Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Insect Biochem Mol Biol ; 34(9): 963-9, 2004 Sep.
Article in English | MEDLINE | ID: mdl-15350615

ABSTRACT

To clarify the molecular mechanisms of metamorphosis, we analyzed the Broad-Complex (BR-C) gene in the silkworm, Bombyx mori. We cloned cDNAs for the full coding regions of the Z1, Z2, and Z4 isoforms of BR-C. The Z3 zinc finger sequence was found in the 3'UTR of the Z2 isoform. The predicted amino acid sequence showed high homology with Drosophila and Manduca BR-C proteins. Five bacterial artificial chromosome (BAC) clones were screened from a Bombyx BAC library. Restriction enzyme cleavage maps of 170 kb regions were constructed, and a total of 25.8 kb were sequenced. The BAC analysis showed that the 5'UTR of the BR-C gene consists of the first two exons, while the coding region contains a core region domain with five exons and four zinc finger exons in the order Z1, Z4, Z2, and Z3. Expression analysis revealed 9.5, 6.5, and 5.5 kb BR-C transcripts. These increased during the spinning ecdysone peak on day 6 of the fifth instar when pupal commitment occurs in the Bombyx epidermis. In addition, a small amount of BR-C mRNA was detected in the epidermis before this peak. BR-C mRNA was also expressed in the fat body from day 1 in the fourth instar to day 7 in the fifth instar.


Subject(s)
Bombyx/genetics , Insect Proteins/genetics , Metamorphosis, Biological/genetics , Transcription Factors/genetics , Amino Acid Sequence , Animals , Base Sequence , Bombyx/growth & development , Chromosomes, Artificial/genetics , Cloning, Molecular , DNA, Complementary/genetics , Gene Expression Profiling , Insect Proteins/biosynthesis , Larva/genetics , Molecular Sequence Data , Polymerase Chain Reaction , Transcription Factors/biosynthesis , Zinc Fingers
SELECTION OF CITATIONS
SEARCH DETAIL
...