Outbreaks of sarcoptic mange in free-ranging koala populations in Victoria and South Australia: a case series.

OBJECTIVE: To describe outbreaks of sarcoptic mange caused by Sarcoptes scabiei in free-ranging koalas in Victoria (December 2008 to November 2015) and South Australia (October 2011 to September 2014). METHODS: Koalas affected by mange-like lesions were reported by wildlife carers, veterinary practitioners or State Government personnel to the Faculty of Veterinary and Agricultural Sciences at The University of Melbourne and the School of Animal and Veterinary Sciences at The University of Adelaide. Skin scrapings were taken from live and dead koalas and S. scabiei mites were identified. Tissues from necropsied koalas were examined histologically. RESULTS: Outbreaks of sarcoptic mange were found to occur in koalas from both Victoria (n = 29) and South Australia (n = 29) for the first time. The gross pathological and histopathological changes are described. CONCLUSION: We present the first reported cases of sarcoptic mange outbreaks in free-ranging koalas.

Water policy effectiveness: 30 years of change in the hypernutrified Colne estuary, England.

Wastewater discharges and agricultural run-off have caused nutrient enrichment leading to eutrophication, in receiving waters worldwide. Analysis of a 30 year data set (1981-2010) for the Colne estuary, a hypernutrified estuary in the south-east of England, revealed significant reductions in nutrient concentrations in freshwater inputs and along the estuarine gradient linked to management actions. DIN concentrations decreased, mainly as a result of reduced ammonia outputs from Colchester STW and reduced nitrate loads from the catchment. Declines in phosphate concentrations occurred due to improved STW processes. There were significant declines in phytoplankton chlorophyll a over the period. Long-term trajectories of nutrient decreases were also strongly influenced by interannual patterns of rainfall and climatic signals (winter NAO). Standardised winter DIN concentrations in the Colne estuary significantly exceed the Water Framework Directive good status target, but the estuary shows no symptoms of eutrophication.

Assessing visual fields for driving in patients with paracentral scotomata.

BACKGROUND: The binocular Esterman visual field test (EVFT) is the current visual field test for driving in the UK. Merging of monocular field tests (Integrated Visual Field, IVF) has been proposed as an alternative for glaucoma patients. AIMS: To examine the level of agreement between the EVFT and IVF for patients with binocular paracentral scotomata, caused by either ophthalmological or neurological conditions, and to compare outcomes with useful field of view (UFOV) performance, a test of visual attention thought to be important in driving. METHODS: 60 patients with binocular paracentral scotomata but normal visual acuity (VA) were recruited prospectively. Subjects completed and were classified as "pass" or "fail" for the EVFT, IVF and UFOV. RESULTS: Good agreement occurred between the EVFT and IVF in classifying subjects as "pass" or "fail" (kappa = 0.84). Classifications disagreed for four subjects with paracentral scotomata of neurological origin (three "passed" IVF yet "failed" EVFT). Mean UFOV scores did not differ between those who "passed" and those who "failed" both visual field tests (p = 0.11). Agreement between the visual field tests and UFOV was limited (EVFT kappa = 0.22, IVF kappa 0.32). CONCLUSIONS: Although the IVF and EVFT agree well in classifying visual fields with regard to legal fitness to drive in the UK, the IVF "passes" some individuals currently classed as unfit to drive due to paracentral scotomata of non-glaucomatous origin. The suitability of the UFOV for assessing crash risk in those with visual field loss is questionable.

Effects of vaccine dose, virus challenge dose and interval from vaccination to challenge on protection of broiler chickens against Marek's disease virus challenge.

OBJECTIVES: To examine the effects of varying the doses of turkey herpesvirus (HVT) vaccine and Marek's disease virus (MDV) challenge at two intervals after vaccination on the protection of chickens against challenge with MDV. DESIGN AND PROCEDURE: Experiment 1, a dose response study, consisted of 11 doses of HVT vaccine administered at hatch followed by challenge with 100 plaque forming units (pfu) of MDV 5 days post vaccination. Experiment 2, a 2 x 6 x 2 factorial design, included two HVT vaccine types, six different doses of HVT vaccine and 50 pfu and 200 pfu of MDV challenge 2 days post vaccination. All chickens were reared up to day 56 post challenge when all survivors were killed humanely. Dead and killed chickens were examined for gross MD tumours. RESULTS: Experiment 1 showed a significant positive linear relationship between dose of HVT vaccine and protective index in chickens challenged 5 days post vaccination. However the range of protective index observed was limited. In Experiment 2 neither HVT vaccine provided significant protection at any dose. There was no significant effect of vaccine type or MDV challenge dose on overall protection against challenge. Chickens challenged with 200 pfu of MDV had significantly higher mortality and MD incidence than those with 50 pfu. CONCLUSIONS: HVT vaccine dose had a significant impact on protective index, but vaccination to challenge interval appeared to have greater impact on the protective efficacy of vaccination. A fourfold increase in challenge dose increased mortality rate and incidence of MD.

