Calidad de vida en posmenopáusicas tratadas con tibolona / Quality of life in postmenopausal women treated with tibolone

Comparar la calidad de vida (CV) en mujeres posmenopáusicas antes y después del tratamiento con Tibolona atendidas en la consulta ginecológica de la Maternidad "Dr. Armando Castillo Plaza", Maracaibo, Venezuela. Métodos: Investigación comparativa y aplicada, con diseño no experimental, y prospectivo, que incluyo una muestra de 100 pacientes posmenopáusicas, evaluadas antes y después de 6 y 12 meses de recibir la terapia de reemplazo hormonal con Tibolona oral (2,5mg/día) por medio del cuestionario Menopause Rating Scale (MRS).Resultados: 94 mujeres completaron el protocolo de estudio, determinándose que antes de comenzar el tratamiento la mayoría presentaba síntomas somáticos, psicológicos y genitourinarios severos, mientras que al cabo de 12 meses de tratamiento hubo una mejoría significativa en todos los síntomas estudiados (P= 0.000). Asimismo, se evidenció una mejora significativa en la CV luego del tratamiento con Tibolona, expresada por una regresión de las mediasen las puntuaciones alcanzadas con el MRS entre la medición inicial, a los 6 y a los 12 meses; con un porcentaje de cambio en la CV de las pacientes participantes de -38,33% y -82.20% después de 6 y 12 meses de la intervención farmacológica, los cuales resultaron de relevancia clínica y significancia estadística (P= 0.000). Conclusión: La terapia hormonal sustitutiva con Tibolona mejora la CV de las pacientes posmenopáusicas en todas sus dimensiones, sobre todo en cuanto a la disminución de los síntomas somáticos.Palabras clave: calidad de vida, menopausia, terapia de reemplazo hormonal, tibolona(AU)

To compare the quality of life (QOL) in postmenopausal women before and after treatment with Tibolone attending at the gynaecological consultation in the "Maternidad Dr. Armando Castillo Plaza", Maracaibo, Venezuela. Methods: Comparative and applied research with quasi experimental and prospective design, which included a sample of 100 postmenopausal women, evaluated before and after 6 and 12 months receiving hormone replacement therapy with oral Tibolone (2,5mg/day) through the questionnaire Menopause Rating Scale (MRS). Results: 94 women completed the study protocol, it was found that before treatment the majority had most somatic, psychological and severe genitourinary, while after 12 months of treatment there was a significant improvement in all symptoms studied (P = 0.000 .) We also demonstrated a significant improvement in QOL after treatment with Tibolone, expressed by a regression of the means of the scores obtained with the MRS between the initial measurement, at 6 and 12 months, with a percentage change in the CV of the patients participating -38.33% -82.20% and after 6 and 12 months of pharmacological intervention, which were of clinical relevance and statistical significance (P = 0.000). Conclusion: Hormone replacement therapy with Tibolone improves QOL of the postmenopausal patients in all its dimensions, especially in terms of reduction in somatic symptoms(AU)

The effect of tunicamycin on Leishmania brasiliensis. Glycosylation and the cell surface components.

Culture conditions of Leishmania cells were developed to allow the study of the effect of tunicamycin (TM) on glycosylation and on the cell surface components. Leishmania incorporate [14C]-mannose and [35S]-methionine in vitro. The incorporation of [14C]-mannose is linear for 150 min and is inhibited by TM (2 micrograms/ml) in a time dependent effect which reaches a plateau of 45% inhibition at 36 h. Under the same experimental conditions [35S]-methionine incorporation into protein is slightly affected. This is reflected by an almost identical polypeptide pattern for TM treated and non-treated cells when analyzed on SDS-PAGE. On the contrary, strong differences were detected on the labeled compounds analyzed on SDS-PAGE followed by autoradiography when the precursor used was [14C]-mannose. A shift in the electrophoretic mobility of most of the glycopeptides synthesized in the presence of TM was observed, which is also reflected in the structure of the main Leishmania cell surface components. The findings are discussed in the light of biological implications.