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1.
J Cell Sci ; 132(10)2019 05 16.
Article in English | MEDLINE | ID: mdl-30992345

ABSTRACT

Cell-cell communication is essential for tissue homeostasis, but its contribution to disease prevention remains to be understood. We demonstrate the involvement of connexin 43 (Cx43, also known as GJA1) and related gap junction in epithelial homeostasis, illustrated by polarity-mediated cell cycle entry and mitotic spindle orientation (MSO). Cx43 localization is restricted to the apicolateral membrane of phenotypically normal breast luminal epithelial cells in 3D culture and in vivo Chemically induced blockade of gap junction intercellular communication (GJIC), as well as the absence of Cx43, disrupt the apicolateral distribution of polarity determinant tight junction marker ZO-1 (also known as TJP1) and lead to random MSO and cell multilayering. Induced expression of Cx43 in cells that normally lack this protein reestablishes polarity and proper MSO in 3D culture. Cx43-directed MSO implicates PI3K-aPKC signaling, and Cx43 co-precipitates with signaling node proteins ß-catenin (CTNNB1) and ZO-2 (also known as TJP2) in the polarized epithelium. The distribution of Cx43 is altered by pro-inflammatory breast cancer risk factors such as leptin and high-fat diet, as shown in cell culture and on tissue biopsy sections. The control of polarity-mediated quiescence and MSO may contribute to the tumor-suppressive role of Cx43.


Subject(s)
Breast/cytology , Breast/metabolism , Cell Polarity/physiology , Connexin 43/metabolism , Spindle Apparatus/metabolism , Cell Communication/physiology , Cell Differentiation/physiology , Cell Line , Epithelium/metabolism , Female , Gap Junctions/metabolism , Humans , Mitosis/physiology , Phosphatidylinositol 3-Kinases/metabolism , Signal Transduction , Zonula Occludens-2 Protein/metabolism , beta Catenin/metabolism
2.
Neuroscience ; 186: 1-12, 2011 Jul 14.
Article in English | MEDLINE | ID: mdl-21557989

ABSTRACT

We investigated the role of the vesicular acetylcholine transporter in the mechanism of non-quantal (non-vesicular) secretion of neurotransmitter in the neuromuscular synapse of the rat diaphragm muscle. Non-quantal secretion was estimated electrophysiologically by the amplitude of end-plate hyperpolarization after inhibition of cholinesterase and nicotinic receptors (H-effect) or measured by the optical detection of acetylcholine in the bathing solution. It was shown that 1 mM methyl-ß-cyclodextrin (MCD) reduced both endocytosis and, to much lesser extent, exocytosis of synaptic vesicles (SV) thereby increasing non-quantal secretion of acetylcholine with a concurrent decrease in axoplasm pH. During high-frequency stimulation of the motor nerve, that substantially increases vesicles exocytosis, the non-quantal secretion was further enhanced if the endocytosis of SV was blocked by MCD. In contrast, non-quantal secretion of acetylcholine did not increase when the MCD-treated neuromuscular preparations were superfused with either vesamicol, an inhibitor of vesicular transporter of acetylcholine, or sodium propionate, which decreases intracellular pH. These results suggest that the proton-dependent, vesamicol-sensitive vesicular transporters of acetylcholine, which become inserted into the presynaptic membrane during SV exocytosis and removed during endocytotic recycling of SV, play the major role in the process of non-quantal secretion of neurotransmitter.


Subject(s)
Acetylcholine/metabolism , Endocytosis/physiology , Neuromuscular Junction/metabolism , Presynaptic Terminals/metabolism , Vesicular Acetylcholine Transport Proteins/physiology , beta-Cyclodextrins/pharmacology , Animals , Endocytosis/drug effects , Male , Neural Inhibition/drug effects , Neural Inhibition/physiology , Neuromuscular Junction/drug effects , Presynaptic Terminals/drug effects , Rats , Rats, Wistar , Synaptic Transmission/drug effects , Synaptic Transmission/physiology , Up-Regulation/drug effects , Up-Regulation/physiology , Vesicular Acetylcholine Transport Proteins/antagonists & inhibitors
3.
Bull Exp Biol Med ; 147(5): 583-6, 2009 May.
Article in English, Russian | MEDLINE | ID: mdl-19907744

ABSTRACT

Exogenous adenosine triphosphoric acid produces a biphasic effect on the resting membrane potential of muscle fibers in rat diaphragm. Depolarization of the sarcolemma observed 10 min after application of adenosine triphosphoric acid results from activation of Na(+)/K(+)/2Cl(-) cotransport. The increase in chloride cotransport is related to activation of postsynaptic P2Y receptors and protein kinase C. Repolarization of the membrane develops 40 min after treatment with adenosine triphosphoric acid and after 50 min the resting membrane potential almost returns the control level. This increase in the resting membrane potential of the sarcolemma is probably associated with activation of the Na(+)/K(+) pump and increase in membrane permeability for chlorine ions in response to long-term activity of Cl(-) cotransport. Thus, adenosine triphosphoric acid co-secreted with acetylcholine in the neuromuscular synapse probably plays a role in the regulation resting membrane potential and cell volume of muscle fibers.