Visual attention and the transition from novice to advanced driver.

Inexperienced drivers are particularly vulnerable to road traffic accidents, and inattention emerges as a factor in these accidents. What do these drivers attend to and how can their observation skills be developed? When drivers scan the road around them, differences are observed as function of driving experience and training, with experienced drivers increasing their visual scanning on roadways of increasing complexity. Trained police drivers showed this effect of increased scanning even more than experienced drivers. This suggests that the driver's understanding of the task develops with experience, such that roads that demand increased monitoring (e.g. interweaving traffic on a multi-lane highway) receive more extensive scanning than roads that are simpler (e.g. light traffic on a straight rural road). Novice drivers do not show this sensitivity to road complexity, suggesting that they fail to attend to potential dangers involving the behaviour of other road users. Encouragingly, a simple training intervention can increase the visual scanning of novices.

Monitoring Migration and Measuring Biomass in Benthic Biofilms: The Effects of Dark/far-red Adaptation and Vertical Migration on Fluorescence Measurements.

Pulse modulated fluorescence has increasingly been used as an ecological tool to examine changes in the vertical distribution of microphytobenthic cells within the upper layers of estuarine sediments (most often using the minimum fluorescence yield F(o)) as well as to indicate the health of the community (using the maximum PS II quantum efficiency F(v)/F(m)). However, the practicalities of in situ measurements, often dictates that short dark adaptation periods must be used ( approximately 15 min). The use of far-red light as an alternative to dark adaptation was investigated in natural migratory microphytobenthic biofilms and artificial non-migratory biofilms. Prolonged periods of darkness ( approximately 24 h) were not adequate to achieve 'true' measurements of F(o) and F(v)/F(m), which require complete oxidation of Q(A) and full reversal of non-photochemical quenching (NPQ). In some instances, stable values were only achieved using far-red light. Prolonged exposure to dark/far-red light led to a downwards migration of cells in natural assemblages, as seen by a reduction in both F(o) and the maximum fluorescence yield (F(m)). In non-migratory biofilms, F(m) increased in the dark and far-red treatments, indicating a reversal of NPQ, whereas F(o) decreased in far-red light but increased in the dark. It is suggested that far-red light and darkness differentially affected the balance between NPQ reversal and Q(A) oxidation that lead to the measured F(o) yield. The use of far-red light as an alternative to dark adaptation is discussed and the implications of short (e.g., 15 min) dark adaptation times used in situ are discussed with reference to the vertical migration of cells within sediment biofilms.

The role of herbicides in the erosion of salt marshes in eastern England.

Laboratory studies and field trials were conducted to investigate the role of herbicides on saltmarsh vegetation, and their possible significance to saltmarsh erosion. Herbicide concentrations within the ranges present in the aquatic environment were found to reduce the photosynthetic efficiency and growth of both epipelic diatoms and higher saltmarsh plants in the laboratory and in situ. The addition of sublethal concentrations of herbicides resulted in decreased growth rates and photosynthetic efficiency of diatoms and photosynthetic efficiency of higher plants. Sediment stability also decreased due to a reduction in diatom EPS production. There was qualitative evidence that diatoms migrated deeper into the sediment when the surface was exposed to simazine, reducing surface sediment stability by the absence of a cohesive biofilm. Sediment loads on leaves severely reduced photosynthesis in Limonium vulgare. This, coupled with reduced carbon assimilation from the effects of herbicides, could have large negative consequences for plant productivity and over winter survival of saltmarsh plants. The data support the hypothesis that sublethal herbicide concentrations could be playing a role in the increased erosion of salt marshes that has occurred over the past 40 years.

Poor systemic antibody response after vaccination of commercial broiler breeders with Mycoplasma gallisepticum vaccine ts-11 not associated with susceptibility to challenge.

A live attenuated Mycoplasma gallisepticum vaccine, ts-11, has been used for control of M gallisepticum in several countries. The rapid serum agglutination test is usually used as an indicator of flock response to vaccination; however, in some flocks, the detected response may be weak or absent. We investigated whether the low level, or lack, of systemic antibodies in ts-11-vaccinated flocks is correlated with susceptibility to infection after challenge with a virulent M. gallisepticum strain. Birds from 2 separate ts-11-vaccinated commercial flocks with no, or weak, rapid serum agglutination responses (at 11 or 14 wk postvaccination) were randomly selected and subjected to aerosol challenge with either M gallisepticum strain Ap3AS or sterile mycoplasma broth. A group of nonvaccinated specific-pathogen-free chickens at similar age were also exposed to aerosolization with M. gallisepticum strain Ap3AS and used as positive controls. Postmortem examination of the birds, performed 2 wk after challenge, revealed no significant difference in microscopic tracheal lesions or mucosal thicknesses between the ts-11-vaccinated field birds irrespective of their aerosolization treatment. However, both microscopic tracheal lesions and tracheal mucosal thicknesses of nonvaccinated challenged birds were significantly greater than those of ts-11 vaccinates. Hence, broiler breeders vaccinated in the field showed significant protection against virulent M. gallisepticum challenge even when no serum antibody was detected by rapid serum agglutination test. These results reveal that seroconversion detected by rapid serum agglutination test after ts-11 vaccination is not a reliable predictor of protection against M. gallisepticum infection. The possible significance of local antibody response and cell-mediated immunity against M. gallisepticum infection is discussed.

