ABSTRACT
n-Hexane, ethyl acetate, ethanolic and aqueous extracts of the bulbs and aerial parts of Sternbergia sicula and Sternbergia lutea were tested for their antimicrobial and antifungal activity.
Subject(s)
Anti-Bacterial Agents/pharmacology , Antifungal Agents/pharmacology , Liliaceae , Phytotherapy , Plant Extracts/pharmacology , Anti-Bacterial Agents/administration & dosage , Anti-Bacterial Agents/therapeutic use , Antifungal Agents/administration & dosage , Antifungal Agents/therapeutic use , Candida albicans/drug effects , Gram-Negative Bacteria/drug effects , Gram-Positive Bacteria/drug effects , Humans , Microbial Sensitivity Tests , Plant Components, Aerial , Plant Extracts/administration & dosage , Plant Extracts/therapeutic use , Plant RootsABSTRACT
A new indole alkaloid, galanthindole, was isolated from Galanthus plicatus ssp. byzantinus (Amaryllidaceae), a plant native to northwestern Turkey. Incorporating a non-fused indole ring, galanthindole may represent the prototype of a new subgroup of the Amaryllidaceae alkaloids. Two other bases, (+)-11-hydroxyvittatine and hordenine, are also reported from the same plant.
Subject(s)
Amaryllidaceae Alkaloids , Antineoplastic Agents, Phytogenic/chemistry , Galanthus , Indole Alkaloids/chemistry , Phytotherapy , Plant Extracts/chemistry , Alkaloids/chemistry , Humans , Indoles/chemistry , Magnetic Resonance SpectroscopyABSTRACT
The interaction of lycorine (LYC) with calf thymus double stranded DNA (dsDNA) and calf thymus single stranded DNA (ssDNA) was studied electrochemically based on the oxidation signals of guanine and adenine by using differential pulse voltammetry (DPV) at carbon paste electrode (CPE) and pencil graphite electrode (PGE). As a result of the interaction of LYC with DNA, the voltammetric signal of guanine and adenine greatly decreased. The changes in the experimental parameters such as the concentration of LYC, and the accumulation time of LYC were studied by using DPV with PGE. The interaction of LYC with synthetic polynucleotides, such as poly[G] was also observed. The interaction of LYC with dsDNA was also observed at PGE in solution phase. In addition, the detection limit and the reproducibility was determined by using both electrochemical transducers. The application of electrochemical methods on the interactions between DNA and DNA targeted agent were explored.