Subject(s)
Adenosine Triphosphate/pharmacology , Chlorides/metabolism , Muscle Fibers, Skeletal/drug effects , Muscle Fibers, Skeletal/metabolism , Potassium/metabolism , Sodium/metabolism , Acetylcholine/metabolism , Animals , Biological Transport/drug effects , Male , Membrane Potentials/drug effects , Protein Kinase C/metabolism , Rats , Receptors, Purinergic P2/metabolism , Sarcolemma/drug effects , Sarcolemma/metabolism
4.
Neuroscience ; 150(4): 915-25, 2007 Dec 19.
Article in English | MEDLINE | ID: mdl-18031937

ABSTRACT

Regeneration of neuronal circuits cannot be successful without restoration of full function, including recovery of behavioral plasticity, which we have found is delayed after regeneration of specific synapses. Experiments were designed to measure neuronal changes that may underlie recovery of function. Sensitization of the leech withdrawal reflex is a non-associative form of learning that depends on the S-interneuron. Cutting an S-cell axon in Faivre's nerve disrupted the capacity for sensitization. The S-cell axon regenerated its electrical synapse with its homologous cell after 3-4 weeks, but the capacity for sensitization was delayed for an additional 2-3 weeks. In the present experiments another form of non-associative conditioning, dishabituation, was also eliminated by S-cell axotomy; it returned following regeneration. Semi-intact preparations were made for behavioral studies, and chains of ganglia with some skin were used for intracellular recording and skin stimulation. In both preparations there was a similar time-course, during 6 weeks, of a lesion-induced decrease and delayed restoration of both S-cell action potential threshold to depolarizing pulses and S-cell firing in response to test stimuli. However, the ability of sensitizing stimuli to decrease S-cell threshold and enhance S-cell activity in response to test stimuli did not fully return after regeneration, indicating that there were lasting changes in the circuit extending beyond the period necessary for full recovery of behavior. Intracellular recordings from the axotomized S-cell revealed a shift in the usual balance of excitatory and inhibitory input, with inhibition enhanced. These results indicate that loss of behavioral plasticity of reflexive shortening following axotomy in the S-cell chain may be related to reduced S-cell activity, and that additional processes underlie full recovery of sensitization of the whole body shortening reflex.


Subject(s)
Interneurons/cytology , Nerve Net/cytology , Nerve Regeneration/physiology , Neuronal Plasticity/physiology , Recovery of Function/physiology , Synapses/physiology , Action Potentials/physiology , Action Potentials/radiation effects , Animals , Axotomy/methods , Behavior, Animal , Dose-Response Relationship, Radiation , Electric Stimulation/methods , In Vitro Techniques , Interneurons/physiology , Leeches , Models, Neurological , Recovery of Function/radiation effects , Reflex/physiology , Synaptic Transmission/physiology , Time Factors
5.
Bull Exp Biol Med ; 142(6): 683-4, 2006 Dec.
Article in English, Russian | MEDLINE | ID: mdl-17603669

ABSTRACT

N-acetylaspartylglutamate prevents the denervation-induced increase in the volume of muscle fibers in rat diaphragm, the phenomenon being more pronounced for the hydrolysable isomer. The effect of dipeptide manifested against the background of blockade of metabotropic glutamate receptors. It was hypothesized that N-acetylaspartylglutamate is involved in the regulation of the volume of skeletal muscle fibers via activation of ionotropic receptors by both dipeptide and glutamate molecules.


Subject(s)
Cell Size/drug effects , Dipeptides/pharmacology , Muscle Fibers, Skeletal/drug effects , Muscle, Skeletal/cytology , Animals , Excitatory Amino Acid Agonists/pharmacology , Excitatory Amino Acid Antagonists/pharmacology , Glutamates/pharmacology , Male , Muscle Denervation , Muscle Fibers, Skeletal/cytology , Muscle Fibers, Skeletal/metabolism , Muscle, Skeletal/innervation , Muscle, Skeletal/metabolism , N-Methylaspartate/pharmacology , Neurosurgical Procedures , Rats , Receptors, Metabotropic Glutamate/agonists , Receptors, Metabotropic Glutamate/antagonists & inhibitors , Receptors, Metabotropic Glutamate/physiology
6.
Ross Fiziol Zh Im I M Sechenova ; 90(8): 957-67, 2004 Aug.
Article in Russian | MEDLINE | ID: mdl-15552363

ABSTRACT

The paper is devoted to the analysis of evidence pointing to presence of glutamatergic modulation of vertebrate neuromuscular transmission. The data on the glutamate's origin and release in the endplate region as well as on the presence of specific glutamate receptors are discussed. The effects of glutamate on different types of acetylcholine secretion in the synapses of amphibians and mammals are described. The question of possible physiological role of glutamatergic modulation of neuromuscular transmission is discussed.