Rapid assessment of macro algal cover on intertidal sediments in a nutrified estuary.

Macroalgal blooms have been considered to be an indicator of eutrophication. A new and rapid method is described for the assessment of macroalgal cover in the intertidal zone of estuaries. Macroalgal cover in the intertidal of the nutrient-enriched River Deben estuary was found to reach a maximum of 50% coverage, but this varied seasonally with the highest percentage cover during June and July. Macro-algae mats were particularly associated with areas of hard substrata providing suitable attachment points, rather than with the nutrient concentrations along the estuary. The occurrence of macroalgae may be more related to the substrate than to the nutrient status of the estuary.

The potential for phosphorus release across the sediment-water interface in an eutrophic reservoir dosed with ferric sulphate.

Alton Water, Suffolk, UK is a pumped storage reservoir that has a history of cyanobacterial blooms. Dosing of the input water with ferric sulphate to control external phosphorus loading has occurred since 1983. A detailed study of the sediment chemistry of the site was carried out between May 1995 and July 1997. Sequential phosphorus fraction analysis indicated a decrease along the length of the reservoir in sediment labile phosphorus content from 0.62 to 0.08 mg P g-1 dw and iron-bound phosphorus content from 3.22 to 0.46 mg P g-1 dw. These gradients positively correlated with water column chlorophyll a concentrations reported in a parallel study. Labile and iron-bound sediment phosphorus contents were in a dynamic equilibrium due to diffusional release, contributing to internal loading to the water column. Equilibrium phosphorus concentrations (EPC) determined from phosphorus adsorption capacity (PAC) experiments were lower inside the bunded region (0.01-0.03 mg P-PO4 l-1) where iron content was greatest compared to outside the bund (0.15-0.20 mg P-PO4 l-1) suggesting greater potential for diffusional release of phosphorus at the latter site. PAC experiments indicated that anaerobic and pH-mediated loadings were of less importance than diffusional release, although the latter may have contributed to internal loading in the main reservoir. Sulphate concentrations may act to increase the potential for anaerobic internal loading near to the pumped input in microstratified sediment. Sediment iron content decreased from 250 +/- 13.1 to 51 +/- 4.0 mg Fe g-1 dw across the line of a constructed bund at the north-west end near to the pumped input, which indicated successful control of dispersal of the fine ferric floc. The management implications with regard to phosphorus loadings indicated by these results are discussed.

Driving experience and the functional field of view.

Research has suggested that novice drivers have different search strategies compared with their more experienced counterparts, and that this may contribute to their increased accident liability. One issue of concern is whether experienced drivers have a wider field of peripheral vision than less experienced drivers. This study attempted to distinguish between people of varying driving experience on the basis of their functional fields of view. Participants searched video clips taken from a moving driver's perspective for potential hazards while responding to peripheral target lights. Hit rates for peripheral targets decreased for all participant groups as processing demands increased (i.e. when hazards occurred) and as the eccentricity of the target increased, though there was no interaction. An effect of experience was also found which suggests that this paradigm measures a perceptual skill or strategy that develops with driving experience.

Visual search of driving situations: danger and experience.

Previous research on visual search in driving suffers from a number of problems: small sample sizes, a concentration on mundane situations, and a failure to link results to more general psychological theory. The study reported in this paper addresses these issues by recording the eye movements of a large sample of drivers while they watched films of dangerous driving situations and comparing the findings with those from more general studies on scene perception. Stimuli were classified according to the types of road shown and the degree of danger present in the scenes. Two groups of subjects took part, fifty-one young novice drivers who had just gained a full driving licence and twenty-six older more experienced drivers. Dangerous situations were characterised by a narrowing of visual search, shown by an increase in fixation durations, a decrease in saccade angular distances, and a reduction in the variance of fixation locations. These effects are similar to the concept of 'attention focusing' in traumatic situations as it is described in the literature on eyewitness memory. When road types are compared, the least visually complex rural roads attracted the longest fixation durations and the shortest angular saccade distances, while the most visually complex urban roads attracted the greatest spread of search but the shortest fixation durations. Differences between the groups of subjects were also present. Novices had longer fixation durations than experienced drivers, particularly in dangerous situations. Experienced drivers also fixated lower down and had less vertical variance in fixation locations than novices.

