ABSTRACT
Survival patterns of NBL/Up mice exposed to 675-800 R of total-body x-radiation depended on direction and rate of exposure. Homogeneous dual exposures administered at 126 R/minute were more effective than those administered at 63 R/minute. Dorsal and dual exposures at 63 R/minute were equally effective, while absorption reduced the effectiveness of ventral exposures. Since cellular repair of hematopoietic tissue was initiated only during exposure, increased homogeneity without increased exposure rate would not increase effectiveness. With rate, direction, and homogeneity influencing the effectiveness of total-body exposures, fallacies inherent in converting total-body exposures to absorbed tissue dose became apparent.
Subject(s)
Radiotherapy/methods , Whole-Body Irradiation/methods , Animals , Energy Transfer , Female , Mice , Radiotherapy/adverse effects , Radiotherapy Dosage , Whole-Body Irradiation/adverse effectsABSTRACT
Lethally irradiated parental strain male and female mice were inoculated with marrow from reciprocal hybrid donors of the same sex. Hybrid cells homozygous for the H-2k genotype could recognize the quantitatively greater antigenicity of the parental strain CBA male recipients only. The more severe reaction occurred when the maternal strain of the hybrid donor was allogeneic to the recipient, which indicates the maternal effect on the antigen recognition system. This demonstration of a maternal influence on the expression of paternally derived tissue antigens made it possible to attribute the secondary disease syndrome to a hybrid-versus-parental strain reaction. The apparent differences in mortality between male and female recipients could not be attributable to X or Y chromosome disparity without additional transfers of appropriate marrow from males to females and females to males. In all cases, the parental strain-versus-hybrid reaction resulted in greater mortality than that observed in the hybrid-versus-parental strain reaction.
Subject(s)
Bone Marrow Cells , Bone Marrow Transplantation , Graft vs Host Reaction , Histocompatibility , Animals , Female , Hybridization, Genetic , Male , Mice , Mice, Inbred AKR , Mice, Inbred CBA , Sex Factors , Transplantation, HomologousABSTRACT
Lethally irradiated perental strain mice were inoculated with marrow from reciprocal hybrid donors. Hybrid cells could recognize the quantitatively greater antigenicity of parental strain recipients. The severity of the hybrid-versus-parental strain and parental strain-versus-hybrid reactions was compared. There was a significantly less severe reaction when DBA/2 mice were inoculated with (DBA/2female X C57BLmale)F1 arrow than with (C57BL X DBA/2)F1 marrow, which indicated a maternal influence on antigen recognition. The reaction of (DBA/2 X C57BL)F1 against DBA/2 recipients was significantly less severe than the DBA/2 reaction against (DBA/2 X C57BL)F1 recipients. All reactions involving C57BL were equally severe and more lethal than those involving DBA/2 mice. The strength of these reactions was a function of the relative tissue antigenicity and responsiveness of the parental strains of the hybrid cross. Both the expression of tissue antigens and the ability to recognize and respons to them are influenced by the maternal strain of the hybrid cross.
Subject(s)
Bone Marrow Cells , Bone Marrow/immunology , Histocompatibility Antigens , Animals , Bone Marrow Transplantation , Hybridization, Genetic , Mice , Mice, Inbred C57BL , Mice, Inbred DBAABSTRACT
Lethally irradiated male and female hybrids, parental strains, and the third party strain A mice were inoculated with reciprocal hybrid marrow from donors of the same sex. A graft-versus-host reaction was produced against H-Y antigens of reciprocal hybrids. Although the late mortality in inbred strain males was generally greater than that of females, without further tests this difference was not automatically attributable to an X or a Y chromosome disparity. Hybrids did not recognize the qualitative difference in antigenicity of their reciprocals, but they were able to recognize the quantitatively greater antigenicity of their parental strains, and a hybrid-versus-parental strain reaction occurred. The graft-versus-host reaction in the third party strain A mice was significantly more severe than that produced by hybrid marrow in their reciprocal hybrid recipients, but significantly less severe than that produced in the parental strains. The relationship between antigenicity and responsiveness was such that the severity of the graft-versus-host reactions masked the maternal influences observed with other reciprocal hybrids.
Subject(s)
Bone Marrow Cells , Bone Marrow Transplantation , Graft vs Host Reaction/mortality , Hybridization, Genetic , Animals , Bone Marrow/immunology , Female , Histocompatibility Antigens , Male , Mice , Mice, Inbred A , Mice, Inbred C3H , Sex Chromosomes , Transplantation, HomologousSubject(s)
Antigens , Epitopes , Animals , Bone Marrow Cells , Bone Marrow Transplantation , Female , Graft Rejection , Graft vs Host Reaction , Histocompatibility Antigens , Hybridization, Genetic , Immunogenetics , Male , Mice , Radiation Chimera , Sex Factors , Skin Transplantation , Transplantation, IsogeneicABSTRACT
The response of marrow from virgin female donors to the antigens controlled by the Y chromosome of syngeneic male recipients varied with different strains of mice and was independent of their H-2 genotype. Marrow from syngeneic multiparous donors might produce a greater response to male antigens than that observed with marrow from virgin females. The sensitization of the mother to her male progeny indicated that these antigens were expressed before parturition. The reduced responsiveness of mothers to some alloantigens and the accompanying increased responsiveness to male antigens suggested a difference in the time during embryonic development when these antigens were expressed.