Subject(s)
Glutamic Acid/physiology , Neuromuscular Junction/physiology , Synaptic Transmission/physiology , Vertebrates/physiology , Acetylcholine/physiology , Animals , Glutamic Acid/pharmacology , Receptors, Glutamate/drug effects , Receptors, Glutamate/physiology , Synapses/physiology
7.
Bull Exp Biol Med ; 135(2): 120-2, 2003 Feb.
Article in English | MEDLINE | ID: mdl-12802412

ABSTRACT

Denervation of rat phrenic muscle or block of NO-synthase in vivo increased the cross-section area of muscle fibers and decreased membrane resting potential. Oxotremorine prevented the development of denervation-induced or denervation-like (i.e. induced by NO-synthase blockade) membrane depolarization and increase of the cross-sectional area of muscle fibers. Pirenzepine abolished the effects of oxotremorine. It was concluded that non-quantal acetylcholine can be involved in the regulation of skeletal muscle fiber volume via activation of M1 muscarinic receptors followed by NO synthesis.


Subject(s)
Cell Size , Membrane Potentials/drug effects , Muscarinic Agonists/pharmacology , Muscle Fibers, Skeletal/drug effects , Muscle, Skeletal/cytology , Nitric Oxide Synthase/antagonists & inhibitors , Oxotremorine/pharmacology , Acetylcholine/metabolism , Animals , In Vitro Techniques , Membrane Potentials/physiology , Muscarinic Antagonists/pharmacology , Muscle Fibers, Skeletal/cytology , Muscle Fibers, Skeletal/metabolism , Muscle, Skeletal/metabolism , Nitric Oxide Synthase/metabolism , Pirenzepine/pharmacology , Rats
8.
Neurosci Behav Physiol ; 32(6): 577-82, 2002.
Article in English | MEDLINE | ID: mdl-12469883

ABSTRACT

Experiments on rat diaphragm muscles showed that glutamate (10 microM-1 mM) had no effect on the mean frequency, interspike intervals, and amplitude-time characteristics of miniature endplate potentials, but had a suppressive action on non-quantum secretion (the intensity of which was assessed in terms of the H effect). The effect of glutamate was markedly concentration-dependent and was completely overcome by blockade of NMDA receptors, inhibition of NO synthase, and by binding of NO molecules in the extracellular space by hemoglobin. It is suggested that glutamate can modulate the non-quantum release of acetylcholine, initiating the synthesis of NO molecules in muscle fibers via activation of NMDA receptors followed by the retrograde action of NO on nerve terminals.


Subject(s)
Acetylcholine/metabolism , Excitatory Amino Acids/pharmacology , Glutamic Acid/pharmacology , Neuromuscular Junction/drug effects , Neuromuscular Junction/metabolism , Animals , Dizocilpine Maleate/pharmacology , Electric Stimulation , Enzyme Inhibitors/pharmacology , Excitatory Amino Acid Antagonists/pharmacology , Membrane Potentials/drug effects , Motor Endplate/drug effects , Motor Endplate/metabolism , NG-Nitroarginine Methyl Ester/pharmacology , Nitric Oxide/physiology , Nitric Oxide Synthase/antagonists & inhibitors , Nitric Oxide Synthase Type I , Rats
9.
Comp Biochem Physiol B Biochem Mol Biol ; 133(2): 209-20, 2002 Oct.
Article in English | MEDLINE | ID: mdl-12381383

ABSTRACT

We have proposed that N-acetylaspartylglutamate (NAAG) or its hydrolytic product glutamate, is a chemical signaling agent between axons and periaxonal glia at non-synaptic sites in crayfish nerves, and that glutamine is a probable precursor for replenishing the releasable pool of NAAG. We report here, that crayfish central nerve fibers synthesize NAAG from exogenous glutamine. Cellular accumulation of radiolabel during in vitro incubation of desheathed cephalothoracic nerve bundles with [3H]glutamine was 74% Na(+)-independent. The Na(+)-independent transport was temperature-sensitive, linear with time for at least 4 h, saturable between 2.5 and 10 mM L-glutamine, and blocked by neutral amino acids and analogs that inhibit mammalian glutamine transport. Radiolabeled glutamine was taken up and metabolized by both axons and glia to glutamate and NAAG, and a significant fraction of these products effluxed from the cells. Both the metabolism and release of radiolabeled glutamine was influenced by extracellular Na(+). The uptake and conversion of glutamine to glutamate and NAAG by axons provides a possible mechanism for recycling and formation of the axon-to-glia signaling agent(s).


