moleculares permitieron una identificación rápida y fiable de los organismos a nivel de especie, tanto a partir de cultivos como de muestras clínicas. Conclusión: La identificación a nivel de especie y la reducción del tiempo de respuesta, así como el valor predictivo negativo son claras ventajas de estos métodos (AU)
Objective: To apply a sequencing-based molecular method to identify clinically relevant fungi to species level. Method: Thirty-six fungi not identifiable at a species level by a conventional approach and 39 invasive clinical samples were prospectively evaluated. The results were compared with those obtained by conventional methods. Results: Molecular methods allowed rapid and reliable identification of fungi at species level, including both from organisms grown in culture and those in clinical samples. Conclusion: Molecular methods show clear advantages for fungal identification, including rapid identification at species level and high negative predictive value (AU)
Subject(s)
Humans , Fungi/isolation & purification , Mycoses/epidemiology , Molecular Diagnostic Techniques/methods , Fungi/pathogenicity , Polymerase Chain Reaction , Prospective StudiesABSTRACT
OBJECTIVE: To apply a sequencing-based molecular method to identify clinically relevant fungi to species level. METHOD: Thirty-six fungi not identifiable at a species level by a conventional approach and 39 invasive clinical samples were prospectively evaluated. The results were compared with those obtained by conventional methods. RESULTS: Molecular methods allowed rapid and reliable identification of fungi at species level, including both from organisms grown in culture and those in clinical samples. CONCLUSION: Molecular methods show clear advantages for fungal identification, including rapid identification at species level and high negative predictive value.
Subject(s)
DNA, Fungal/genetics , DNA, Ribosomal Spacer/genetics , Fungi/isolation & purification , Molecular Diagnostic Techniques , Mycology/methods , Mycoses/microbiology , Ribotyping/methods , Fungal Proteins/genetics , Fungi/classification , Fungi/genetics , Fungi/growth & development , Humans , Peptide Elongation Factor 1/genetics , Polymerase Chain Reaction/methods , Predictive Value of Tests , Prospective Studies , Sequence Analysis, DNA , Sequence Homology, Nucleic Acid , Species Specificity , Time Factors , Tubulin/geneticsSubject(s)
Esophagitis/virology , Herpes Simplex , Meat-Packing Industry , Adult , Esophagitis/diagnosis , Female , Herpes Simplex/diagnosis , HumansABSTRACT
No disponible
Subject(s)
Humans , Female , Adult , Esophagitis/virology , Herpes Simplex/complications , Simplexvirus/isolation & purification , Simplexvirus/pathogenicity , Acyclovir/therapeutic useABSTRACT
Detection of Arcanobacterium haemolyticum is based upon typical beta-hemolysis and colony morphology, but it may go undetected if only conventional sheep blood agar media for detection of beta-hemolytic streptococci are used. The influence of different culture media, atmospheres, and times of incubation for the recognition of colonies of 47 isolates of A. haemolyticum was studied. After 48 h of incubation, trypticase soy agar with 5% horse blood in 5% CO(2) was the best medium.
