ABSTRACT
The technique of immune blotting was used to analyze surface proteins obtained from cells M Tuberculosis exposed to partialmode of delipidization. At that, there were applied serums of patients with tuberculosis of lungs; HIV agents and patients with concomitant infection tuberculosis-AIDS and also HIV-negative patients without clinical signs of disease of lungs and with chronic diseases of lungs of other etiology The fractions oflow-molecular antigens with molecular mass 6.5-30 kilodaltons became diagnostically significant. To this fraction of antigens reacted serums of all patients with tuberculosis of lungs and serums of 91% of patients with concomitant tuberculosis-AIDS infection. The antigens of protein fractions with high (70-100 kilodaltons) and interim (30-69 kilodaltons) molecular mass became diagnostically insignificant.
Subject(s)
Antigen-Antibody Complex/blood , Antigens, Bacterial/isolation & purification , Immune Sera/chemistry , Mycobacterium tuberculosis/chemistry , Tuberculosis, Pulmonary/diagnosis , Adult , Antigens, Bacterial/immunology , Asthma/blood , Asthma/diagnosis , Asthma/immunology , Case-Control Studies , Coinfection , Diagnosis, Differential , Electrophoresis, Polyacrylamide Gel , Female , HIV Infections/blood , HIV Infections/diagnosis , HIV Infections/immunology , HIV Infections/virology , HIV-1/physiology , Humans , Male , Mycobacterium tuberculosis/immunology , Pneumonia, Bacterial/blood , Pneumonia, Bacterial/diagnosis , Pneumonia, Bacterial/immunology , Pulmonary Disease, Chronic Obstructive/blood , Pulmonary Disease, Chronic Obstructive/diagnosis , Pulmonary Disease, Chronic Obstructive/immunology , Tuberculosis, Pulmonary/blood , Tuberculosis, Pulmonary/immunology , Tuberculosis, Pulmonary/microbiologyABSTRACT
Treatment with dimethyl suberimidate, a cross-linking bifunctional agent, showed that Sm1 and Sm2 nucleases of Serratia marcescens B10M1 are polydisperse in solution and consist of monomers and dimers at the level of pH optimal for the enzyme activity. The data suggest that nucleases from the strain B10M1 and any other strain are polydisperse at pH optimum if their amino acid sequences are identical.
Subject(s)
Endodeoxyribonucleases/metabolism , Endoribonucleases/metabolism , Amino Acid Sequence , Cross-Linking Reagents/chemistry , Dimerization , Dimethyl Suberimidate/chemistry , Electrophoresis, Polyacrylamide Gel , Endodeoxyribonucleases/chemistry , Endoribonucleases/chemistry , Hydrogen-Ion Concentration , Molecular Sequence DataABSTRACT
Two enzyme forms of endonuclease (Sm 1 and Sm 2) strain B10M1 in 60 and 100 mg respectively have been isolated from the culture fluid Serratia marcescens. The chromatographic and electrophoretic properties and N-terminal amino acid residues are different for both enzymes. The purification procedure consists of dialysis and ion-exchange chromatography on DEAE- and phosphocellulose. The yield of nucleases Sm1 and Sm2 are 14% and 28% respectively. The antigenic differences of nucleases Sm1 and Sm2 have been found by cross immunoenzyme analysis.