Subject(s)
Axons/metabolism , Dipeptides/biosynthesis , Glutamine/metabolism , Neuroglia/metabolism , Amino Acids/pharmacology , Animals , Astacoidea/metabolism , Astacoidea/physiology , Neurotransmitter Agents/biosynthesis , Radioactive Tracers , Sodium/pharmacology , Tritium
10.
Neuroscience ; 114(3): 699-705, 2002.
Article in English | MEDLINE | ID: mdl-12220571

ABSTRACT

Glutaminase of crayfish axons is believed to participate in recycling of axon-glia signaling agent(s). We measured the activity and properties of glutaminase in crude homogenates of crayfish CNS, using ion exchange chromatography to separate radiolabeled product from substrate. Crayfish glutaminase activity is cytoplasmic and/or weakly bound to membranes and dependent on time, tissue protein, and glutamine concentration. It resembles the kidney-type phosphate-activated glutaminase of mammals in being stimulated by inorganic phosphate and alkaline pH and inhibited by the product glutamate and by the glutamine analog 6-diazo-5-oxo-L-norleucine. During incubation of crayfish CNS fibers in Na(+)-free saline containing radiolabeled glutamine, there is an increased formation of radiolabeled glutamate in axoplasm that is temporally associated with an increase in axonal pH from about 7.1 to about 8.0. Both the formation of glutamate and the change in pH are reduced by 6-diazo-5-oxo-L-norleucine. Our results suggest that crayfish glutaminase activity is regulated by cellular changes in pH and glutamate concentration. Such changes could impact availability of the axon-glia signaling agents glutamate and N-acetylaspartylglutamate.


Subject(s)
Axons/enzymology , Central Nervous System/enzymology , Glutaminase/metabolism , Neuroglia/enzymology , Signal Transduction/physiology , Animals , Astacoidea/enzymology , Axons/drug effects , Central Nervous System/drug effects , Glutaminase/antagonists & inhibitors , Neuroglia/drug effects , Signal Transduction/drug effects
11.
Ross Fiziol Zh Im I M Sechenova ; 88(5): 619-26, 2002 May.
Article in Russian | MEDLINE | ID: mdl-12136730

ABSTRACT

It has been shown that bath application of muscarine delayed the early post-denervation depolarization in the muscle fibers incubated for 3 h in culture medium. The greatest reduction of the post-devervation depolarization was observed with 50 nmol/l muscarine. Atropine, a muscarinic antagonist, clozapine, a specific inhibitor of M1/M5-cholinergic receptors, and nitrocaramiphen, a M1-antagonist, completely removed the hyperpolarizing effect of muscarine. 4-DAMP, a specific inhibitor of M3-cholinergic receptors, himbacine, an antagonist of M2-cholinergic receptors, and tropicamide, a specific inhibitor of M2/M4-cholinergic receptors, failed to prevent the effect of muscarine. A M1/M2 muscarine agonists propargyl and but-2-ynyl esters of arecaidine had apparent muscarine-like effect. Nitrocaramiphen, and not himbacine, prevented the hyperpolarizing effect of these cholinomimetics. It is concluded that muscarine and esters of arecaidine delay the development of early postdenervation depolarization in M1-cholinergic receptors of skeletal muscle.


Subject(s)
Arecoline/analogs & derivatives , Diaphragm/metabolism , Receptors, Muscarinic/metabolism , Acetylcholine/metabolism , Animals , Arecoline/pharmacology , Culture Techniques , Diaphragm/drug effects , Diaphragm/innervation , Male , Membrane Potentials/drug effects , Muscarinic Agonists/pharmacology , Muscarinic Antagonists/pharmacology , Muscle Denervation , Muscle Fibers, Skeletal/metabolism , Rats , Receptors, Muscarinic/drug effects
12.
Ross Fiziol Zh Im I M Sechenova ; 88(11): 1458-66, 2002 Nov.
Article in Russian | MEDLINE | ID: mdl-12587274

ABSTRACT

Cross-sectional area (CSA) of muscle fibers incubated in culture medium 199 for 3 hours dramatically increases, whereas resting membrane potential (RMP) decreases compared to "freshly-isolated" muscles. Both glutamate and sodium nitroprusside prevent these changes. MK-801, a specific inhibitor of NMDA-receptors, eliminates protective effects of glutamate on both CSA and RMP. NO-synthase inhibition in vivo promotes an increase of initial CSA and decrease of mean RMP. Under these conditions, effects of glutamate and sodium nitroprusside on CSA and RMP of denervated muscles are less obvious. It has been concluded that synaptic glutamate is able to participate in regulation of RMP and cell volume in muscle fibers through the activation of postsynaptic NMDA-receptors and muscle NO-synthase.