Studying expertise in music reading: use of a pattern-matching paradigm.

Two experiments are described that make use of a pattern-matching paradigm to investigate perceptual processing of music notation. In Experiment 1, it is reported that the speed of comparing two visually presented musical sequences is related to the sight-reading skill of the subjects. The effect of the temporal and pitch structure of the comparison stimuli is also assessed. In Experiment 2, eye-movement recordings were taken as subjects performed the task. These data demonstrated that more experienced musicians are able to perform the comparisons with fever, and shorter, glances between the patterns. These and other findings suggest that skilled sight-reading is associated with an ability to rapidly perceive notes or groups of notes in the score, and confirm that the pattern-matching paradigm is a useful tool in examining expertise in music reading.

Eye fixations, speech rate and bilingual digit span: numeral reading indexes fluency not word length.

The present study examined whether the reading of language-neutral stimuli, as numerals are, at maximal speed by bilinguals indexes processes related to fluency rather than differences in articulation time between languages. We tested two groups of bilinguals that spoke the same languages (Finnish and Swedish) but whose mother tongues were different and obtained measures of Arabic numeral processing by monitoring eye movements. These measures were contrasted with articulation and numeral reading estimates of word length. The results indicated that Finnish- and Swedish-dominant bilinguals had shorter gaze durations and shorter reading times in their respective dominant languages, whereas both groups articulated digits faster in Swedish than Finnish. The Swedish-dominant group had a larger digit span in Swedish, whereas digit span was marginally greater in Finnish than Swedish for the Finnish-dominant group. The finding that numeral reading was influenced by cognitive loads independent of articulation, thus, moderated the view that bilingual digit span effects are mediated exclusively by variation in word length between languages.

Inspecting asymmetric presentations of words differing in informational and morphemic structure.

Are laterality effects in visual word recognition a product of functional asymmetry of the cerebral hemispheres or a result of stimulus inspection factors such as asymmetric eye fixations before and during visual presentation, different movement time to asymmetric targets, an interaction of the informative components, or morphemic structure of a target with a drop in acuity with distance? Two experiments were conducted to address these questions. Experiment One recorded the inspection of prefixed and matched control words presented unilaterally for 200 msec. Experiment Two displayed suffixed words and their appropriate controls. The words also varied in their information distribution. Reader's eye movements were monitored while they inspected the target words in preparation for a synonym judgement task. Measures of the location of the initial fixation on the screen showed that subjects were not biased to the right visual field prior to stimulus onset. When only those occasions in which the subject had correctly fixated the central cursor were analyzed, a robust right visual field advantage was observed on many of the target inspection time measures, but no reliable difference was observed for faster movement time to the right visual field's target compared to that observed in the left visual field. Neither the distribution of information within the word nor its morphemic structure affected the pattern of any inspection time measures. Consequently, such factors can be ruled out as threats to the hypothesis concerned with the functional asymmetry of the cerebral hemispheres.

Individual differences in reading subprocesses: relationships between reading ability, lexical access, and eye movement control.

This study examines the relationship between individual reading subprocesses and general reading ability in college students. The reading measures included eye movements while reading a passage, lexical decision latencies, comprehension, and vocabulary size. The results indicate that a distinct relation exists between reading speed and fixation behaviour associated with regressions through a text. About half the variability in comprehension scores can be predicted by subjects' performance on nonword lexical decisions, gaze durations, and vocabulary scores. These findings are discussed with reference to past studies using similar reading measures.

Enhancing presentation dynamics through voice projection.

Teaching adult learners requires ongoing preparation and commitment to excellence by adult educators. Central to the educational process is the educator's teaching style. One of the most powerful yet frequently underdeveloped qualities is the educator's voice. The authors present the Voice Projection Instrument as a personal development tool for adult educators to assess and enhance their speaking effectiveness in the classroom.

Differences in lingual vibrotactile thresholds of sensitivity using adjusted vs fixed methods of vibrator probe-contactor alignment.

The purpose of this study was to compare lingual vibrotactile thresholds of sensitivity obtained using individual subject adjustments of vibrator probe-contractor to lingual surface, with thresholds obtained using a fixed vibrator probe-contactor position across 30 subjects ranging in age from 18 to 26 yr. (M age = 20 yr.). Analysis showed minor (less than 5 dB SPL) differences in lingual vibrotactile thresholds of sensitivity between the two methods of vibrator probe-contactor alignment. For research purposes, the vibrator probe-contactor assembly should be adjusted to each individual's lingual surface, but for clinical-behavioral purposes, it might be sensible to use a fixed vibrator probe-contactor position across subjects, especially when performing research on young children, older adults, and the communicatively handicapped.