[Epidemiologic characterization of Brucellosis under modern conditions]. / Epidemiologicheskaia kharakteristika brutseleza v sovremennykh usloviiakh.

The spread of brucella infection under modern conditions of cattle breeding were studied with the foci in the Rostov region taken as an example. The main epidemiological features and tendencies of epidemiological importance were established. To find out specific antigen in different environmental objects, the approbation of the enzyme immunoassay test systems was carried out and the possibility of their use at the institutions of the State Sanitary and Epidemiological inspection was shown.

[The isolation of the Vibrio cholerae dermotoxin and the characterization of its biological properties]. / Vydelenie dermotoksina Vibrio cholerae i kharakteristika ego biologicheskikh svoistv.

The dermonecrotic factor (dermotoxin) inducing skin necrosis in rabbits has been isolated from V. cholerae strain B-53-2-38 and partially purified. Dermotoxin has a molecular weight of about 110 kD and possesses pronounced cytotoxic and general toxic action, differing from that of enterotoxin. The introduction of this factor into the blood and peritoneum of laboratory animals causes their death.

[The complex study of the epidemic situation and of the immunological structure of the population in brucellosis foci]. / Kompleksnoe izuchenie épidemicheskoi situatsii i immunologicheskoi struktury naseleniia v ochagakh brutselleza.

As the result of the complex clinico-laboratory examination of 265 cattle breeders working on 5 farms where cases of brucellosis among agricultural animals had been detected, 62 persons (21.8%) were found to have the positive reaction to brucellosis. A strikingly high proportion (38.3%) of infected persons was found among the workers of a cattle-feeding complex. Out of 62 persons with the positive reaction to brucellosis, 32 had different clinical manifestations of the disease. Treatment for all persons who needed it was organized, the rest were placed under dispensary observation. Regular complex clinico-laboratory examinations of cattle breeders to brucellosis were recommended.

[The C antigen of Francisella tularensis]. / C-antigen tuliiaremiinogo mikroba.

A new envelope antigen C, specific for virulent strains of Francisella tularensis, was revealed by immunodiffusion analysis. In contrast to antigens A and P this antigen is common for Francisella and Brucella. C-antigenic lipid fraction was obtained by chloroform-ethanol (1:1) extraction of bacterial slime. This fraction contained carbohydrates (31.6%) without proteins and detected by TLC glycolipid, which proved glycolipid nature of C-antigen. Introduction of C-fraction or alive F. tularensis resulted in accumulation of C. precipitins in blood serum.

[Characteristics of the protease activity of Vibrio cholerae strains and mutants with various biological properties]. / Kharakteristika proteaznoi aktivnosti shtammov i mutantov Vibrio cholerae s razlichnymi biologicheskimi svoistvami.

The study of 65 V. cholerae strains and mutants revealed that protease activity was high in choleragenic strains and mostly low, sometimes even not manifested, in noncholeragenic strains. The use of inhibitors made it possible to determine three types of protease. Choleragenic strains were found to possess all three types of protease, while in noncholeragenic strains only protease of type 1 was detected in very rare cases. In noncholeragenic hemolytic strains and mutants protease of type 3 and in nonhemolytic strains protease of type 2 were mainly detected. Proteases of types 1 and 3 were thermolabile, and protease of type 2 was thermostable. Proteases of types 1 and 2 were found to be correlatively linked with the choleragenicity of V. cholerae and with a number of its properties which determine the pathogenicity of this infective agent.

[Potentials and conditions for the reversion of pathogenic properties of the causative agent of cholera in an experiment]. / Vozmozhnosti i usloviia reversii patogennykh svoistv vozbuditelia kholery v éksperimente.

The passage of V. cholerae noncholerigenic strains and their mutants, both in vitro and in vivo, has demonstrated that strains in which one of such properties as mobility, viability, adhesive, lecithinase and neuraminidase activities, is sharply decreased or lost, are still capable of reversion to cholerigenic forms. V. cholerae strains which have lost two or more of these properties, as well as strains having stable hemolytic activity determined by Greig's test, seem to be incapable of such reversion.

[Assessment of the importance of certain properties for the virulence of Vibrio cholerae and the detection of their correlations]. / Otsenka znacheniia nekotorykh svoistv dlia virulentnosti Vibrio cholerae i vyiavlenie ikh korreliativnykh sviazei.

The study of 65 V. cholerae strains and mutants with different biological characteristics has revealed that their virulence directly correlates with their sensitivity to diagnostic cholera phages, lecithnase activity, viability and inversely correlates with their hemolytic activity. The role of lecithinase activity in a number of properties linked with cholerigenicity has been shown. Inverse correlation between the hemolytic activity of V. cholerae and some properties of this organism has been detected. The characteristics under study have been divided into 3 groups in accordance with correlations between them.

[Chemotaxis of cholera vibrios: a study method and the relation to different substances]. / Khemotaksis kholernykh vibrionov: metodika izucheniia i otnoshenie k razlichnym veshchestvam.

The chemotaxis of V. cholerae in response to 56 different substances (amino acids, carbohydrates, salts, etc.) has been studied by the methods of visual observation and quantitative determination. Attractants, neutral substances and one repellent have been revealed. Adler's method (1973) has been modified with regard to the requirements for the working procedures in handling the causative agents of highly dangerous infections.

[Adhesive properties of Vibrio cholerae strains and mutants with varying biological characteristics]. / Adgezivnye svoistva shtammov i mutantov kholernykh vibrionov s razlichnoi biologicheskoi kharakteristikoi.

V. cholerae strains and mutants devoid of adhesive activity and possessing very high adhesive activity were found in the collection under study. As a rule, cholerigenic strains possessed high or moderate adhesive activity, while in noncholerigenic strains the coefficient of adhesiveness varied over a wide range. The existence of a definite pronounced correlation between lecithinase activity and adhesiveness was established. Hemolysin-producing strains were found to possess high adhesive activity considerably more frequently than nonhemolytic strains.