Subject(s)
Glutamic Acid/pharmacology , Muscle, Skeletal/physiology , Nitric Oxide Synthase/antagonists & inhibitors , Animals , Diaphragm/innervation , Diaphragm/physiology , Dizocilpine Maleate/pharmacology , Enzyme Inhibitors/pharmacology , Male , Membrane Potentials/drug effects , Membrane Potentials/physiology , Muscle Denervation , Muscle Fibers, Skeletal/drug effects , Muscle Fibers, Skeletal/physiology , Muscle, Skeletal/drug effects , NG-Nitroarginine Methyl Ester/pharmacology , Nitric Oxide Donors/pharmacology , Nitroprusside/pharmacology , Rats , Receptors, N-Methyl-D-Aspartate/antagonists & inhibitors
13.
Neuroscience ; 107(4): 697-703, 2001.
Article in English | MEDLINE | ID: mdl-11720792

ABSTRACT

Crayfish nerve fibers incubated with radiolabeled glutamate or glutamine accumulate these substrates and synthesize radioactive N-acetylaspartylglutamate (NAAG). Upon stimulation of the medial giant nerve fiber, NAAG is the primary radioactive metabolite released. Since NAAG activates a glial hyperpolarization comparable to that initiated by glutamate or axonal stimulation through the same receptor, we have proposed that it is the likely mediator of interactions between the medial giant axon and its periaxonal glia. This manuscript reports investigations of possible mechanisms for termination of NAAG-signaling activity. N-acetylaspartyl-[(3)H]glutamate was not accumulated from the bath saline by unstimulated crayfish giant axons or their associated glia during a 30-min incubation. Stimulation of the central nerve cord at 50 Hz during the last minute of the incubation dramatically increased the levels of radiolabeled glutamate, NAAG, and glutamine in the medial giant axon and its associated glia. These results indicate that stimulation-sensitive peptide hydrolysis and metabolic recycling of the radiolabeled glutamate occurred. There was a beta-NAAG-, quisqualate- and 2-(phosphonomethyl)-pentanedioic acid-inhibitable glutamate carboxypeptidase II activity in the membrane fraction of central nerve fibers, but not in axonal or glial cytoplasmic fractions. Inactivation of this enzyme by 2-(phosphonomethyl)-pentanedioic acid or inhibition of N-methyl-D-aspartate (NMDA) receptors by MK801 reduced the glial hyperpolarization activated by high-frequency stimulation. These results indicate that axon-to-glia signaling is terminated by NAAG hydrolysis and that the glutamate formed contributes to the glial electrical response in part via activation of NMDA receptors. Both NAAG release and an increase in glutamate carboxypeptidase II activity appear to be induced by nerve stimulation.


Subject(s)
Dipeptides/pharmacokinetics , Nerve Fibers/metabolism , Neuroglia/physiology , Signal Transduction/physiology , Animals , Astacoidea , Carboxypeptidases/metabolism , Cell Communication/physiology , Cell Membrane/metabolism , Cytoplasm/metabolism , Dizocilpine Maleate/pharmacology , Enzyme Inhibitors/pharmacology , Excitatory Amino Acid Antagonists/pharmacology , Extracellular Space/metabolism , Glutamate Carboxypeptidase II , Neuroglia/cytology , Organophosphorus Compounds/pharmacology , Tritium
14.
Neuroscience ; 106(1): 227-35, 2001.
Article in English | MEDLINE | ID: mdl-11564432

ABSTRACT

Glial cell hyperpolarization previously has been reported to be induced by high frequency stimulation or glutamate. We now report that it also is produced by the glutamate-containing dipeptide N-acetylaspartylglutamate (NAAG), by its non-hydrolyzable analog beta-NAAG, and by NAAG in the presence of 2-(phosphonomethyl)-pentanedioic acid (2-PMPA), a potent inhibitor of the NAAG degradative enzyme glutamate carboxypeptidase II. The results indicate that NAAG mimics the effect of nerve fiber stimulation on the glia. Although glutamate has a similar effect, the other presumed product of NAAG hydrolysis, N-acetylaspartate, is without effect on glial cell membrane potential, as is aspartylglutamate (in the presence of 2-PMPA). The hyperpolarization induced by stimulation, glutamate, NAAG, beta-NAAG, or NAAG plus 2-PMPA is completely blocked by the Group II metabotropic glutamate receptor antagonist (S)-alpha-ethylglutamate but is not altered by antagonists of Group I or III metabotropic glutamate receptors. The N-methyl-D-aspartate receptor antagonist MK801 reduces but does not eliminate the hyperpolarization generated by glutamate, NAAG or stimulation. These results, in combination with those of the preceding paper, are consistent with the premise that NAAG could be the primary axon-to-glia signaling agent. When the unstimulated nerve fiber is treated with cysteate, a glutamate reuptake blocker, there is a small hyperpolarization of the glial cell that can be substantially reduced by pretreatment with 2-PMPA before addition of cysteate. A similar effect of cysteate is seen during a 50 Hz/5 s stimulation. From these results we suggest that glutamate derived from NAAG hydrolysis appears in the periaxonal space under the conditions of these experiments and may contribute to the glial hyperpolarization.


