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MALDI-TOF MS detection of carbapenemase activity in clinical isolates of Enterobacteriaceae spp., Pseudomonas aeruginosa, and Acinetobacter baumannii compared against the Carba-NP assay.
Chong, Patrick M; McCorrister, Stuart J; Unger, Mark S; Boyd, David A; Mulvey, Michael R; Westmacott, Garrett R.
J Microbiol Methods ; 111: 21-3, 2015 Apr.
Article in English | MEDLINE | ID: mdl-25644285

ABSTRACT

MALDI-TOF MS detection of carbapenemase-activity in Gram-negative bacteria was compared against the Carba-NP assay. MALDI-TOF MS detected activity from 99% of the strains, from all types of carbapenemase (200/202), while Carba-NP assays detected activity from 85% (45/53) of the tested isolates and could not consistently identify OXA- or GES carbapenemase activity.


Subject(s)
Acinetobacter baumannii/enzymology , Bacterial Proteins/metabolism , Enterobacteriaceae/enzymology , Pseudomonas aeruginosa/enzymology , Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods , beta-Lactamases/metabolism , Bacteriological Techniques , Mass Spectrometry
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