Subject(s)
Aspartic Acid/analogs & derivatives , Astacoidea/metabolism , Axons/metabolism , Cell Communication/physiology , Dipeptides/metabolism , Nervous System/metabolism , Neuroglia/metabolism , Signal Transduction/physiology , Animals , Aspartic Acid/metabolism , Aspartic Acid/pharmacology , Astacoidea/cytology , Astacoidea/drug effects , Axons/drug effects , Carboxypeptidases/antagonists & inhibitors , Carboxypeptidases/metabolism , Cell Communication/drug effects , Cysteic Acid/pharmacology , Dipeptides/pharmacology , Enzyme Inhibitors/pharmacology , Excitatory Amino Acid Antagonists/pharmacology , Glutamate Carboxypeptidase II , Glutamic Acid/metabolism , Glutamic Acid/pharmacology , Membrane Potentials/drug effects , Membrane Potentials/physiology , N-Methylaspartate/pharmacology , Nervous System/cytology , Nervous System/drug effects , Neuroglia/drug effects , Organophosphorus Compounds/pharmacology , Receptors, Glutamate/drug effects , Receptors, Glutamate/metabolism , Signal Transduction/drug effects , Time Factors
15.
Neuroscience ; 106(1): 237-47, 2001.
Article in English | MEDLINE | ID: mdl-11564433

ABSTRACT

Early physiological and pharmacological studies of crayfish and squid giant nerve fibers suggested that glutamate released from the axon during action potential generation initiates metabolic and electrical responses of periaxonal glia. However, more recent investigations in our laboratories suggest that N-acetylaspartylglutamate (NAAG) may be the released agent active at the glial cell membrane. The investigation described in this paper focused on NAAG metabolism and release, and its contribution to the appearance of glutamate extracellularly. Axoplasm and periaxonal glial cell cytoplasm collected from medial giant nerve fibers (MGNFs) incubated with radiolabeled L-glutamate contained radiolabeled glutamate, glutamine, NAAG, aspartate, and GABA. Total radiolabel release was not altered by electrical stimulation of nerve cord loaded with [(14)C]glutamate by bath application or loaded with [(14)C]glutamate, [(3)H]-D-aspartate or [(3)H]NAAG by axonal injection. However, when radiolabeled glutamate was used for bath loading, radiolabel distribution among glutamate and its metabolic products in the superfusate was changed by stimulation. NAAG was the largest fraction, accounting for approximately 50% of the total recovered radiolabel in control conditions. The stimulated increase in radioactive NAAG in the superfusate coincided with its virtual clearance from the medial giant axon (MGA). A small, stimulation-induced increase in radiolabeled glutamate in the superfusate was detected only when a glutamate uptake inhibitor was present. The increase in [(3)H]glutamate in the superfusion solution of nerve incubated with [(3)H]NAAG was reduced when beta-NAAG, a competitive glutamate carboxypeptidase II (GCP II) inhibitor, was present.Overall, these results suggest that glutamate is metabolized to NAAG in the giant axon and its periaxonal glia and that, upon stimulation, NAAG is released from the axon and converted in part to glutamate by GCP II. A quisqualate- and beta-NAAG-sensitive GCP II activity was detected in nerve cord homogenates. These results, together with those in the accompanying paper demonstrating that NAAG can activate a glial electrophysiological response comparable to that initiated by glutamate, implicate NAAG as a probable mediator of interactions between the MGA and its periaxonal glia.


Subject(s)
Astacoidea/metabolism , Axons/metabolism , Cell Communication/physiology , Dipeptides/biosynthesis , Nervous System/metabolism , Neuroglia/metabolism , Signal Transduction/physiology , Animals , Aspartic Acid/metabolism , Astacoidea/cytology , Astacoidea/drug effects , Axons/drug effects , Carbon Radioisotopes/metabolism , Carboxypeptidases/drug effects , Carboxypeptidases/metabolism , Cell Communication/drug effects , Culture Media/chemistry , Culture Media/pharmacology , Cytoplasm/drug effects , Cytoplasm/metabolism , Dipeptides/metabolism , Dipeptides/pharmacokinetics , Electric Stimulation , Glutamate Carboxypeptidase II , Glutamic Acid/metabolism , Glutamic Acid/pharmacokinetics , Glutamine/metabolism , Nervous System/cytology , Nervous System/drug effects , Neuroglia/drug effects , Organ Culture Techniques , Quisqualic Acid/pharmacology , Signal Transduction/drug effects , Tritium/metabolism , gamma-Aminobutyric Acid/metabolism
16.
Neurosci Behav Physiol ; 31(4): 451-5, 2001.
Article in English | MEDLINE | ID: mdl-11508498

ABSTRACT

Experiments on rat diaphragm muscle showed that the nitric oxide (NO) donors sodium nitroprusside SNP) and S-nitroso-N-acetylpenicillamine (SNAP). as well as L-arginine. a substrate for NO synthesis. decreased the level of muscle fiber hyperpolarization (the H effect) after blockade of cholinoceptors on the postsynaptic membrane by d-tubocurarine in conditions of irreversible inhibition of acetylcholinesterase with armine. Conversely, disruptions to NO synthesis in muscle fibers by the NO synthase blocker NG-nitro-L-arginine methyl ester (L-NAME) led to increases in the H effect both in vitro and in vivo. Inactivated solutions of sodium nitroprusside and inactive forms of arginine and NAME (D-arginine. D-NAME) had no effect on the magnitude of the H effect, while hemoglobin, which efficiently binds NO molecules, blocked the inhibitory effects of sodium nitroprusside. SNAP, and L-arginine on the magnitude of the H effect. All these points provide evidence that NO can function as a modulator of non-quantum mediator release in the neuromuscular junctions of warm-blooded animals.


Subject(s)
Neuromuscular Junction/physiology , Neurotransmitter Agents/physiology , Nitric Oxide/physiology , Acetylcholine/metabolism , Animals , Enzyme Inhibitors/pharmacology , Excitatory Postsynaptic Potentials/drug effects , Extracellular Space/physiology , In Vitro Techniques , Membrane Potentials/drug effects , Motor Neurons/physiology , Muscle, Skeletal/innervation , Muscle, Skeletal/physiology , NG-Nitroarginine Methyl Ester/pharmacology , Nerve Endings/physiology , Neuromuscular Junction/metabolism , Neurotransmitter Agents/metabolism , Nitric Oxide/biosynthesis , Nitric Oxide Donors/pharmacology , Nitric Oxide Synthase/antagonists & inhibitors , Nitric Oxide Synthase Type III , Rats , Synapses/drug effects , Synapses/physiology
17.
Ross Fiziol Zh Im I M Sechenova ; 87(4): 492-8, 2001 Apr.
Article in Russian | MEDLINE | ID: mdl-11449976

ABSTRACT

In rats, glutamate was shown to exert no effect on the mean frequency, character of interstimuli distribution, amplitude and temporal parameters of the miniature EPPs. Glutamate suppressed nonquantal release. The glutamate effect depended on its concentration and was abolished by blockade of NMDA receptors, NO-synthase inhibitoin, and NO molecules binding by haemoglobin in extracellular medium. Glutamate seems to modulate the nonquantal acetylcholine secretion by initiation of the NO synthesis in muscle fibres via activation of the NMDA receptors.


Subject(s)
Acetylcholine/metabolism , Glutamic Acid/physiology , Neuromuscular Junction/metabolism , Animals , Diaphragm/physiology , Diaphragm/ultrastructure , Enzyme Inhibitors/pharmacology , Glutamic Acid/pharmacology , Heme/metabolism , In Vitro Techniques , Motor Endplate/physiology , Neuromuscular Junction/drug effects , Nitric Oxide/metabolism , Nitric Oxide Synthase/antagonists & inhibitors , Rats
18.
Ross Fiziol Zh Im I M Sechenova ; 87(4): 476-91, 2001 Apr.
Article in Russian | MEDLINE | ID: mdl-11449975

ABSTRACT

Studies of crayfish Medial Giant nerve Fiber suggested that glutamate (GLU) released from the axon during action potential generation initiates metabolic and electrical responses of periaxonal glia. This investigation sought to elucidate the mechanism of GLU appearance extracellularly following axon stimulation. Axoplasm and periaxonal glial sheath from nerve fibers incubated with radiolabelled L-GLU contained radiolabeled GLU, glutamine (GLN), GABA, aspartate (ASP), and NAAG. Total radiolabel release was not altered by electrical stimulation of nerve cord loaded with [14C]-GLU by bath application or loaded with [14C]-GLU, [3H]-D-ASP, or [3H]-NAAG by axonal injection. However, radioactivity distribution among GLU and its metabolic products in the superfusate was changed, with NAAG accounting for the largest fraction. In axons incubated with radiolabeled GLU, the stimulated increase in radioactive NAAG in the superfusate coincided with the virtual clearance of radioactive NAAG from the axon. The increase in [3H]-GLU in the superfusion solution that was seen upon stimulation of nerve bathloaded with [3H]-NAAG was reduced when beta-NAAG, a competitive NAALADase inhibitor, was present. Together, these results suggest that some GLU is metabolized to NAAG in the giant axon and its periaxonal glia and that, upon stimulation, NAAG is released and converted to GLU by NAALADase. A quisqualate-, beta-NAAG-sensitive NAALADase activity was detected in nerve cord homogenates. Stimulation or NAAG administration in the presence of NAALADase inhibitor caused a transient hyperpolarization of the periaxonal glia comparable to that produced by L-GLU. The results implicate N-acetylaspartylglutamate (NAAG) and GLU as potential mediators. of the axon-glia interactions.


Subject(s)
Axons/metabolism , Dipeptides/metabolism , Action Potentials , Axons/drug effects , Carboxypeptidases/antagonists & inhibitors , Carboxypeptidases/metabolism , Cell Membrane/physiology , Chromatography, High Pressure Liquid , Dipeptides/pharmacology , Electric Stimulation , Enzyme Inhibitors/pharmacology , Extracellular Space/metabolism , Ganglia, Invertebrate/drug effects , Ganglia, Invertebrate/metabolism , Ganglia, Invertebrate/ultrastructure , Glutamate Carboxypeptidase II , Glutamic Acid/metabolism , Hydrolysis , In Vitro Techniques , Neuroglia/metabolism , Neuroglia/ultrastructure
19.
Neurosci Res ; 37(4): 255-63, 2000 Aug.
Article in English | MEDLINE | ID: mdl-10958974

ABSTRACT

The resting membrane potential (RMP) of denervated muscle fibres of rat diaphragm muscle is depolarized by approximately 8-10 mV during the first 3 h after nerve section and this early postdenervation depolarization is reduced substantially by the presence of 5x10(-8) M acetylcholine (ACh) or carbachol (CB). The muscarinic antagonist atropine (Atr; 5x10(-9) to 5x10(-6) M) reduced the effect of CB in a dose-dependent manner (K(i)=7x10(-8) M) and increased the rate of the early postdenervation depolarization. In lower doses (5x10(-7) M), Atr acted only in the presence of an allosteric stabilizator hexamethylene-bis-[dimethyl-(3-phtalimidopropyl)ammonium] (W-84). Also pirenzepine, a specific inhibitor of the M1 subtype of muscarinic receptor, blocked the action of CB in a dose-dependent manner with an apparent inhibition constant K(i)=1x10(-7) microM. DAMP, a specific M3 antagonist, was without effect on the muscle hyperpolarization induced by CB. CB also hyperpolarized the membrane potentials of muscles which were denervated for 1-3 days. It is concluded that ACh and CB protect the muscle fibres from early depolarization through M1-cholinergic receptors on the muscle membrane. These particular receptors can apparently mediate the 'trophic', non-impulse regulation of RMP in skeletal muscles when they are activated by acetylcholine released non-quantally.


Subject(s)
Acetylcholine/pharmacology , Arecoline/analogs & derivatives , Carbachol/pharmacology , Muscle Fibers, Skeletal/physiology , Receptors, Muscarinic/physiology , Animals , Arecoline/pharmacology , Atropine/pharmacology , Culture Techniques , Diaphragm/innervation , Diaphragm/ultrastructure , Male , Membrane Potentials , Muscarinic Agonists/pharmacology , Muscarinic Antagonists/pharmacology , Muscle Denervation , Oxotremorine/pharmacology , Piperidines/pharmacology , Pirenzepine/pharmacology , Rats , Rats, Wistar , Receptor, Muscarinic M1
20.
Neuroscience ; 97(3): 601-9, 2000.
Article in English | MEDLINE | ID: mdl-10828542

ABSTRACT

In crayfish and squid giant nerve fibers, glutamate appears to be an axon-glia signaling agent. We have investigated glutamate transport and metabolism by crayfish central nerve fibers in order to identify possible mechanisms by which glutamate could subserve this non-synaptic signaling function. Accumulation of radiolabeled L-glutamate by desheathed cephalothoracic nerve bundles was temperature and Na(+) dependent, linear with time for at least 8h and saturable at about 0.5-1mM L-glutamate. Most accumulated radiotracer was associated with the periaxonal glial sheath and remained as glutamate. Compounds known to block glutamate transport in invertebrate peripheral nerves or mammalian brain slices or cell cultures were also effective on crayfish central nerve fibers. Tissue radiotracer levels were only 3% of control levels when 1mM p-chloromercuriphenylsulfonate was present, and 13%, 20%, 26%, 38% and 42% of control levels, respectively, when L-cysteate, L-cysteine sulfinate, L-aspartate, D-aspartate or DL-threo-beta-hydroxyaspartate was present. L-Glutamine, GABA, N-methyl-DL-aspartate, alpha-aminoadipate and D-glutamate were without inhibitory effect on tissue tracer accumulation. Radiolabeled D-aspartate was an equivalent non-metabolized substitute for radiolabeled L-glutamate. D-Aspartate, p-chloromercuriphenylsulfonate and GABA had comparable effects on isolated medial giant nerve fibers.These studies indicate that L-glutamate is taken up primarily by the periaxonal glia of crayfish central nerve fibers by a low-affinity, saturable, Na(+)-dependent transport system and is retained by the fibers primarily in that form. Our results suggest that the glia are not only the target of the glutamate signal released from non-synaptic regions of the crayfish medial giant axon during high-frequency stimulation, but that they are also the primary site of its inactivation.


Subject(s)
Astacoidea/metabolism , Axons/metabolism , Glutamic Acid/metabolism , Neuroglia/metabolism , Signal Transduction/physiology , Animals , Aspartic Acid/metabolism , Astacoidea/cytology , Attention/physiology , Axons/drug effects , Axons/ultrastructure , Body Temperature/physiology , Excitatory Amino Acid Agonists/pharmacology , Ganglia, Invertebrate/cytology , Ganglia, Invertebrate/drug effects , Ganglia, Invertebrate/metabolism , Inulin/pharmacology , Neuroglia/cytology , Neuroglia/drug effects , Sodium/metabolism , Time Factors , Tritium
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